##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BfrI BsiEI BsiWI
BspDI BspHI BspLU11I BssHII Bst1107I
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI MfeI MluI
MunI NaeI NgoMIV NotI NsiI
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3459 GACGTC GACGT^C
AccIII 3760 TCCGGA T^CCGGA
AflIII 972 ACGTGT A^C[GA][CT]GT
Asp700I 37 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1108 CCTAGG C^CTAGG
BamHI 2360 GGATCC G^GATCC
BbeI 70 GGCGCC GGCGC^C
BbsI 1535 GAAGAC GAAGAC(2/6)
BbvCI 1529 GCTGAGG CCTCAGC(-5/-2)
BclI 2292 TGATCA T^GATCA
BglII 2697 AGATCT A^GATCT
BlnI 1108 CCTAGG C^CTAGG
BlpI 213 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC
BpuAI 1535 GAAGAC GAAGAC(2/6)
BsaBI 1934 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3760 TCCGGA T^CCGGA
Bsp1407I 2755 TGTACA T^GTACA
BspEI 3760 TCCGGA T^CCGGA
BsrFI 1230 ACCGGC [GA]^CCGG[CT]
BsrGI 2755 TGTACA T^GTACA
BstBI 4858 TTCGAA TT^CGAA
CelII 213 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1230 ACCGGC [GA]^CCGG[CT]
DraIII 2423 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 2292 TGATCA T^GATCA
KasI 70 GGCGCC G^GCGCC
KspI 333 CCGCGG CCGC^GG
MamI 1934 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3760 TCCGGA T^CCGGA
MslI 1780 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 70 GGCGCC GG^CGCC
NdeI 2247 CATATG CA^TATG
NruI 4761 TCGCGA TCG^CGA
NspV 4858 TTCGAA TT^CGAA
PshAI 1952 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2118 GAGCTC GAGCT^C
SacII 333 CCGCGG CCGC^GG
SapI 4160 GAAGAGC GCTCTTC(1/4)
SbfI 3707 CCTGCAGG CCTGCA^GG
ScaI 799 AGTACT AGT^ACT
SexAI 3536 ACCTGGT A^CC[AT]GGT
SfoI 70 GGCGCC GGC^GCC
SfuI 4858 TTCGAA TT^CGAA
Sse8387I 3707 CCTGCAGG CCTGCA^GG
SspBI 2755 TGTACA T^GTACA
XcmI 1515 CCAGACACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 37 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1959 GTCTAC GT^[AC][GT]AC
4289 GTAGAC
Alw44I 673 GTGCAC G^TGCAC
986 GTGCAC
AocI 1481 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
ApaLI 673 GTGCAC G^TGCAC
986 GTGCAC
AspI 1310 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2315 GACCGAGTC
BbrPI 3071 CACGTG CAC^GTG
3243 CACGTG
BcgI 1357 CGATATGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3710 GCAGGGCAGTCG
BciVI 3979 GGATAC GTATCC(6/5)
4625 GTATCC
BglI 192 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
998 GCCGTGGAGGC
BsaAI 3071 CACGTG [CT]AC^GT[GA]
3243 CACGTG
BsaI 1689 GAGACC GGTCTC(1/5)
1884 GGTCTC
BsmBI 3457 GAGACG CGTCTC(1/5)
3681 GAGACG
BsrBI 211 CCGCTC CCGCTC(-3/-3)
4775 GAGCGG
BstEII 394 GGTGACC G^GT[ACGT]ACC
1462 GGTCACC
BstPI 394 GGTGACC G^GT[ACGT]ACC
1462 GGTCACC
BstXI 2712 CCATGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3534 CCACCTGGTTGG
Bsu36I 1481 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
DraI 3014 TTTAAA TTT^AAA
4584 TTTAAA
Eco81I 1481 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
EcoO65I 394 GGTGACC G^GT[ACGT]ACC
1462 GGTCACC
HaeII 70 GGCGCC [GA]GCGC^[CT]
3253 GGCGCT
NcoI 152 CCATGG C^CATGG
918 CCATGG
NheI 488 GCTAGC G^CTAGC
3111 GCTAGC
NspI 777 GCATGT [GA]CATG^[CT]
1521 ACATGC
PflFI 1310 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2315 GACCGAGTC
PflMI 2422 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4252 CCAACAGCTGG
PmaCI 3071 CACGTG CAC^GTG
3243 CACGTG
PmlI 3071 CACGTG CAC^GTG
3243 CACGTG
SmaI 2436 CCCGGG CCC^GGG
3259 CCCGGG
SmlI 422 CTTGAG C^T[CT][GA]AG
1295 CTCAAG
Tth111I 1310 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2315 GACCGAGTC
Van91I 2422 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4252 CCAACAGCTGG
XmaCI 2436 CCCGGG C^CCGGG
3259 CCCGGG
XmaI 2436 CCCGGG C^CCGGG
3259 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII