##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AviII BciVI BsaBI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FseI FspI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SphI Sse8387I
SspI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1506 GACGTC GACGT^C
Acc65I 5802 GGTACC G^GTACC
Asp718I 5802 GGTACC G^GTACC
AvrII 3046 CCTAGG C^CTAGG
BamHI 3646 GGATCC G^GATCC
BbsI 5411 GAAGAC GAAGAC(2/6)
BcgI 4465 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 188 TGATCA T^GATCA
BlnI 3046 CCTAGG C^CTAGG
BlpI 4038 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4038 GCTCAGC GC^T[ACGT]AGC
BpuAI 5411 GAAGAC GAAGAC(2/6)
Bsp1407I 2056 TGTACA T^GTACA
BsrGI 2056 TGTACA T^GTACA
BssSI 264 CTCGTG CACGAG(-5/-1)
BstXI 1780 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4038 GCTCAGC GC^T[ACGT]AGC
DrdI 5364 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1943 GATATC GAT^ATC
FbaI 188 TGATCA T^GATCA
KpnI 5802 GGTACC GGTAC^C
NdeI 1138 CATATG CA^TATG
PshAI 1004 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 2226 GGCCAGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1458 CCCGGG CCC^GGG
SpeI 6047 ACTAGT A^CTAGT
SspBI 2056 TGTACA T^GTACA
SwaI 6496 ATTTAAAT ATTT^AAAT
XmaCI 1458 CCCGGG C^CCGGG
XmaI 1458 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2217 GTCTAC GT^[AC][GT]AC
6033 GTCTAC
AocI 69 CCTGAGG CC^T[ACGT]AGG
1155 CCTGAGG
ApaI 3468 GGGCCC GGGCC^C
5710 GGGCCC
Asp700I 2462 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6372 GAACTTCTTC
BbrPI 1383 CACGTG CAC^GTG
1854 CACGTG
BglI 416 GCCTGTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2227 GCCAGCTTGGC
BsaAI 1383 CACGTG [CT]AC^GT[GA]
1854 CACGTG
BsaWI 2700 ACCGGA [AT]^CCGG[AT]
3507 ACCGGA
BsmBI 1957 CGTCTC CGTCTC(1/5)
2003 CGTCTC
BspLU11I 2916 ACATGT A^CATGT
3242 ACATGT
BsrDI 4003 CATTGC GCAATG(2/0)
4315 CATTGC
BsrFI 109 GCCGGT [GA]^CCGG[CT]
1400 GCCGGT
BstAPI 4258 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6322 GCAGACGATGC
BstEII 3215 GGTCACC G^GT[ACGT]ACC
4712 GGTCACC
BstPI 3215 GGTCACC G^GT[ACGT]ACC
4712 GGTCACC
Bsu36I 69 CCTGAGG CC^T[ACGT]AGG
1155 CCTGAGG
Cfr10I 109 GCCGGT [GA]^CCGG[CT]
1400 GCCGGT
DraI 3097 TTTAAA TTT^AAA
6497 TTTAAA
Eco81I 69 CCTGAGG CC^T[ACGT]AGG
1155 CCTGAGG
EcoO65I 3215 GGTCACC G^GT[ACGT]ACC
4712 GGTCACC
EcoRI 179 GAATTC G^AATTC
6401 GAATTC
HindIII 1497 AAGCTT A^AGCTT
5521 AAGCTT
HpaI 2580 GTTAAC GTT^AAC
5813 GTTAAC
NheI 2547 GCTAGC G^CTAGC
3144 GCTAGC
PmaCI 1383 CACGTG CAC^GTG
1854 CACGTG
PmlI 1383 CACGTG CAC^GTG
1854 CACGTG
ScaI 1026 AGTACT AGT^ACT
4680 AGTACT
TaqI 1770 TCGA T^CGA
1812 TCGA
TthHB8I 1770 TCGA T^CGA
1812 TCGA
XbaI 4820 TCTAGA T^CTAGA
5611 TCTAGA
XmnI 2462 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6372 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BfrI BglII BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XcmI XhoII