################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AclI AgeI Alw44I AocI ApaLI AscI AseI AsnI AvrII BclI BglII BlnI BsiWI BspHI BssHII BstBI Bsu36I CpoI DraI Eco81I EcoRV EcoT22I FbaI FseI HpaI KspI MfeI MluI MunI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 2779 GACGTC GACGT^C AflII 16 CTTAAG C^TTAAG AflIII 3296 ACATGT A^C[GA][CT]GT AhdI 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3914 AGCGCT AGC^GCT AspEI 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3181 CACGTG CAC^GTG BciVI 2094 GTATCC GTATCC(6/5) BfrI 16 CTTAAG C^TTAAG BsaBI 1867 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 3989 ATCGAT AT^CGAT BspLU11I 3296 ACATGT A^CATGT BssSI 2576 CTCGTG CACGAG(-5/-1) Bst1107I 3053 GTATAC GTA^TAC BstEII 3958 GGTCACC G^GT[ACGT]ACC BstPI 3958 GGTCACC G^GT[ACGT]ACC BstZ17I 3053 GTATAC GTA^TAC ClaI 3989 ATCGAT AT^CGAT EagI 176 CGGCCG C^GGCCG Eam1105I 2774 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 176 CGGCCG C^GGCCG Eco47III 3914 AGCGCT AGC^GCT Eco52I 176 CGGCCG C^GGCCG EcoNI 2752 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3958 GGTCACC G^GT[ACGT]ACC HindIII 830 AAGCTT A^AGCTT MamI 1867 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3645 GCCGGC GCC^GGC NdeI 821 CATATG CA^TATG NgoMIV 3645 GCCGGC G^CCGGC NheI 145 GCTAGC G^CTAGC PmaCI 3181 CACGTG CAC^GTG PmlI 3181 CACGTG CAC^GTG PshAI 2547 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3589 GAGCTC GAGCT^C SapI 111 GCTCTTC GCTCTTC(1/4) ScaI 1689 AGTACT AGT^ACT SexAI 4443 ACCAGGT A^CC[AT]GGT SnaBI 1575 TACGTA TAC^GTA SspI 4988 AATATT AAT^ATT XbaI 2047 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 69 GGTACC G^GTACC 4124 GGTACC AccIII 2271 TCCGGA T^CCGGA 4146 TCCGGA Asp700I 1528 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2524 GAACCAGTTC Asp718I 69 GGTACC G^GTACC 4124 GGTACC AspI 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4589 GACCCTGTC AviII 1218 TGCGCA TGC^GCA 2121 TGCGCA BamHI 438 GGATCC G^GATCC 4864 GGATCC BbeI 294 GGCGCC GGCGC^C 1623 GGCGCC BbsI 1955 GAAGAC GAAGAC(2/6) 2857 GAAGAC BlpI 1 GCTGAGC GC^T[ACGT]AGC 1674 GCTCAGC Bpu1102I 1 GCTGAGC GC^T[ACGT]AGC 1674 GCTCAGC BpuAI 1955 GAAGAC GAAGAC(2/6) 2857 GAAGAC BsaAI 1575 TACGTA [CT]AC^GT[GA] 3181 CACGTG BsaI 1139 GAGACC GGTCTC(1/5) 3784 GGTCTC BseAI 2271 TCCGGA T^CCGGA 4146 TCCGGA BsiEI 176 CGGCCG CG[GA][CT]^CG 257 CGACCG BsmBI 2781 CGTCTC CGTCTC(1/5) 3920 GAGACG BsmI 2951 GAATGC GAATGC(1/-1) 4664 GAATGC Bsp1407I 1353 TGTACA T^GTACA 4155 TGTACA BspEI 2271 TCCGGA T^CCGGA 4146 TCCGGA BsrBI 109 CCGCTC CCGCTC(-3/-3) 4382 CCGCTC BsrFI 335 ACCGGC [GA]^CCGG[CT] 3645 GCCGGC BsrGI 1353 TGTACA T^GTACA 4155 TGTACA BstAPI 1250 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4480 GCAGTGCCTGC CelII 1 GCTGAGC GC^T[ACGT]AGC 1674 GCTCAGC Cfr10I 335 ACCGGC [GA]^CCGG[CT] 3645 GCCGGC DrdI 2438 GACACAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2779 GACGTCTCTGTC EcoRI 992 GAATTC G^AATTC 1291 GAATTC FspI 1218 TGCGCA TGC^GCA 2121 TGCGCA KasI 294 GGCGCC G^GCGCC 1623 GGCGCC KpnI 69 GGTACC GGTAC^C 4124 GGTACC MroI 2271 TCCGGA T^CCGGA 4146 TCCGGA NarI 294 GGCGCC GG^CGCC 1623 GGCGCC PflFI 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4589 GACCCTGTC SfoI 294 GGCGCC GGC^GCC 1623 GGCGCC SmaI 4681 CCCGGG CCC^GGG 4935 CCCGGG SphI 3247 GCATGC GCATG^C 3426 GCATGC SspBI 1353 TGTACA T^GTACA 4155 TGTACA Tth111I 46 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4589 GACCCTGTC XmaCI 4681 CCCGGG C^CCGGG 4935 CCCGGG XmaI 4681 CCCGGG C^CCGGG 4935 CCCGGG XmnI 1528 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2524 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII