################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccIII AclI AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII AvrII BbeI BciVI BlnI BsaHI BseAI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HaeII Hin1I HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI Psp1406I RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI Sse8387I StuI SwaI Tth111I Van91I ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 733 CGCG CG^CG AflII 2861 CTTAAG C^TTAAG AhdI 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 5016 ATTAAT AT^TAAT AsnI 5016 ATTAAT AT^TAAT AspEI 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 236 GGATCC G^GATCC BbrPI 373 CACGTG CAC^GTG BbvCI 4689 CCTCAGC CCTCAGC(-5/-2) BcgI 3813 GCAAAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2861 CTTAAG C^TTAAG BglI 11 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 1017 CTGCAC GTGCAG(16/14) BsiEI 738 CGATCG CG[GA][CT]^CG BsmI 3216 GCATTC GAATGC(1/-1) Bsp1407I 2847 TGTACA T^GTACA BsrGI 2847 TGTACA T^GTACA BstUI 733 CGCG CG^CG CfoI 734 GCGC GCG^C DraII 4173 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 3628 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4342 CCTAGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4173 AGGCCCT [GA]G^G[ACGT]CC[CT] HgaI 3728 GACGC GACGC(5/10) HhaI 734 GCGC GCG^C HinP1I 734 GCGC G^CGC MfeI 5552 CAATTG C^AATTG MunI 5552 CAATTG C^AATTG MvnI 733 CGCG CG^CG PmaCI 373 CACGTG CAC^GTG PmlI 373 CACGTG CAC^GTG PshAI 3898 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5016 ATTAAT AT^TAAT PvuI 738 CGATCG CGAT^CG ScaI 5465 AGTACT AGT^ACT SfiI 10 GGCCAACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2847 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 4211 GGTACC G^GTACC 4484 GGTACC AocI 2767 CCTAAGG CC^T[ACGT]AGG 4206 CCTGAGG Asp718I 4211 GGTACC G^GTACC 4484 GGTACC AvaII 783 GGACC G^G[AT]CC 3163 GGTCC BclI 1568 TGATCA T^GATCA 1974 TGATCA BsaAI 373 CACGTG [CT]AC^GT[GA] 807 CACGTA BsaI 3608 GAGACC GGTCTC(1/5) 2700 GGTCTC BsrDI 2564 GCAATG GCAATG(2/0) 2645 GCAATG BssSI 3764 CACGAG CACGAG(-5/-1) 3965 CACGAG BstEII 1705 GGTGACC G^GT[ACGT]ACC 2734 GGTAACC BstPI 1705 GGTGACC G^GT[ACGT]ACC 2734 GGTAACC Bsu36I 2767 CCTAAGG CC^T[ACGT]AGG 4206 CCTGAGG Eco81I 2767 CCTAAGG CC^T[ACGT]AGG 4206 CCTGAGG EcoO65I 1705 GGTGACC G^GT[ACGT]ACC 2734 GGTAACC EcoRI 816 GAATTC G^AATTC 3719 GAATTC FbaI 1568 TGATCA T^GATCA 1974 TGATCA HincII 832 GTTGAC GT[CT]^[GA]AC 3470 GTTGAC HindII 832 GTTGAC GT[CT]^[GA]AC 3470 GTTGAC KpnI 4211 GGTACC GGTAC^C 4484 GGTACC MslI 1261 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3853 CATGATGGTG NcoI 165 CCATGG C^CATGG 4280 CCATGG PaeR7I 1762 CTCGAG C^TCGAG 3821 CTCGAG SacI 1221 GAGCTC GAGCT^C 3824 GAGCTC SapI 3884 GAAGAGC GCTCTTC(1/4) 3826 GCTCTTC SmaI 2423 CCCGGG CCC^GGG 3677 CCCGGG SpeI 1402 ACTAGT A^CTAGT 2408 ACTAGT XbaI 2491 TCTAGA T^CTAGA 3580 TCTAGA XhoI 1762 CTCGAG C^TCGAG 3821 CTCGAG XmaCI 2423 CCCGGG C^CCGGG 3677 CCCGGG XmaI 2423 CCCGGG C^CCGGG 3677 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI BalI BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI