##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AclI Aor51HI AscI AseI AsnI
BbrPI BciVI BsiWI BssHII BstBI
CpoI Eco47III EcoT22I FseI HindIII
HpaI KspI MluI NaeI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SfuI SgrAI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 4341 GACGTC GACGT^C
AccII 2590 CGCG CG^CG
AccIII 2568 TCCGGA T^CCGGA
AflIII 2580 ACATGT A^C[GA][CT]GT
AgeI 1821 ACCGGT A^CCGGT
AhdI 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2663 GTGCAC G^TGCAC
ApaLI 2663 GTGCAC G^TGCAC
Asp700I 2279 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4638 TGCGCA TGC^GCA
AvrII 1693 CCTAGG C^CTAGG
BbeI 721 GGCGCC GGCGC^C
BlnI 1693 CCTAGG C^CTAGG
BlpI 1257 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1257 GCTGAGC GC^T[ACGT]AGC
BseAI 2568 TCCGGA T^CCGGA
BsiEI 5240 CGGCCG CG[GA][CT]^CG
BspEI 2568 TCCGGA T^CCGGA
BspLU11I 2580 ACATGT A^CATGT
Bst1107I 1918 GTATAC GTA^TAC
BstEII 4109 GGTGACC G^GT[ACGT]ACC
BstPI 4109 GGTGACC G^GT[ACGT]ACC
BstUI 2590 CGCG CG^CG
BstZ17I 1918 GTATAC GTA^TAC
CelII 1257 GCTGAGC GC^T[ACGT]AGC
DsaI 5904 CCGTGG C^C[GA][CT]GG
EagI 5240 CGGCCG C^GGCCG
Eam1105I 3527 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5240 CGGCCG C^GGCCG
Eco52I 5240 CGGCCG C^GGCCG
EcoO65I 4109 GGTGACC G^GT[ACGT]ACC
EcoRV 4904 GATATC GAT^ATC
FspI 4638 TGCGCA TGC^GCA
KasI 721 GGCGCC G^GCGCC
MfeI 1450 CAATTG C^AATTG
MroI 2568 TCCGGA T^CCGGA
MunI 1450 CAATTG C^AATTG
MvnI 2590 CGCG CG^CG
NarI 721 GGCGCC GG^CGCC
NheI 4246 GCTAGC G^CTAGC
PinAI 1821 ACCGGT A^CCGGT
SexAI 1204 ACCTGGT A^CC[AT]GGT
SfiI 135 GGCCTTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 721 GGCGCC GGC^GCC
SmaI 3183 CCCGGG CCC^GGG
SnaBI 393 TACGTA TAC^GTA
SphI 3178 GCATGC GCATG^C
SspI 5876 AATATT AAT^ATT
XmaCI 3183 CCCGGG C^CCGGG
XmaI 3183 CCCGGG C^CCGGG
XmnI 2279 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2220 GGTACC G^GTACC
4286 GGTACC
AccI 1918 GTATAC GT^[AC][GT]AC
5000 GTAGAC
AflII 2381 CTTAAG C^TTAAG
4190 CTTAAG
AocI 1924 CCTGAGG CC^T[ACGT]AGG
5315 CCTGAGG
ApaI 1246 GGGCCC GGGCC^C
4484 GGGCCC
Asp718I 2220 GGTACC G^GTACC
4286 GGTACC
BamHI 338 GGATCC G^GATCC
4674 GGATCC
BbsI 2438 GTCTTC GAAGAC(2/6)
4614 GTCTTC
BfrI 2381 CTTAAG C^TTAAG
4190 CTTAAG
BpuAI 2438 GTCTTC GAAGAC(2/6)
4614 GTCTTC
BsaAI 393 TACGTA [CT]AC^GT[GA]
1146 CACGTA
BsaBI 906 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5996 GATTTCAATC
BsaWI 1821 ACCGGT [AT]^CCGG[AT]
2568 TCCGGA
BsmI 2887 GAATGC GAATGC(1/-1)
5889 GCATTC
BspDI 3170 ATCGAT AT^CGAT
5456 ATCGAT
Bsu36I 1924 CCTGAGG CC^T[ACGT]AGG
5315 CCTGAGG
ClaI 3170 ATCGAT AT^CGAT
5456 ATCGAT
DraI 2842 TTTAAA TTT^AAA
5042 TTTAAA
DrdI 492 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
580 GACTTGTAGGTC
Eco81I 1924 CCTGAGG CC^T[ACGT]AGG
5315 CCTGAGG
EcoRI 1649 GAATTC G^AATTC
6026 GAATTC
FauI 50 CCCGC CCCGC(4/6)
2808 CCCGC
HaeII 721 GGCGCC [GA]GCGC^[CT]
5646 AGCGCC
HincII 1177 GTTGAC GT[CT]^[GA]AC
3218 GTTGAC
HindII 1177 GTTGAC GT[CT]^[GA]AC
3218 GTTGAC
KpnI 2220 GGTACC GGTAC^C
4286 GGTACC
MamI 906 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5996 GATTTCAATC
NdeI 739 CATATG CA^TATG
3160 CATATG
PaeR7I 2708 CTCGAG C^TCGAG
4419 CTCGAG
PflMI 2641 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2752 CCAGGACCTGG
SacI 86 GAGCTC GAGCT^C
618 GAGCTC
SbfI 3437 CCTGCAGG CCTGCA^GG
5261 CCTGCAGG
ScaI 2685 AGTACT AGT^ACT
3330 AGTACT
Sse8387I 3437 CCTGCAGG CCTGCA^GG
5261 CCTGCAGG
Van91I 2641 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2752 CCAGGACCTGG
XbaI 823 TCTAGA T^CTAGA
5395 TCTAGA
XhoI 2708 CTCGAG C^TCGAG
4419 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BclI BcnI
BfaI BglI BglII BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII