##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BfrI BseAI BsiWI BsmI BspEI
BspHI BssHII BstEII BstPI CpoI
DraI DraIII DrdI Eam1105I EcoO65I
FspI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI PacI PinAI
PmeI PshBI Psp1406I PvuII RcaI
RsrII SacII SalI SfoI SgrAI
SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2368 GACGTC GACGT^C
AccI 3751 GTATAC GT^[AC][GT]AC
AccII 510 CGCG CG^CG
Aor51HI 1477 AGCGCT AGC^GCT
AvrII 4188 CCTAGG C^CTAGG
BbrPI 2272 CACGTG CAC^GTG
BciVI 3465 GTATCC GTATCC(6/5)
BclI 4553 TGATCA T^GATCA
BlnI 4188 CCTAGG C^CTAGG
BsaWI 3890 TCCGGT [AT]^CCGG[AT]
BspDI 225 ATCGAT AT^CGAT
Bst1107I 3751 GTATAC GTA^TAC
BstUI 510 CGCG CG^CG
BstZ17I 3751 GTATAC GTA^TAC
ClaI 225 ATCGAT AT^CGAT
EagI 991 CGGCCG C^GGCCG
EclXI 991 CGGCCG C^GGCCG
Eco47III 1477 AGCGCT AGC^GCT
Eco52I 991 CGGCCG C^GGCCG
EcoRI 2857 GAATTC G^AATTC
EcoRV 4261 GATATC GAT^ATC
EcoT22I 3676 ATGCAT ATGCA^T
FbaI 4553 TGATCA T^GATCA
FseI 11 GGCCGGCC GGCCGG^CC
HpaI 601 GTTAAC GTT^AAC
MspA1I 1217 CAGCGG C[AC]G^C[GT]G
MvnI 510 CGCG CG^CG
NaeI 12 GCCGGC GCC^GGC
NgoMIV 12 GCCGGC G^CCGGC
NsiI 3676 ATGCAT ATGCA^T
PaeR7I 1183 CTCGAG C^TCGAG
PmaCI 2272 CACGTG CAC^GTG
PmlI 2272 CACGTG CAC^GTG
PshAI 1378 GACGACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3884 ACCTGGT A^CC[AT]GGT
SfiI 3289 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2504 GCATGC GCATG^C
XhoI 1183 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 1129 GACGCC G[GA]^CG[CT]C
2368 GACGTC
AlwNI 808 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
907 CAGAAGCTG
ApaI 649 GGGCCC GGGCC^C
3556 GGGCCC
Asp700I 2833 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4945 GAAAAGGTTC
AspI 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
BalI 964 TGGCCA TGG^CCA
3788 TGGCCA
BamHI 1811 GGATCC G^GATCC
2358 GGATCC
BglII 897 AGATCT A^GATCT
2062 AGATCT
BlpI 2011 GCTGAGC GC^T[ACGT]AGC
4389 GCTAAGC
Bpu1102I 2011 GCTGAGC GC^T[ACGT]AGC
4389 GCTAAGC
BsaAI 2272 CACGTG [CT]AC^GT[GA]
2914 TACGTG
BsaHI 1129 GACGCC G[GA]^CG[CT]C
2368 GACGTC
BspLU11I 2717 ACATGT A^CATGT
4821 ACATGT
BsrBI 1059 GAGCGG CCGCTC(-3/-3)
2934 GAGCGG
BsrFI 12 GCCGGC [GA]^CCGG[CT]
637 GCCGGT
BstAPI 807 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1770 GCAGAAGATGC
BstBI 3512 TTCGAA TT^CGAA
4897 TTCGAA
BstXI 1166 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2126 CCAAGGCCCTGG
CelII 2011 GCTGAGC GC^T[ACGT]AGC
4389 GCTAAGC
Cfr10I 12 GCCGGC [GA]^CCGG[CT]
637 GCCGGT
EcoNI 2292 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3295 CCTGGCCCAGG
Hin1I 1129 GACGCC G[GA]^CG[CT]C
2368 GACGTC
MluNI 964 TGGCCA TGG^CCA
3788 TGGCCA
MscI 964 TGGCCA TGG^CCA
3788 TGGCCA
NcoI 1505 CCATGG C^CATGG
3231 CCATGG
NspV 3512 TTCGAA TT^CGAA
4897 TTCGAA
PflFI 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
PvuI 22 CGATCG CGAT^CG
469 CGATCG
SapI 3445 GAAGAGC GCTCTTC(1/4)
2097 GCTCTTC
SbfI 1995 CCTGCAGG CCTGCA^GG
3984 CCTGCAGG
ScaI 2777 AGTACT AGT^ACT
3941 AGTACT
SfuI 3512 TTCGAA TT^CGAA
4897 TTCGAA
SmaI 2254 CCCGGG CCC^GGG
4833 CCCGGG
Sse8387I 1995 CCTGCAGG CCTGCA^GG
3984 CCTGCAGG
SspI 3858 AATATT AAT^ATT
4570 AATATT
Tth111I 1018 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACGACGTC
XmaCI 2254 CCCGGG C^CCGGG
4833 CCCGGG
XmaI 2254 CCCGGG C^CCGGG
4833 CCCGGG
XmnI 2833 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4945 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII