##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AclI        Aor51HI     AscI        AseI        AsnI        
BbrPI       BciVI       BsiWI       BssHII      BstBI       
CpoI        Eco47III    EcoT22I     FseI        HindIII     
HpaI        KspI        MluI        NaeI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SfuI        SgrAI       SpeI        
SwaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        4341  GACGTC               GACGT^C
AccII        2590  CGCG                 CG^CG
AccIII       2568  TCCGGA               T^CCGGA
AflIII       2580  ACATGT               A^C[GA][CT]GT
AgeI         1821  ACCGGT               A^CCGGT
AhdI         3527  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2663  GTGCAC               G^TGCAC
ApaLI        2663  GTGCAC               G^TGCAC
Asp700I      2279  GAACTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3527  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4638  TGCGCA               TGC^GCA
AvrII        1693  CCTAGG               C^CTAGG
BbeI          721  GGCGCC               GGCGC^C
BlnI         1693  CCTAGG               C^CTAGG
BlpI         1257  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1257  GCTGAGC              GC^T[ACGT]AGC
BseAI        2568  TCCGGA               T^CCGGA
BsiEI        5240  CGGCCG               CG[GA][CT]^CG
BspEI        2568  TCCGGA               T^CCGGA
BspLU11I     2580  ACATGT               A^CATGT
Bst1107I     1918  GTATAC               GTA^TAC
BstEII       4109  GGTGACC              G^GT[ACGT]ACC
BstPI        4109  GGTGACC              G^GT[ACGT]ACC
BstUI        2590  CGCG                 CG^CG
BstZ17I      1918  GTATAC               GTA^TAC
CelII        1257  GCTGAGC              GC^T[ACGT]AGC
DsaI         5904  CCGTGG               C^C[GA][CT]GG
EagI         5240  CGGCCG               C^GGCCG
Eam1105I     3527  GACTACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5240  CGGCCG               C^GGCCG
Eco52I       5240  CGGCCG               C^GGCCG
EcoO65I      4109  GGTGACC              G^GT[ACGT]ACC
EcoRV        4904  GATATC               GAT^ATC
FspI         4638  TGCGCA               TGC^GCA
KasI          721  GGCGCC               G^GCGCC
MfeI         1450  CAATTG               C^AATTG
MroI         2568  TCCGGA               T^CCGGA
MunI         1450  CAATTG               C^AATTG
MvnI         2590  CGCG                 CG^CG
NarI          721  GGCGCC               GG^CGCC
NheI         4246  GCTAGC               G^CTAGC
PinAI        1821  ACCGGT               A^CCGGT
SexAI        1204  ACCTGGT              A^CC[AT]GGT
SfiI          135  GGCCTTAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          721  GGCGCC               GGC^GCC
SmaI         3183  CCCGGG               CCC^GGG
SnaBI         393  TACGTA               TAC^GTA
SphI         3178  GCATGC               GCATG^C
SspI         5876  AATATT               AAT^ATT
XmaCI        3183  CCCGGG               C^CCGGG
XmaI         3183  CCCGGG               C^CCGGG
XmnI         2279  GAACTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       2220  GGTACC               G^GTACC
             4286  GGTACC              
AccI         1918  GTATAC               GT^[AC][GT]AC
             5000  GTAGAC              
AflII        2381  CTTAAG               C^TTAAG
             4190  CTTAAG              
AocI         1924  CCTGAGG              CC^T[ACGT]AGG
             5315  CCTGAGG             
ApaI         1246  GGGCCC               GGGCC^C
             4484  GGGCCC              
Asp718I      2220  GGTACC               G^GTACC
             4286  GGTACC              
BamHI         338  GGATCC               G^GATCC
             4674  GGATCC              
BbsI         2438  GTCTTC               GAAGAC(2/6)
             4614  GTCTTC              
BfrI         2381  CTTAAG               C^TTAAG
             4190  CTTAAG              
BpuAI        2438  GTCTTC               GAAGAC(2/6)
             4614  GTCTTC              
BsaAI         393  TACGTA               [CT]AC^GT[GA]
             1146  CACGTA              
BsaBI         906  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5996  GATTTCAATC          
BsaWI        1821  ACCGGT               [AT]^CCGG[AT]
             2568  TCCGGA              
BsmI         2887  GAATGC               GAATGC(1/-1)
             5889  GCATTC              
BspDI        3170  ATCGAT               AT^CGAT
             5456  ATCGAT              
Bsu36I       1924  CCTGAGG              CC^T[ACGT]AGG
             5315  CCTGAGG             
ClaI         3170  ATCGAT               AT^CGAT
             5456  ATCGAT              
DraI         2842  TTTAAA               TTT^AAA
             5042  TTTAAA              
DrdI          492  GACACAGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              580  GACTTGTAGGTC        
Eco81I       1924  CCTGAGG              CC^T[ACGT]AGG
             5315  CCTGAGG             
EcoRI        1649  GAATTC               G^AATTC
             6026  GAATTC              
FauI           50  CCCGC                CCCGC(4/6)
             2808  CCCGC               
HaeII         721  GGCGCC               [GA]GCGC^[CT]
             5646  AGCGCC              
HincII       1177  GTTGAC               GT[CT]^[GA]AC
             3218  GTTGAC              
HindII       1177  GTTGAC               GT[CT]^[GA]AC
             3218  GTTGAC              
KpnI         2220  GGTACC               GGTAC^C
             4286  GGTACC              
MamI          906  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5996  GATTTCAATC          
NdeI          739  CATATG               CA^TATG
             3160  CATATG              
PaeR7I       2708  CTCGAG               C^TCGAG
             4419  CTCGAG              
PflMI        2641  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2752  CCAGGACCTGG         
SacI           86  GAGCTC               GAGCT^C
              618  GAGCTC              
SbfI         3437  CCTGCAGG             CCTGCA^GG
             5261  CCTGCAGG            
ScaI         2685  AGTACT               AGT^ACT
             3330  AGTACT              
Sse8387I     3437  CCTGCAGG             CCTGCA^GG
             5261  CCTGCAGG            
Van91I       2641  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2752  CCAGGACCTGG         
XbaI          823  TCTAGA               T^CTAGA
             5395  TCTAGA              
XhoI         2708  CTCGAG               C^TCGAG
             4419  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcgI        BclI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII