##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AspI BamHI
BbeI BciVI BsaBI BseAI BsiWI
Bsp1407I BspEI BsrFI BsrGI BssHII
BstBI BstEII BstPI Cfr10I CpoI
DraIII Eco47III EcoO65I EcoRV FseI
KasI MamI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI Psp1406I RsrII SalI
SapI SbfI SfoI SfuI SgrAI
SpeI Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 560 GGTACC G^GTACC
AccI 517 GTATAC GT^[AC][GT]AC
AcyI 677 GGCGTC G[GA]^CG[CT]C
AocI 4505 CCTCAGG CC^T[ACGT]AGG
ApaI 3570 GGGCCC GGGCC^C
Asp700I 2344 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 560 GGTACC G^GTACC
AvrII 3930 CCTAGG C^CTAGG
BbrPI 3800 CACGTG CAC^GTG
BcgI 5702 GCATGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 941 TGATCA T^GATCA
BlnI 3930 CCTAGG C^CTAGG
BsaHI 677 GGCGTC G[GA]^CG[CT]C
BspDI 3197 ATCGAT AT^CGAT
BssSI 4956 CACGAG CACGAG(-5/-1)
Bst1107I 517 GTATAC GTA^TAC
BstAPI 453 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 517 GTATAC GTA^TAC
Bsu36I 4505 CCTCAGG CC^T[ACGT]AGG
ClaI 3197 ATCGAT AT^CGAT
DrdI 363 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 239 CGGCCG C^GGCCG
EclXI 239 CGGCCG C^GGCCG
Eco52I 239 CGGCCG C^GGCCG
Eco81I 4505 CCTCAGG CC^T[ACGT]AGG
FbaI 941 TGATCA T^GATCA
HgaI 678 GCGTC GACGC(5/10)
Hin1I 677 GGCGTC G[GA]^CG[CT]C
HincII 4578 GTTAAC GT[CT]^[GA]AC
HindII 4578 GTTAAC GT[CT]^[GA]AC
HindIII 5047 AAGCTT A^AGCTT
HpaI 4578 GTTAAC GTT^AAC
KpnI 560 GGTACC GGTAC^C
KspI 94 CCGCGG CCGC^GG
MfeI 664 CAATTG C^AATTG
MunI 664 CAATTG C^AATTG
PaeR7I 1278 CTCGAG C^TCGAG
PmaCI 3800 CACGTG CAC^GTG
PmlI 3800 CACGTG CAC^GTG
PvuI 3199 CGATCG CGAT^CG
SacII 94 CCGCGG CCGC^GG
ScaI 856 AGTACT AGT^ACT
SexAI 1364 ACCAGGT A^CC[AT]GGT
SfiI 240 GGCCGACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1336 GCATGC GCATG^C
XcmI 3749 CCACATAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1278 CTCGAG C^TCGAG
XmnI 2344 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 4415 CTTAAG C^TTAAG
5376 CTTAAG
AhdI 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3204 GACGTTGAGTC
AseI 2533 ATTAAT AT^TAAT
6023 ATTAAT
AsnI 2533 ATTAAT AT^TAAT
6023 ATTAAT
AspEI 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3204 GACGTTGAGTC
AviII 104 TGCGCA TGC^GCA
257 TGCGCA
BalI 640 TGGCCA TGG^CCA
2382 TGGCCA
BfrI 4415 CTTAAG C^TTAAG
5376 CTTAAG
BglI 241 GCCGACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3962 GCCAGGCTGGC
BglII 2137 AGATCT A^GATCT
4993 AGATCT
BlpI 5143 GCTCAGC GC^T[ACGT]AGC
5813 GCTGAGC
Bpu1102I 5143 GCTCAGC GC^T[ACGT]AGC
5813 GCTGAGC
BsmBI 2443 CGTCTC CGTCTC(1/5)
2557 CGTCTC
BspLU11I 486 ACATGT A^CATGT
1293 ACATGT
BsrBI 5077 CCGCTC CCGCTC(-3/-3)
2210 GAGCGG
CelII 5143 GCTCAGC GC^T[ACGT]AGC
5813 GCTGAGC
Eam1105I 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3204 GACGTTGAGTC
EcoRI 751 GAATTC G^AATTC
2882 GAATTC
FspI 104 TGCGCA TGC^GCA
257 TGCGCA
HaeII 217 AGCGCC [GA]GCGC^[CT]
5572 AGCGCC
MluNI 640 TGGCCA TGG^CCA
2382 TGGCCA
MscI 640 TGGCCA TGG^CCA
2382 TGGCCA
NcoI 3188 CCATGG C^CATGG
5777 CCATGG
PflMI 3183 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3574 CCAAGAAGTGG
PshBI 2533 ATTAAT AT^TAAT
6023 ATTAAT
SacI 1531 GAGCTC GAGCT^C
5192 GAGCTC
SmaI 229 CCCGGG CCC^GGG
2663 CCCGGG
SnaBI 959 TACGTA TAC^GTA
1104 TACGTA
StuI 5135 AGGCCT AGG^CCT
5181 AGGCCT
Van91I 3183 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3574 CCAAGAAGTGG
XbaI 699 TCTAGA T^CTAGA
5976 TCTAGA
XmaCI 229 CCCGGG C^CCGGG
2663 CCCGGG
XmaI 229 CCCGGG C^CCGGG
2663 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII