################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AccIII AclI AhdI ApaI AscI AseI AsnI AspEI BbsI BciVI BclI BpuAI BsaBI BseAI BsiWI BspEI BspLU11I Bst1107I BstBI BstZ17I DraI Eam1105I EcoRI FbaI FseI HindIII HpaI MamI MluI MroI NruI NspI NspV PacI PflMI PmeI PshAI PshBI Psp1406I PvuI SalI SfuI SnaBI SpeI SphI SspI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1575 GACGTC GACGT^C Acc65I 4177 GGTACC G^GTACC AcsI 1311 GAATTT [GA]^AATT[CT] AflII 2627 CTTAAG C^TTAAG AgeI 2560 ACCGGT A^CCGGT ApoI 1311 GAATTT [GA]^AATT[CT] Asp718I 4177 GGTACC G^GTACC AvrII 1505 CCTAGG C^CTAGG BbvCI 2451 GCTGAGG CCTCAGC(-5/-2) BfrI 2627 CTTAAG C^TTAAG BglII 2435 AGATCT A^GATCT BlnI 1505 CCTAGG C^CTAGG BsaWI 2560 ACCGGT [AT]^CCGG[AT] BsmI 3506 GCATTC GAATGC(1/-1) BspDI 2418 ATCGAT AT^CGAT BspHI 2734 TCATGA T^CATGA BssSI 1530 CTCGTG CACGAG(-5/-1) ClaI 2418 ATCGAT AT^CGAT CpoI 4425 CGGACCG CG^G[AT]CCG EcoNI 2447 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 115 GATATC GAT^ATC EcoT22I 3249 ATGCAT ATGCA^T KpnI 4177 GGTACC GGTAC^C MfeI 4066 CAATTG C^AATTG MunI 4066 CAATTG C^AATTG NdeI 2725 CATATG CA^TATG NheI 1115 GCTAGC G^CTAGC NsiI 3249 ATGCAT ATGCA^T PaeR7I 2778 CTCGAG C^TCGAG PinAI 2560 ACCGGT A^CCGGT RcaI 2734 TCATGA T^CATGA RsrII 4425 CGGACCG CG^G[AT]CCG SacI 606 GAGCTC GAGCT^C SapI 2021 GCTCTTC GCTCTTC(1/4) SbfI 5054 CCTGCAGG CCTGCA^GG ScaI 4677 AGTACT AGT^ACT SexAI 4852 ACCAGGT A^CC[AT]GGT SfiI 4505 GGCCGACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 5054 CCTGCAGG CCTGCA^GG StuI 1933 AGGCCT AGG^CCT XhoI 2778 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 146 GTAGAC GT^[AC][GT]AC 1794 GTCTAC AflIII 296 ACACGT A^C[GA][CT]GT 3361 ACGTGT Alw44I 1284 GTGCAC G^TGCAC 3468 GTGCAC Aor51HI 3866 AGCGCT AGC^GCT 3954 AGCGCT ApaLI 1284 GTGCAC G^TGCAC 3468 GTGCAC Asp700I 2558 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3569 GAATATGTTC AviII 622 TGCGCA TGC^GCA 2838 TGCGCA BamHI 170 GGATCC G^GATCC 1648 GGATCC BlpI 608 GCTCAGC GC^T[ACGT]AGC 947 GCTCAGC Bpu1102I 608 GCTCAGC GC^T[ACGT]AGC 947 GCTCAGC BsmBI 2775 CGTCTC CGTCTC(1/5) 3012 GAGACG Bsp1407I 2647 TGTACA T^GTACA 4819 TGTACA BsrGI 2647 TGTACA T^GTACA 4819 TGTACA BstEII 1583 GGTTACC G^GT[ACGT]ACC 1628 GGTGACC BstPI 1583 GGTTACC G^GT[ACGT]ACC 1628 GGTGACC CelII 608 GCTCAGC GC^T[ACGT]AGC 947 GCTCAGC EagI 1011 CGGCCG C^GGCCG 1051 CGGCCG EclXI 1011 CGGCCG C^GGCCG 1051 CGGCCG Eco47III 3866 AGCGCT AGC^GCT 3954 AGCGCT Eco52I 1011 CGGCCG C^GGCCG 1051 CGGCCG EcoO65I 1583 GGTTACC G^GT[ACGT]ACC 1628 GGTGACC FspI 622 TGCGCA TGC^GCA 2838 TGCGCA NotI 1010 GCGGCCGC GC^GGCCGC 1050 GCGGCCGC SmaI 555 CCCGGG CCC^GGG 2491 CCCGGG SspBI 2647 TGTACA T^GTACA 4819 TGTACA XmaCI 555 CCCGGG C^CCGGG 2491 CCCGGG XmaI 555 CCCGGG C^CCGGG 2491 CCCGGG XmnI 2558 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3569 GAATATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflFI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SgrAI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII