##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AspI        BamHI       
BbeI        BciVI       BsaBI       BseAI       BsiWI       
Bsp1407I    BspEI       BsrFI       BsrGI       BssHII      
BstBI       BstEII      BstPI       Cfr10I      CpoI        
DraIII      Eco47III    EcoO65I     EcoRV       FseI        
KasI        MamI        MluI        MroI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       Psp1406I    RsrII       SalI        
SapI        SbfI        SfoI        SfuI        SgrAI       
SpeI        Sse8387I    SspBI       SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        560  GGTACC               G^GTACC
AccI          517  GTATAC               GT^[AC][GT]AC
AcyI          677  GGCGTC               G[GA]^CG[CT]C
AocI         4505  CCTCAGG              CC^T[ACGT]AGG
ApaI         3570  GGGCCC               GGGCC^C
Asp700I      2344  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       560  GGTACC               G^GTACC
AvrII        3930  CCTAGG               C^CTAGG
BbrPI        3800  CACGTG               CAC^GTG
BcgI         5702  GCATGGAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          941  TGATCA               T^GATCA
BlnI         3930  CCTAGG               C^CTAGG
BsaHI         677  GGCGTC               G[GA]^CG[CT]C
BspDI        3197  ATCGAT               AT^CGAT
BssSI        4956  CACGAG               CACGAG(-5/-1)
Bst1107I      517  GTATAC               GTA^TAC
BstAPI        453  GCAAGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       517  GTATAC               GTA^TAC
Bsu36I       4505  CCTCAGG              CC^T[ACGT]AGG
ClaI         3197  ATCGAT               AT^CGAT
DrdI          363  GACAGTGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          239  CGGCCG               C^GGCCG
EclXI         239  CGGCCG               C^GGCCG
Eco52I        239  CGGCCG               C^GGCCG
Eco81I       4505  CCTCAGG              CC^T[ACGT]AGG
FbaI          941  TGATCA               T^GATCA
HgaI          678  GCGTC                GACGC(5/10)
Hin1I         677  GGCGTC               G[GA]^CG[CT]C
HincII       4578  GTTAAC               GT[CT]^[GA]AC
HindII       4578  GTTAAC               GT[CT]^[GA]AC
HindIII      5047  AAGCTT               A^AGCTT
HpaI         4578  GTTAAC               GTT^AAC
KpnI          560  GGTACC               GGTAC^C
KspI           94  CCGCGG               CCGC^GG
MfeI          664  CAATTG               C^AATTG
MunI          664  CAATTG               C^AATTG
PaeR7I       1278  CTCGAG               C^TCGAG
PmaCI        3800  CACGTG               CAC^GTG
PmlI         3800  CACGTG               CAC^GTG
PvuI         3199  CGATCG               CGAT^CG
SacII          94  CCGCGG               CCGC^GG
ScaI          856  AGTACT               AGT^ACT
SexAI        1364  ACCAGGT              A^CC[AT]GGT
SfiI          240  GGCCGACCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1336  GCATGC               GCATG^C
XcmI         3749  CCACATAAAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1278  CTCGAG               C^TCGAG
XmnI         2344  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflII        4415  CTTAAG               C^TTAAG
             5376  CTTAAG              
AhdI         3154  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3204  GACGTTGAGTC         
AseI         2533  ATTAAT               AT^TAAT
             6023  ATTAAT              
AsnI         2533  ATTAAT               AT^TAAT
             6023  ATTAAT              
AspEI        3154  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3204  GACGTTGAGTC         
AviII         104  TGCGCA               TGC^GCA
              257  TGCGCA              
BalI          640  TGGCCA               TGG^CCA
             2382  TGGCCA              
BfrI         4415  CTTAAG               C^TTAAG
             5376  CTTAAG              
BglI          241  GCCGACCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3962  GCCAGGCTGGC         
BglII        2137  AGATCT               A^GATCT
             4993  AGATCT              
BlpI         5143  GCTCAGC              GC^T[ACGT]AGC
             5813  GCTGAGC             
Bpu1102I     5143  GCTCAGC              GC^T[ACGT]AGC
             5813  GCTGAGC             
BsmBI        2443  CGTCTC               CGTCTC(1/5)
             2557  CGTCTC              
BspLU11I      486  ACATGT               A^CATGT
             1293  ACATGT              
BsrBI        5077  CCGCTC               CCGCTC(-3/-3)
             2210  GAGCGG              
CelII        5143  GCTCAGC              GC^T[ACGT]AGC
             5813  GCTGAGC             
Eam1105I     3154  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3204  GACGTTGAGTC         
EcoRI         751  GAATTC               G^AATTC
             2882  GAATTC              
FspI          104  TGCGCA               TGC^GCA
              257  TGCGCA              
HaeII         217  AGCGCC               [GA]GCGC^[CT]
             5572  AGCGCC              
MluNI         640  TGGCCA               TGG^CCA
             2382  TGGCCA              
MscI          640  TGGCCA               TGG^CCA
             2382  TGGCCA              
NcoI         3188  CCATGG               C^CATGG
             5777  CCATGG              
PflMI        3183  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3574  CCAAGAAGTGG         
PshBI        2533  ATTAAT               AT^TAAT
             6023  ATTAAT              
SacI         1531  GAGCTC               GAGCT^C
             5192  GAGCTC              
SmaI          229  CCCGGG               CCC^GGG
             2663  CCCGGG              
SnaBI         959  TACGTA               TAC^GTA
             1104  TACGTA              
StuI         5135  AGGCCT               AGG^CCT
             5181  AGGCCT              
Van91I       3183  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3574  CCAAGAAGTGG         
XbaI          699  TCTAGA               T^CTAGA
             5976  TCTAGA              
XmaCI         229  CCCGGG               C^CCGGG
             2663  CCCGGG              
XmaI          229  CCCGGG               C^CCGGG
             2663  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII