##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AgeI Alw44I AocI ApaLI
AscI AseI AsnI AvrII BclI
BglII BlnI BsiWI BspHI BssHII
BstBI Bsu36I CpoI DraI Eco81I
EcoRV EcoT22I FbaI FseI HpaI
KspI MfeI MluI MunI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2779 GACGTC GACGT^C
AflII 16 CTTAAG C^TTAAG
AflIII 3296 ACATGT A^C[GA][CT]GT
AhdI 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3914 AGCGCT AGC^GCT
AspEI 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3181 CACGTG CAC^GTG
BciVI 2094 GTATCC GTATCC(6/5)
BfrI 16 CTTAAG C^TTAAG
BsaBI 1867 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 3989 ATCGAT AT^CGAT
BspLU11I 3296 ACATGT A^CATGT
BssSI 2576 CTCGTG CACGAG(-5/-1)
Bst1107I 3053 GTATAC GTA^TAC
BstEII 3958 GGTCACC G^GT[ACGT]ACC
BstPI 3958 GGTCACC G^GT[ACGT]ACC
BstZ17I 3053 GTATAC GTA^TAC
ClaI 3989 ATCGAT AT^CGAT
EagI 176 CGGCCG C^GGCCG
Eam1105I 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 176 CGGCCG C^GGCCG
Eco47III 3914 AGCGCT AGC^GCT
Eco52I 176 CGGCCG C^GGCCG
EcoNI 2752 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3958 GGTCACC G^GT[ACGT]ACC
HindIII 830 AAGCTT A^AGCTT
MamI 1867 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3645 GCCGGC GCC^GGC
NdeI 821 CATATG CA^TATG
NgoMIV 3645 GCCGGC G^CCGGC
NheI 145 GCTAGC G^CTAGC
PmaCI 3181 CACGTG CAC^GTG
PmlI 3181 CACGTG CAC^GTG
PshAI 2547 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3589 GAGCTC GAGCT^C
SapI 111 GCTCTTC GCTCTTC(1/4)
ScaI 1689 AGTACT AGT^ACT
SexAI 4443 ACCAGGT A^CC[AT]GGT
SnaBI 1575 TACGTA TAC^GTA
SspI 4988 AATATT AAT^ATT
XbaI 2047 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 69 GGTACC G^GTACC
4124 GGTACC
AccIII 2271 TCCGGA T^CCGGA
4146 TCCGGA
Asp700I 1528 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2524 GAACCAGTTC
Asp718I 69 GGTACC G^GTACC
4124 GGTACC
AspI 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
AviII 1218 TGCGCA TGC^GCA
2121 TGCGCA
BamHI 438 GGATCC G^GATCC
4864 GGATCC
BbeI 294 GGCGCC GGCGC^C
1623 GGCGCC
BbsI 1955 GAAGAC GAAGAC(2/6)
2857 GAAGAC
BlpI 1 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
Bpu1102I 1 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
BpuAI 1955 GAAGAC GAAGAC(2/6)
2857 GAAGAC
BsaAI 1575 TACGTA [CT]AC^GT[GA]
3181 CACGTG
BsaI 1139 GAGACC GGTCTC(1/5)
3784 GGTCTC
BseAI 2271 TCCGGA T^CCGGA
4146 TCCGGA
BsiEI 176 CGGCCG CG[GA][CT]^CG
257 CGACCG
BsmBI 2781 CGTCTC CGTCTC(1/5)
3920 GAGACG
BsmI 2951 GAATGC GAATGC(1/-1)
4664 GAATGC
Bsp1407I 1353 TGTACA T^GTACA
4155 TGTACA
BspEI 2271 TCCGGA T^CCGGA
4146 TCCGGA
BsrBI 109 CCGCTC CCGCTC(-3/-3)
4382 CCGCTC
BsrFI 335 ACCGGC [GA]^CCGG[CT]
3645 GCCGGC
BsrGI 1353 TGTACA T^GTACA
4155 TGTACA
BstAPI 1250 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4480 GCAGTGCCTGC
CelII 1 GCTGAGC GC^T[ACGT]AGC
1674 GCTCAGC
Cfr10I 335 ACCGGC [GA]^CCGG[CT]
3645 GCCGGC
DrdI 2438 GACACAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2779 GACGTCTCTGTC
EcoRI 992 GAATTC G^AATTC
1291 GAATTC
FspI 1218 TGCGCA TGC^GCA
2121 TGCGCA
KasI 294 GGCGCC G^GCGCC
1623 GGCGCC
KpnI 69 GGTACC GGTAC^C
4124 GGTACC
MroI 2271 TCCGGA T^CCGGA
4146 TCCGGA
NarI 294 GGCGCC GG^CGCC
1623 GGCGCC
PflFI 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
SfoI 294 GGCGCC GGC^GCC
1623 GGCGCC
SmaI 4681 CCCGGG CCC^GGG
4935 CCCGGG
SphI 3247 GCATGC GCATG^C
3426 GCATGC
SspBI 1353 TGTACA T^GTACA
4155 TGTACA
Tth111I 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
XmaCI 4681 CCCGGG C^CCGGG
4935 CCCGGG
XmaI 4681 CCCGGG C^CCGGG
4935 CCCGGG
XmnI 1528 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2524 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII