##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbeI
BbrPI BcgI BciVI BsaAI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRV FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SfoI
SfuI SgrAI SnaBI SpeI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 373 CTTAAG C^TTAAG
AspHI 5472 GAGCTC G[AT]GC[AT]^C
AvrII 4800 CCTAGG C^CTAGG
BanI 5264 GGTGCC G^G[CT][GA]CC
BfrI 373 CTTAAG C^TTAAG
BglI 3928 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4800 CCTAGG C^CTAGG
BsaBI 1602 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5199 CGGCCG CG[GA][CT]^CG
BsiHKAI 5472 GAGCTC G[AT]GC[AT]^C
BspHI 4253 TCATGA T^CATGA
BsrBI 1493 CCGCTC CCGCTC(-3/-3)
BsrFI 4901 ACCGGC [GA]^CCGG[CT]
BstAPI 190 GCAATTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2315 CCAAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3357 GCGC GCG^C
Cfr10I 4901 ACCGGC [GA]^CCGG[CT]
DraIII 725 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5199 CGGCCG C^GGCCG
EclXI 5199 CGGCCG C^GGCCG
Eco52I 5199 CGGCCG C^GGCCG
FauI 1492 CCCGC CCCGC(4/6)
HhaI 3357 GCGC GCG^C
HinP1I 3357 GCGC G^CGC
MamI 1602 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1829 CCATGG C^CATGG
NotI 5198 GCGGCCGC GC^GGCCGC
PflMI 951 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 4253 TCATGA T^CATGA
SacI 5472 GAGCTC GAGCT^C
SalI 1760 GTCGAC G^TCGAC
SbfI 180 CCTGCAGG CCTGCA^GG
SfiI 3927 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 180 CCTGCAGG CCTGCA^GG
Van91I 951 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 120 ACATGT A^C[GA][CT]GT
4765 ACATGT
AlwI 3606 GATCC GGATC(4/5)
506 GGATC
AocI 2019 CCTGAGG CC^T[ACGT]AGG
3738 CCTGAGG
AspI 1481 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACGGTGTC
AvaI 3929 CCCGGG C^[CT]CG[GA]G
5000 CCCGGG
BalI 2249 TGGCCA TGG^CCA
3771 TGGCCA
BanII 88 GGGCTC G[GA]GC[CT]^C
5472 GAGCTC
BsaWI 2792 TCCGGT [AT]^CCGG[AT]
2981 ACCGGA
BsoBI 3929 CCCGGG C^[CT]CG[GA]G
5000 CCCGGG
BspLU11I 120 ACATGT A^CATGT
4765 ACATGT
Bsu36I 2019 CCTGAGG CC^T[ACGT]AGG
3738 CCTGAGG
DraI 2841 TTTAAA TTT^AAA
5330 TTTAAA
DrdI 31 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2631 GACTCAGATGTC
DsaI 1829 CCATGG C^C[GA][CT]GG
2602 CCACGG
Eco81I 2019 CCTGAGG CC^T[ACGT]AGG
3738 CCTGAGG
EcoT14I 1829 CCATGG C^C[AT][AT]GG
4800 CCTAGG
HincII 484 GTTGAC GT[CT]^[GA]AC
1760 GTCGAC
HindII 484 GTTGAC GT[CT]^[GA]AC
1760 GTCGAC
MfeI 191 CAATTG C^AATTG
803 CAATTG
MluNI 2249 TGGCCA TGG^CCA
3771 TGGCCA
MscI 2249 TGGCCA TGG^CCA
3771 TGGCCA
MunI 191 CAATTG C^AATTG
803 CAATTG
NheI 969 GCTAGC G^CTAGC
4125 GCTAGC
PflFI 1481 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACGGTGTC
SapI 3076 GAAGAGC GCTCTTC(1/4)
5474 GCTCTTC
ScaI 2244 AGTACT AGT^ACT
3948 AGTACT
SexAI 726 ACCAGGT A^CC[AT]GGT
1560 ACCAGGT
SmaI 3929 CCCGGG CCC^GGG
5000 CCCGGG
SphI 1169 GCATGC GCATG^C
3278 GCATGC
SspI 2837 AATATT AAT^ATT
4613 AATATT
StyI 1829 CCATGG C^C[AT][AT]GG
4800 CCTAGG
Tth111I 1481 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACGGTGTC
XmaCI 3929 CCCGGG C^CCGGG
5000 CCCGGG
XmaI 3929 CCCGGG C^CCGGG
5000 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI Asp700I AvaII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI