################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII Acc65I AgeI AscI Asp718I AspI AviII AvrII BbeI BcgI BciVI BclI BlnI BsiWI BsmI Bsp1407I BspDI BsrGI BssHII BstBI ClaI CpoI EcoRV FbaI FseI FspI HpaI KasI KpnI MfeI MunI NarI NdeI NruI NspV PacI PaeR7I PflFI PinAI PmeI RsrII SacI SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 950 TCCGGA T^CCGGA AclI 5168 AACGTT AA^CGTT AflII 5320 CTTAAG C^TTAAG AhdI 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 815 GTGCAC G^TGCAC ApaLI 815 GTGCAC G^TGCAC AseI 506 ATTAAT AT^TAAT AsnI 506 ATTAAT AT^TAAT Asp700I 2235 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BfrI 5320 CTTAAG C^TTAAG BglII 2560 AGATCT A^GATCT BlpI 1206 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1206 GCTCAGC GC^T[ACGT]AGC BseAI 950 TCCGGA T^CCGGA BsmBI 4092 CGTCTC CGTCTC(1/5) BspEI 950 TCCGGA T^CCGGA BsrDI 4268 GCAATG GCAATG(2/0) Bst1107I 5530 GTATAC GTA^TAC BstEII 4752 GGTTACC G^GT[ACGT]ACC BstPI 4752 GGTTACC G^GT[ACGT]ACC BstZ17I 5530 GTATAC GTA^TAC CelII 1206 GCTCAGC GC^T[ACGT]AGC DraI 3202 TTTAAA TTT^AAA DraIII 2213 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4008 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4752 GGTTACC G^GT[ACGT]ACC EcoT22I 4175 ATGCAT ATGCA^T HindIII 2389 AAGCTT A^AGCTT KspI 1161 CCGCGG CCGC^GG MluI 5209 ACGCGT A^CGCGT MroI 950 TCCGGA T^CCGGA NaeI 5434 GCCGGC GCC^GGC NcoI 744 CCATGG C^CATGG NgoMIV 5434 GCCGGC G^CCGGC NheI 1211 GCTAGC G^CTAGC NotI 2626 GCGGCCGC GC^GGCCGC NsiI 4175 ATGCAT ATGCA^T PshBI 506 ATTAAT AT^TAAT Psp1406I 5168 AACGTT AA^CGTT PvuI 3938 CGATCG CGAT^CG SacII 1161 CCGCGG CCGC^GG ScaI 5324 AGTACT AGT^ACT SfiI 2369 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1378 CCCGGG CCC^GGG SspI 5546 AATATT AAT^ATT XmaCI 1378 CCCGGG C^CCGGG XmaI 1378 CCCGGG C^CCGGG XmnI 2235 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 5398 GTAGAC GT^[AC][GT]AC 5530 GTATAC Aor51HI 2488 AGCGCT AGC^GCT 5148 AGCGCT ApaI 2136 GGGCCC GGGCC^C 2368 GGGCCC BamHI 570 GGATCC G^GATCC 4110 GGATCC BbvCI 3690 CCTCAGC CCTCAGC(-5/-2) 2491 GCTGAGG BsaBI 1619 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4295 GATTTTTATC BspHI 2185 TCATGA T^CATGA 2532 TCATGA BspLU11I 648 ACATGT A^CATGT 2952 ACATGT BspMI 1612 GCAGGT ACCTGC(4/8) 2355 GCAGGT BssSI 679 CACGAG CACGAG(-5/-1) 4853 CTCGTG DrdI 2282 GACAGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4842 GACAAGAAAGTC EagI 2329 CGGCCG C^GGCCG 2627 CGGCCG EclXI 2329 CGGCCG C^GGCCG 2627 CGGCCG Eco47III 2488 AGCGCT AGC^GCT 5148 AGCGCT Eco52I 2329 CGGCCG C^GGCCG 2627 CGGCCG MamI 1619 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4295 GATTTTTATC PflMI 2212 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3891 CCATGTCCTGG RcaI 2185 TCATGA T^CATGA 2532 TCATGA SapI 2942 GAAGAGC GCTCTTC(1/4) 4733 GCTCTTC Van91I 2212 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3891 CCATGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII