##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaI ApaLI Asp718I AviII
BbeI BbrPI BcgI BclI BglI
BlpI Bpu1102I BsaI BsiEI BsiWI
BspDI Bst1107I BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoNI FbaI FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SwaI XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 197 GACGCC G[GA]^CG[CT]C
AhdI 2933 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 382 GGCGCGCC GG^CGCGCC
AseI 2920 ATTAAT AT^TAAT
AsnI 2920 ATTAAT AT^TAAT
AspEI 2933 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 87 CCTAGG C^CTAGG
BalI 4511 TGGCCA TGG^CCA
BamHI 367 GGATCC G^GATCC
BbsI 2930 GAAGAC GAAGAC(2/6)
BbvCI 1141 CCTCAGC CCTCAGC(-5/-2)
BciVI 1494 GTATCC GTATCC(6/5)
BlnI 87 CCTAGG C^CTAGG
BpuAI 2930 GAAGAC GAAGAC(2/6)
BsaAI 3979 TACGTA [CT]AC^GT[GA]
BsaBI 1517 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 197 GACGCC G[GA]^CG[CT]C
BsrBI 766 CCGCTC CCGCTC(-3/-3)
BsrDI 2093 GCAATG GCAATG(2/0)
BsrFI 156 GCCGGC [GA]^CCGG[CT]
BssHII 383 GCGCGC G^CGCGC
Cfr10I 156 GCCGGC [GA]^CCGG[CT]
DraIII 2816 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 283 GACTTCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2933 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4078 GAATTC G^AATTC
HaeII 71 GGCGCT [GA]GCGC^[CT]
HgaI 197 GACGC GACGC(5/10)
Hin1I 197 GACGCC G[GA]^CG[CT]C
HincII 740 GTCAAC GT[CT]^[GA]AC
HindII 740 GTCAAC GT[CT]^[GA]AC
MamI 1517 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4511 TGGCCA TGG^CCA
MscI 4511 TGGCCA TGG^CCA
NaeI 156 GCCGGC GCC^GGC
NdeI 1770 CATATG CA^TATG
NgoMIV 156 GCCGGC G^CCGGC
PaeR7I 800 CTCGAG C^TCGAG
PflMI 1799 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1362 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2568 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2920 ATTAAT AT^TAAT
SacI 911 GAGCTC GAGCT^C
SnaBI 3979 TACGTA TAC^GTA
Van91I 1799 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 800 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1915 GTCTAC GT^[AC][GT]AC
4864 GTAGAC
AccIII 770 TCCGGA T^CCGGA
2194 TCCGGA
AlwNI 458 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1578 CAGCAGCTG
AspI 145 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACTTCGTC
AvaI 403 CTCGGG C^[CT]CG[GA]G
800 CTCGAG
BanI 1634 GGTGCC G^G[CT][GA]CC
1836 GGTGCC
BglII 2763 AGATCT A^GATCT
4098 AGATCT
BsaWI 770 TCCGGA [AT]^CCGG[AT]
2194 TCCGGA
BseAI 770 TCCGGA T^CCGGA
2194 TCCGGA
BsmI 833 GAATGC GAATGC(1/-1)
5067 GCATTC
BsoBI 403 CTCGGG C^[CT]CG[GA]G
800 CTCGAG
Bsp1407I 1519 TGTACA T^GTACA
4251 TGTACA
BspEI 770 TCCGGA T^CCGGA
2194 TCCGGA
BspHI 988 TCATGA T^CATGA
4286 TCATGA
BspLU11I 2964 ACATGT A^CATGT
5096 ACATGT
BspMI 1198 ACCTGC ACCTGC(4/8)
2491 ACCTGC
BsrGI 1519 TGTACA T^GTACA
4251 TGTACA
BssSI 446 CACGAG CACGAG(-5/-1)
3877 CTCGTG
BstXI 2971 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4120 CCAGATATGTGG
DraII 318 GGGGCCT [GA]G^G[ACGT]CC[CT]
1362 AGGACCT
DsaI 1840 CCATGG C^C[GA][CT]GG
3807 CCATGG
EaeI 10 TGGCCG [CT]^GGCC[GA]
4511 TGGCCA
EcoO109I 318 GGGGCCT [GA]G^G[ACGT]CC[CT]
1362 AGGACCT
EcoRV 806 GATATC GAT^ATC
972 GATATC
EcoT22I 4847 ATGCAT ATGCA^T
4916 ATGCAT
FauI 3331 CCCGC CCCGC(4/6)
3589 GCGGG
MroI 770 TCCGGA T^CCGGA
2194 TCCGGA
NcoI 1840 CCATGG C^CATGG
3807 CCATGG
NsiI 4847 ATGCAT ATGCA^T
4916 ATGCAT
PflFI 145 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACTTCGTC
PstI 1200 CTGCAG CTGCA^G
3111 CTGCAG
RcaI 988 TCATGA T^CATGA
4286 TCATGA
SapI 2775 GAAGAGC GCTCTTC(1/4)
913 GCTCTTC
SphI 880 GCATGC GCATG^C
922 GCATGC
SspBI 1519 TGTACA T^GTACA
4251 TGTACA
Tth111I 145 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACTTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AocI ApoI
Asp700I AspHI AvaII BanII BbvI
BcnI BfaI BfrI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstAPI BstBI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI Eco81I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI NspV PleI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SfuI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI