##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AspI AviII AvrII BbeI BciVI
BlnI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII Hin1I HpaI KasI KspI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI Psp1406I RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI Sse8387I StuI SwaI Tth111I
Van91I
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 733 CGCG CG^CG
AflII 2861 CTTAAG C^TTAAG
AhdI 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5016 ATTAAT AT^TAAT
AsnI 5016 ATTAAT AT^TAAT
AspEI 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 236 GGATCC G^GATCC
BbrPI 373 CACGTG CAC^GTG
BbvCI 4689 CCTCAGC CCTCAGC(-5/-2)
BcgI 3813 GCAAAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2861 CTTAAG C^TTAAG
BglI 11 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1017 CTGCAC GTGCAG(16/14)
BsiEI 738 CGATCG CG[GA][CT]^CG
BsmI 3216 GCATTC GAATGC(1/-1)
Bsp1407I 2847 TGTACA T^GTACA
BsrGI 2847 TGTACA T^GTACA
BstUI 733 CGCG CG^CG
CfoI 734 GCGC GCG^C
DraII 4173 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 3628 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 355 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4342 CCTAGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4173 AGGCCCT [GA]G^G[ACGT]CC[CT]
HgaI 3728 GACGC GACGC(5/10)
HhaI 734 GCGC GCG^C
HinP1I 734 GCGC G^CGC
MfeI 5552 CAATTG C^AATTG
MunI 5552 CAATTG C^AATTG
MvnI 733 CGCG CG^CG
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
PshAI 3898 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5016 ATTAAT AT^TAAT
PvuI 738 CGATCG CGAT^CG
ScaI 5465 AGTACT AGT^ACT
SfiI 10 GGCCAACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2847 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 4211 GGTACC G^GTACC
4484 GGTACC
AocI 2767 CCTAAGG CC^T[ACGT]AGG
4206 CCTGAGG
Asp718I 4211 GGTACC G^GTACC
4484 GGTACC
AvaII 783 GGACC G^G[AT]CC
3163 GGTCC
BclI 1568 TGATCA T^GATCA
1974 TGATCA
BsaAI 373 CACGTG [CT]AC^GT[GA]
807 CACGTA
BsaI 3608 GAGACC GGTCTC(1/5)
2700 GGTCTC
BsrDI 2564 GCAATG GCAATG(2/0)
2645 GCAATG
BssSI 3764 CACGAG CACGAG(-5/-1)
3965 CACGAG
BstEII 1705 GGTGACC G^GT[ACGT]ACC
2734 GGTAACC
BstPI 1705 GGTGACC G^GT[ACGT]ACC
2734 GGTAACC
Bsu36I 2767 CCTAAGG CC^T[ACGT]AGG
4206 CCTGAGG
Eco81I 2767 CCTAAGG CC^T[ACGT]AGG
4206 CCTGAGG
EcoO65I 1705 GGTGACC G^GT[ACGT]ACC
2734 GGTAACC
EcoRI 816 GAATTC G^AATTC
3719 GAATTC
FbaI 1568 TGATCA T^GATCA
1974 TGATCA
HincII 832 GTTGAC GT[CT]^[GA]AC
3470 GTTGAC
HindII 832 GTTGAC GT[CT]^[GA]AC
3470 GTTGAC
KpnI 4211 GGTACC GGTAC^C
4484 GGTACC
MslI 1261 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3853 CATGATGGTG
NcoI 165 CCATGG C^CATGG
4280 CCATGG
PaeR7I 1762 CTCGAG C^TCGAG
3821 CTCGAG
SacI 1221 GAGCTC GAGCT^C
3824 GAGCTC
SapI 3884 GAAGAGC GCTCTTC(1/4)
3826 GCTCTTC
SmaI 2423 CCCGGG CCC^GGG
3677 CCCGGG
SpeI 1402 ACTAGT A^CTAGT
2408 ACTAGT
XbaI 2491 TCTAGA T^CTAGA
3580 TCTAGA
XhoI 1762 CTCGAG C^TCGAG
3821 CTCGAG
XmaCI 2423 CCCGGG C^CCGGG
3677 CCCGGG
XmaI 2423 CCCGGG C^CCGGG
3677 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI