##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AgeI AscI Asp718I
AspI AviII AvrII BbeI BcgI
BciVI BclI BlnI BsiWI BsmI
Bsp1407I BspDI BsrGI BssHII BstBI
ClaI CpoI EcoRV FbaI FseI
FspI HpaI KasI KpnI MfeI
MunI NarI NdeI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
RsrII SacI SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 950 TCCGGA T^CCGGA
AclI 5168 AACGTT AA^CGTT
AflII 5320 CTTAAG C^TTAAG
AhdI 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 815 GTGCAC G^TGCAC
ApaLI 815 GTGCAC G^TGCAC
AseI 506 ATTAAT AT^TAAT
AsnI 506 ATTAAT AT^TAAT
Asp700I 2235 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 5320 CTTAAG C^TTAAG
BglII 2560 AGATCT A^GATCT
BlpI 1206 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1206 GCTCAGC GC^T[ACGT]AGC
BseAI 950 TCCGGA T^CCGGA
BsmBI 4092 CGTCTC CGTCTC(1/5)
BspEI 950 TCCGGA T^CCGGA
BsrDI 4268 GCAATG GCAATG(2/0)
Bst1107I 5530 GTATAC GTA^TAC
BstEII 4752 GGTTACC G^GT[ACGT]ACC
BstPI 4752 GGTTACC G^GT[ACGT]ACC
BstZ17I 5530 GTATAC GTA^TAC
CelII 1206 GCTCAGC GC^T[ACGT]AGC
DraI 3202 TTTAAA TTT^AAA
DraIII 2213 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4520 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4008 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4752 GGTTACC G^GT[ACGT]ACC
EcoT22I 4175 ATGCAT ATGCA^T
HindIII 2389 AAGCTT A^AGCTT
KspI 1161 CCGCGG CCGC^GG
MluI 5209 ACGCGT A^CGCGT
MroI 950 TCCGGA T^CCGGA
NaeI 5434 GCCGGC GCC^GGC
NcoI 744 CCATGG C^CATGG
NgoMIV 5434 GCCGGC G^CCGGC
NheI 1211 GCTAGC G^CTAGC
NotI 2626 GCGGCCGC GC^GGCCGC
NsiI 4175 ATGCAT ATGCA^T
PshBI 506 ATTAAT AT^TAAT
Psp1406I 5168 AACGTT AA^CGTT
PvuI 3938 CGATCG CGAT^CG
SacII 1161 CCGCGG CCGC^GG
ScaI 5324 AGTACT AGT^ACT
SfiI 2369 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1378 CCCGGG CCC^GGG
SspI 5546 AATATT AAT^ATT
XmaCI 1378 CCCGGG C^CCGGG
XmaI 1378 CCCGGG C^CCGGG
XmnI 2235 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 5398 GTAGAC GT^[AC][GT]AC
5530 GTATAC
Aor51HI 2488 AGCGCT AGC^GCT
5148 AGCGCT
ApaI 2136 GGGCCC GGGCC^C
2368 GGGCCC
BamHI 570 GGATCC G^GATCC
4110 GGATCC
BbvCI 3690 CCTCAGC CCTCAGC(-5/-2)
2491 GCTGAGG
BsaBI 1619 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4295 GATTTTTATC
BspHI 2185 TCATGA T^CATGA
2532 TCATGA
BspLU11I 648 ACATGT A^CATGT
2952 ACATGT
BspMI 1612 GCAGGT ACCTGC(4/8)
2355 GCAGGT
BssSI 679 CACGAG CACGAG(-5/-1)
4853 CTCGTG
DrdI 2282 GACAGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4842 GACAAGAAAGTC
EagI 2329 CGGCCG C^GGCCG
2627 CGGCCG
EclXI 2329 CGGCCG C^GGCCG
2627 CGGCCG
Eco47III 2488 AGCGCT AGC^GCT
5148 AGCGCT
Eco52I 2329 CGGCCG C^GGCCG
2627 CGGCCG
MamI 1619 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4295 GATTTTTATC
PflMI 2212 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3891 CCATGTCCTGG
RcaI 2185 TCATGA T^CATGA
2532 TCATGA
SapI 2942 GAAGAGC GCTCTTC(1/4)
4733 GCTCTTC
Van91I 2212 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3891 CCATGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII