##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AscI AseI AsnI AvrII BbeI
BclI BfrI BlnI BseAI Bsp1407I
BspEI BsrGI BssHII Bst1107I BstZ17I
CpoI DraI EcoRI EcoRV EcoT22I
FbaI FseI HpaI KasI KspI
MfeI MluI MroI MunI NarI
NdeI NotI NsiI PacI PmeI
PshBI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SgrAI SpeI
Sse8387I SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 785 GGTACC G^GTACC
AcsI 3394 GAATTT [GA]^AATT[CT]
AgeI 4166 ACCGGT A^CCGGT
AhdI 605 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3394 GAATTT [GA]^AATT[CT]
Asp718I 785 GGTACC G^GTACC
AspEI 605 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2237 TGCGCA TGC^GCA
BamHI 4781 GGATCC G^GATCC
BcgI 117 CGAGCCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2561 GTATCC GTATCC(6/5)
BsmBI 2952 CGTCTC CGTCTC(1/5)
BspDI 712 ATCGAT AT^CGAT
BstBI 5698 TTCGAA TT^CGAA
BstEII 3895 GGTAACC G^GT[ACGT]ACC
BstPI 3895 GGTAACC G^GT[ACGT]ACC
ClaI 712 ATCGAT AT^CGAT
DraIII 1696 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 605 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3895 GGTAACC G^GT[ACGT]ACC
FspI 2237 TGCGCA TGC^GCA
KpnI 785 GGTACC GGTAC^C
NspV 5698 TTCGAA TT^CGAA
PaeR7I 326 CTCGAG C^TCGAG
PinAI 4166 ACCGGT A^CCGGT
PshAI 3131 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3047 CGATCG CGAT^CG
SexAI 4496 ACCTGGT A^CC[AT]GGT
SfuI 5698 TTCGAA TT^CGAA
SnaBI 1639 TACGTA TAC^GTA
SphI 4517 GCATGC GCATG^C
XcmI 167 CCATGTTAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 326 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1799 GTGCAC G^TGCAC
5462 GTGCAC
ApaI 2545 GGGCCC GGGCC^C
5530 GGGCCC
ApaLI 1799 GTGCAC G^TGCAC
5462 GTGCAC
AspI 2221 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3070 GACCTCGTC
BglI 4860 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5185 GCCTGCCAGGC
BglII 693 AGATCT A^GATCT
827 AGATCT
BsiWI 1641 CGTACG C^GTACG
2333 CGTACG
BspLU11I 2141 ACATGT A^CATGT
3746 ACATGT
BssSI 730 CACGAG CACGAG(-5/-1)
1315 CTCGTG
DrdI 1864 GACTCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5234 GACAGGCTGGTC
EagI 4319 CGGCCG C^GGCCG
4668 CGGCCG
EclXI 4319 CGGCCG C^GGCCG
4668 CGGCCG
Eco52I 4319 CGGCCG C^GGCCG
4668 CGGCCG
HindIII 2207 AAGCTT A^AGCTT
3586 AAGCTT
NaeI 2392 GCCGGC GCC^GGC
2617 GCCGGC
NgoMIV 2392 GCCGGC G^CCGGC
2617 GCCGGC
NheI 3022 GCTAGC G^CTAGC
4629 GCTAGC
NruI 513 TCGCGA TCG^CGA
1061 TCGCGA
PflFI 2221 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3070 GACCTCGTC
ScaI 552 AGTACT AGT^ACT
956 AGTACT
SmaI 3491 CCCGGG CCC^GGG
4410 CCCGGG
StuI 2685 AGGCCT AGG^CCT
5549 AGGCCT
Tsp509I 1684 AATT ^AATT
3395 AATT
Tth111I 2221 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3070 GACCTCGTC
XmaCI 3491 CCCGGG C^CCGGG
4410 CCCGGG
XmaI 3491 CCCGGG C^CCGGG
4410 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmnI