################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII BbeI BfrI BseAI BsiWI BsmI BspEI BspHI BssHII BstEII BstPI CpoI DraI DraIII DrdI Eam1105I EcoO65I FspI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI PacI PinAI PmeI PshBI Psp1406I PvuII RcaI RsrII SacII SalI SfoI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2368 GACGTC GACGT^C AccI 3751 GTATAC GT^[AC][GT]AC AccII 510 CGCG CG^CG Aor51HI 1477 AGCGCT AGC^GCT AvrII 4188 CCTAGG C^CTAGG BbrPI 2272 CACGTG CAC^GTG BciVI 3465 GTATCC GTATCC(6/5) BclI 4553 TGATCA T^GATCA BlnI 4188 CCTAGG C^CTAGG BsaWI 3890 TCCGGT [AT]^CCGG[AT] BspDI 225 ATCGAT AT^CGAT Bst1107I 3751 GTATAC GTA^TAC BstUI 510 CGCG CG^CG BstZ17I 3751 GTATAC GTA^TAC ClaI 225 ATCGAT AT^CGAT EagI 991 CGGCCG C^GGCCG EclXI 991 CGGCCG C^GGCCG Eco47III 1477 AGCGCT AGC^GCT Eco52I 991 CGGCCG C^GGCCG EcoRI 2857 GAATTC G^AATTC EcoRV 4261 GATATC GAT^ATC EcoT22I 3676 ATGCAT ATGCA^T FbaI 4553 TGATCA T^GATCA FseI 11 GGCCGGCC GGCCGG^CC HpaI 601 GTTAAC GTT^AAC MspA1I 1217 CAGCGG C[AC]G^C[GT]G MvnI 510 CGCG CG^CG NaeI 12 GCCGGC GCC^GGC NgoMIV 12 GCCGGC G^CCGGC NsiI 3676 ATGCAT ATGCA^T PaeR7I 1183 CTCGAG C^TCGAG PmaCI 2272 CACGTG CAC^GTG PmlI 2272 CACGTG CAC^GTG PshAI 1378 GACGACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 3884 ACCTGGT A^CC[AT]GGT SfiI 3289 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2504 GCATGC GCATG^C XhoI 1183 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AcyI 1129 GACGCC G[GA]^CG[CT]C 2368 GACGTC AlwNI 808 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 907 CAGAAGCTG ApaI 649 GGGCCC GGGCC^C 3556 GGGCCC Asp700I 2833 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4945 GAAAAGGTTC AspI 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACGACGTC BalI 964 TGGCCA TGG^CCA 3788 TGGCCA BamHI 1811 GGATCC G^GATCC 2358 GGATCC BglII 897 AGATCT A^GATCT 2062 AGATCT BlpI 2011 GCTGAGC GC^T[ACGT]AGC 4389 GCTAAGC Bpu1102I 2011 GCTGAGC GC^T[ACGT]AGC 4389 GCTAAGC BsaAI 2272 CACGTG [CT]AC^GT[GA] 2914 TACGTG BsaHI 1129 GACGCC G[GA]^CG[CT]C 2368 GACGTC BspLU11I 2717 ACATGT A^CATGT 4821 ACATGT BsrBI 1059 GAGCGG CCGCTC(-3/-3) 2934 GAGCGG BsrFI 12 GCCGGC [GA]^CCGG[CT] 637 GCCGGT BstAPI 807 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1770 GCAGAAGATGC BstBI 3512 TTCGAA TT^CGAA 4897 TTCGAA BstXI 1166 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2126 CCAAGGCCCTGG CelII 2011 GCTGAGC GC^T[ACGT]AGC 4389 GCTAAGC Cfr10I 12 GCCGGC [GA]^CCGG[CT] 637 GCCGGT EcoNI 2292 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3295 CCTGGCCCAGG Hin1I 1129 GACGCC G[GA]^CG[CT]C 2368 GACGTC MluNI 964 TGGCCA TGG^CCA 3788 TGGCCA MscI 964 TGGCCA TGG^CCA 3788 TGGCCA NcoI 1505 CCATGG C^CATGG 3231 CCATGG NspV 3512 TTCGAA TT^CGAA 4897 TTCGAA PflFI 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACGACGTC PvuI 22 CGATCG CGAT^CG 469 CGATCG SapI 3445 GAAGAGC GCTCTTC(1/4) 2097 GCTCTTC SbfI 1995 CCTGCAGG CCTGCA^GG 3984 CCTGCAGG ScaI 2777 AGTACT AGT^ACT 3941 AGTACT SfuI 3512 TTCGAA TT^CGAA 4897 TTCGAA SmaI 2254 CCCGGG CCC^GGG 4833 CCCGGG Sse8387I 1995 CCTGCAGG CCTGCA^GG 3984 CCTGCAGG SspI 3858 AATATT AAT^ATT 4570 AATATT Tth111I 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACGACGTC XmaCI 2254 CCCGGG C^CCGGG 4833 CCCGGG XmaI 2254 CCCGGG C^CCGGG 4833 CCCGGG XmnI 2833 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4945 GAAAAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII