################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII BciVI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI KspI MamI MfeI MluI MroI MunI MvnI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SphI Sse8387I SspI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 1506 GACGTC GACGT^C Acc65I 5802 GGTACC G^GTACC Asp718I 5802 GGTACC G^GTACC AvrII 3046 CCTAGG C^CTAGG BamHI 3646 GGATCC G^GATCC BbsI 5411 GAAGAC GAAGAC(2/6) BcgI 4465 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 188 TGATCA T^GATCA BlnI 3046 CCTAGG C^CTAGG BlpI 4038 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4038 GCTCAGC GC^T[ACGT]AGC BpuAI 5411 GAAGAC GAAGAC(2/6) Bsp1407I 2056 TGTACA T^GTACA BsrGI 2056 TGTACA T^GTACA BssSI 264 CTCGTG CACGAG(-5/-1) BstXI 1780 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4038 GCTCAGC GC^T[ACGT]AGC DrdI 5364 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1943 GATATC GAT^ATC FbaI 188 TGATCA T^GATCA KpnI 5802 GGTACC GGTAC^C NdeI 1138 CATATG CA^TATG PshAI 1004 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfiI 2226 GGCCAGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1458 CCCGGG CCC^GGG SpeI 6047 ACTAGT A^CTAGT SspBI 2056 TGTACA T^GTACA SwaI 6496 ATTTAAAT ATTT^AAAT XmaCI 1458 CCCGGG C^CCGGG XmaI 1458 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 2217 GTCTAC GT^[AC][GT]AC 6033 GTCTAC AocI 69 CCTGAGG CC^T[ACGT]AGG 1155 CCTGAGG ApaI 3468 GGGCCC GGGCC^C 5710 GGGCCC Asp700I 2462 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6372 GAACTTCTTC BbrPI 1383 CACGTG CAC^GTG 1854 CACGTG BglI 416 GCCTGTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2227 GCCAGCTTGGC BsaAI 1383 CACGTG [CT]AC^GT[GA] 1854 CACGTG BsaWI 2700 ACCGGA [AT]^CCGG[AT] 3507 ACCGGA BsmBI 1957 CGTCTC CGTCTC(1/5) 2003 CGTCTC BspLU11I 2916 ACATGT A^CATGT 3242 ACATGT BsrDI 4003 CATTGC GCAATG(2/0) 4315 CATTGC BsrFI 109 GCCGGT [GA]^CCGG[CT] 1400 GCCGGT BstAPI 4258 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6322 GCAGACGATGC BstEII 3215 GGTCACC G^GT[ACGT]ACC 4712 GGTCACC BstPI 3215 GGTCACC G^GT[ACGT]ACC 4712 GGTCACC Bsu36I 69 CCTGAGG CC^T[ACGT]AGG 1155 CCTGAGG Cfr10I 109 GCCGGT [GA]^CCGG[CT] 1400 GCCGGT DraI 3097 TTTAAA TTT^AAA 6497 TTTAAA Eco81I 69 CCTGAGG CC^T[ACGT]AGG 1155 CCTGAGG EcoO65I 3215 GGTCACC G^GT[ACGT]ACC 4712 GGTCACC EcoRI 179 GAATTC G^AATTC 6401 GAATTC HindIII 1497 AAGCTT A^AGCTT 5521 AAGCTT HpaI 2580 GTTAAC GTT^AAC 5813 GTTAAC NheI 2547 GCTAGC G^CTAGC 3144 GCTAGC PmaCI 1383 CACGTG CAC^GTG 1854 CACGTG PmlI 1383 CACGTG CAC^GTG 1854 CACGTG ScaI 1026 AGTACT AGT^ACT 4680 AGTACT TaqI 1770 TCGA T^CGA 1812 TCGA TthHB8I 1770 TCGA T^CGA 1812 TCGA XbaI 4820 TCTAGA T^CTAGA 5611 TCTAGA XmnI 2462 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6372 GAACTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BfrI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoII