##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccIII AclI AhdI ApaI AscI
AseI AsnI AspEI BbsI BciVI
BclI BpuAI BsaBI BseAI BsiWI
BspEI BspLU11I Bst1107I BstBI BstZ17I
DraI Eam1105I EcoRI FbaI FseI
HindIII HpaI MamI MluI MroI
NruI NspI NspV PacI PflMI
PmeI PshAI PshBI Psp1406I PvuI
SalI SfuI SnaBI SpeI SphI
SspI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1575 GACGTC GACGT^C
Acc65I 4177 GGTACC G^GTACC
AcsI 1311 GAATTT [GA]^AATT[CT]
AflII 2627 CTTAAG C^TTAAG
AgeI 2560 ACCGGT A^CCGGT
ApoI 1311 GAATTT [GA]^AATT[CT]
Asp718I 4177 GGTACC G^GTACC
AvrII 1505 CCTAGG C^CTAGG
BbvCI 2451 GCTGAGG CCTCAGC(-5/-2)
BfrI 2627 CTTAAG C^TTAAG
BglII 2435 AGATCT A^GATCT
BlnI 1505 CCTAGG C^CTAGG
BsaWI 2560 ACCGGT [AT]^CCGG[AT]
BsmI 3506 GCATTC GAATGC(1/-1)
BspDI 2418 ATCGAT AT^CGAT
BspHI 2734 TCATGA T^CATGA
BssSI 1530 CTCGTG CACGAG(-5/-1)
ClaI 2418 ATCGAT AT^CGAT
CpoI 4425 CGGACCG CG^G[AT]CCG
EcoNI 2447 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 115 GATATC GAT^ATC
EcoT22I 3249 ATGCAT ATGCA^T
KpnI 4177 GGTACC GGTAC^C
MfeI 4066 CAATTG C^AATTG
MunI 4066 CAATTG C^AATTG
NdeI 2725 CATATG CA^TATG
NheI 1115 GCTAGC G^CTAGC
NsiI 3249 ATGCAT ATGCA^T
PaeR7I 2778 CTCGAG C^TCGAG
PinAI 2560 ACCGGT A^CCGGT
RcaI 2734 TCATGA T^CATGA
RsrII 4425 CGGACCG CG^G[AT]CCG
SacI 606 GAGCTC GAGCT^C
SapI 2021 GCTCTTC GCTCTTC(1/4)
SbfI 5054 CCTGCAGG CCTGCA^GG
ScaI 4677 AGTACT AGT^ACT
SexAI 4852 ACCAGGT A^CC[AT]GGT
SfiI 4505 GGCCGACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 5054 CCTGCAGG CCTGCA^GG
StuI 1933 AGGCCT AGG^CCT
XhoI 2778 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 146 GTAGAC GT^[AC][GT]AC
1794 GTCTAC
AflIII 296 ACACGT A^C[GA][CT]GT
3361 ACGTGT
Alw44I 1284 GTGCAC G^TGCAC
3468 GTGCAC
Aor51HI 3866 AGCGCT AGC^GCT
3954 AGCGCT
ApaLI 1284 GTGCAC G^TGCAC
3468 GTGCAC
Asp700I 2558 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3569 GAATATGTTC
AviII 622 TGCGCA TGC^GCA
2838 TGCGCA
BamHI 170 GGATCC G^GATCC
1648 GGATCC
BlpI 608 GCTCAGC GC^T[ACGT]AGC
947 GCTCAGC
Bpu1102I 608 GCTCAGC GC^T[ACGT]AGC
947 GCTCAGC
BsmBI 2775 CGTCTC CGTCTC(1/5)
3012 GAGACG
Bsp1407I 2647 TGTACA T^GTACA
4819 TGTACA
BsrGI 2647 TGTACA T^GTACA
4819 TGTACA
BstEII 1583 GGTTACC G^GT[ACGT]ACC
1628 GGTGACC
BstPI 1583 GGTTACC G^GT[ACGT]ACC
1628 GGTGACC
CelII 608 GCTCAGC GC^T[ACGT]AGC
947 GCTCAGC
EagI 1011 CGGCCG C^GGCCG
1051 CGGCCG
EclXI 1011 CGGCCG C^GGCCG
1051 CGGCCG
Eco47III 3866 AGCGCT AGC^GCT
3954 AGCGCT
Eco52I 1011 CGGCCG C^GGCCG
1051 CGGCCG
EcoO65I 1583 GGTTACC G^GT[ACGT]ACC
1628 GGTGACC
FspI 622 TGCGCA TGC^GCA
2838 TGCGCA
NotI 1010 GCGGCCGC GC^GGCCGC
1050 GCGGCCGC
SmaI 555 CCCGGG CCC^GGG
2491 CCCGGG
SspBI 2647 TGTACA T^GTACA
4819 TGTACA
XmaCI 555 CCCGGG C^CCGGG
2491 CCCGGG
XmaI 555 CCCGGG C^CCGGG
2491 CCCGGG
XmnI 2558 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3569 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SgrAI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII