##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaI
ApaLI AscI Asp700I AviII BbeI
BcgI BglI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DrdI EcoO65I
EcoT22I FseI FspI KasI KspI
MfeI MluI MroI MunI NarI
NotI NsiI PacI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1085 GACGTC GACGT^C
Acc65I 3343 GGTACC G^GTACC
AcyI 1085 GACGTC G[GA]^CG[CT]C
AflII 1394 CTTAAG C^TTAAG
AflIII 3981 ACACGT A^C[GA][CT]GT
Aor51HI 4641 AGCGCT AGC^GCT
AseI 1547 ATTAAT AT^TAAT
AsnI 1547 ATTAAT AT^TAAT
Asp718I 3343 GGTACC G^GTACC
AvrII 3130 CCTAGG C^CTAGG
BamHI 540 GGATCC G^GATCC
BfrI 1394 CTTAAG C^TTAAG
BlnI 3130 CCTAGG C^CTAGG
BsaHI 1085 GACGTC G[GA]^CG[CT]C
BsaWI 537 ACCGGA [AT]^CCGG[AT]
BsmBI 2067 CGTCTC CGTCTC(1/5)
BsmI 4857 GCATTC GAATGC(1/-1)
BspHI 1798 TCATGA T^CATGA
BstAPI 1606 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 5200 TTTAAA TTT^AAA
Eco47III 4641 AGCGCT AGC^GCT
EcoRI 3404 GAATTC G^AATTC
Hin1I 1085 GACGTC G[GA]^CG[CT]C
HpaI 24 GTTAAC GTT^AAC
KpnI 3343 GGTACC GGTAC^C
NheI 4891 GCTAGC G^CTAGC
NruI 1275 TCGCGA TCG^CGA
PshBI 1547 ATTAAT AT^TAAT
RcaI 1798 TCATGA T^CATGA
SspI 4818 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2186 GTAGAC GT^[AC][GT]AC
4410 GTCTAC
AhdI 2765 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4485 GACTTGTAGTC
AspEI 2765 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4485 GACTTGTAGTC
BbrPI 2547 CACGTG CAC^GTG
3982 CACGTG
BciVI 1726 GTATCC GTATCC(6/5)
4838 GTATCC
BsaAI 2547 CACGTG [CT]AC^GT[GA]
3982 CACGTG
BsaBI 2731 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5111 GATTTGCATC
BsiEI 1400 CGGCCG CG[GA][CT]^CG
3276 CGGCCG
BspMI 2190 ACCTGC ACCTGC(4/8)
2493 ACCTGC
BsrBI 2269 GAGCGG CCGCTC(-3/-3)
2780 GAGCGG
BsrFI 1751 GCCGGC [GA]^CCGG[CT]
2846 GCCGGC
BssSI 2507 CACGAG CACGAG(-5/-1)
3741 CACGAG
BstBI 471 TTCGAA TT^CGAA
2577 TTCGAA
Cfr10I 1751 GCCGGC [GA]^CCGG[CT]
2846 GCCGGC
EagI 1400 CGGCCG C^GGCCG
3276 CGGCCG
Eam1105I 2765 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4485 GACTTGTAGTC
EclXI 1400 CGGCCG C^GGCCG
3276 CGGCCG
Eco52I 1400 CGGCCG C^GGCCG
3276 CGGCCG
EcoNI 544 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3991 CCTGTGCCAGG
HindIII 467 AAGCTT A^AGCTT
5389 AAGCTT
MamI 2731 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5111 GATTTGCATC
NaeI 1751 GCCGGC GCC^GGC
2846 GCCGGC
NgoMIV 1751 GCCGGC G^CCGGC
2846 GCCGGC
NspV 471 TTCGAA TT^CGAA
2577 TTCGAA
PaeR7I 1499 CTCGAG C^TCGAG
2216 CTCGAG
PflMI 4438 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4701 CCATGGAGTGG
PmaCI 2547 CACGTG CAC^GTG
3982 CACGTG
PmlI 2547 CACGTG CAC^GTG
3982 CACGTG
PshAI 343 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3412 GACTAAAGTC
ScaI 1122 AGTACT AGT^ACT
3593 AGTACT
SexAI 636 ACCAGGT A^CC[AT]GGT
3070 ACCTGGT
SfuI 471 TTCGAA TT^CGAA
2577 TTCGAA
Van91I 4438 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4701 CCATGGAGTGG
XbaI 313 TCTAGA T^CTAGA
1876 TCTAGA
XhoI 1499 CTCGAG C^TCGAG
2216 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII