################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AclI Aor51HI AscI AseI AsnI BbrPI BciVI BsiWI BssHII BstBI CpoI Eco47III EcoT22I FseI HindIII HpaI KspI MluI NaeI NcoI NgoMIV NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SfuI SgrAI SpeI SwaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 4341 GACGTC GACGT^C AccII 2590 CGCG CG^CG AccIII 2568 TCCGGA T^CCGGA AflIII 2580 ACATGT A^C[GA][CT]GT AgeI 1821 ACCGGT A^CCGGT AhdI 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2663 GTGCAC G^TGCAC ApaLI 2663 GTGCAC G^TGCAC Asp700I 2279 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4638 TGCGCA TGC^GCA AvrII 1693 CCTAGG C^CTAGG BbeI 721 GGCGCC GGCGC^C BlnI 1693 CCTAGG C^CTAGG BlpI 1257 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1257 GCTGAGC GC^T[ACGT]AGC BseAI 2568 TCCGGA T^CCGGA BsiEI 5240 CGGCCG CG[GA][CT]^CG BspEI 2568 TCCGGA T^CCGGA BspLU11I 2580 ACATGT A^CATGT Bst1107I 1918 GTATAC GTA^TAC BstEII 4109 GGTGACC G^GT[ACGT]ACC BstPI 4109 GGTGACC G^GT[ACGT]ACC BstUI 2590 CGCG CG^CG BstZ17I 1918 GTATAC GTA^TAC CelII 1257 GCTGAGC GC^T[ACGT]AGC DsaI 5904 CCGTGG C^C[GA][CT]GG EagI 5240 CGGCCG C^GGCCG Eam1105I 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 5240 CGGCCG C^GGCCG Eco52I 5240 CGGCCG C^GGCCG EcoO65I 4109 GGTGACC G^GT[ACGT]ACC EcoRV 4904 GATATC GAT^ATC FspI 4638 TGCGCA TGC^GCA KasI 721 GGCGCC G^GCGCC MfeI 1450 CAATTG C^AATTG MroI 2568 TCCGGA T^CCGGA MunI 1450 CAATTG C^AATTG MvnI 2590 CGCG CG^CG NarI 721 GGCGCC GG^CGCC NheI 4246 GCTAGC G^CTAGC PinAI 1821 ACCGGT A^CCGGT SexAI 1204 ACCTGGT A^CC[AT]GGT SfiI 135 GGCCTTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 721 GGCGCC GGC^GCC SmaI 3183 CCCGGG CCC^GGG SnaBI 393 TACGTA TAC^GTA SphI 3178 GCATGC GCATG^C SspI 5876 AATATT AAT^ATT XmaCI 3183 CCCGGG C^CCGGG XmaI 3183 CCCGGG C^CCGGG XmnI 2279 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2220 GGTACC G^GTACC 4286 GGTACC AccI 1918 GTATAC GT^[AC][GT]AC 5000 GTAGAC AflII 2381 CTTAAG C^TTAAG 4190 CTTAAG AocI 1924 CCTGAGG CC^T[ACGT]AGG 5315 CCTGAGG ApaI 1246 GGGCCC GGGCC^C 4484 GGGCCC Asp718I 2220 GGTACC G^GTACC 4286 GGTACC BamHI 338 GGATCC G^GATCC 4674 GGATCC BbsI 2438 GTCTTC GAAGAC(2/6) 4614 GTCTTC BfrI 2381 CTTAAG C^TTAAG 4190 CTTAAG BpuAI 2438 GTCTTC GAAGAC(2/6) 4614 GTCTTC BsaAI 393 TACGTA [CT]AC^GT[GA] 1146 CACGTA BsaBI 906 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5996 GATTTCAATC BsaWI 1821 ACCGGT [AT]^CCGG[AT] 2568 TCCGGA BsmI 2887 GAATGC GAATGC(1/-1) 5889 GCATTC BspDI 3170 ATCGAT AT^CGAT 5456 ATCGAT Bsu36I 1924 CCTGAGG CC^T[ACGT]AGG 5315 CCTGAGG ClaI 3170 ATCGAT AT^CGAT 5456 ATCGAT DraI 2842 TTTAAA TTT^AAA 5042 TTTAAA DrdI 492 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 580 GACTTGTAGGTC Eco81I 1924 CCTGAGG CC^T[ACGT]AGG 5315 CCTGAGG EcoRI 1649 GAATTC G^AATTC 6026 GAATTC FauI 50 CCCGC CCCGC(4/6) 2808 CCCGC HaeII 721 GGCGCC [GA]GCGC^[CT] 5646 AGCGCC HincII 1177 GTTGAC GT[CT]^[GA]AC 3218 GTTGAC HindII 1177 GTTGAC GT[CT]^[GA]AC 3218 GTTGAC KpnI 2220 GGTACC GGTAC^C 4286 GGTACC MamI 906 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5996 GATTTCAATC NdeI 739 CATATG CA^TATG 3160 CATATG PaeR7I 2708 CTCGAG C^TCGAG 4419 CTCGAG PflMI 2641 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2752 CCAGGACCTGG SacI 86 GAGCTC GAGCT^C 618 GAGCTC SbfI 3437 CCTGCAGG CCTGCA^GG 5261 CCTGCAGG ScaI 2685 AGTACT AGT^ACT 3330 AGTACT Sse8387I 3437 CCTGCAGG CCTGCA^GG 5261 CCTGCAGG Van91I 2641 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2752 CCAGGACCTGG XbaI 823 TCTAGA T^CTAGA 5395 TCTAGA XhoI 2708 CTCGAG C^TCGAG 4419 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcgI BclI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII