################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AspI BamHI BbeI BciVI BsaBI BseAI BsiWI Bsp1407I BspEI BsrFI BsrGI BssHII BstBI BstEII BstPI Cfr10I CpoI DraIII Eco47III EcoO65I EcoRV FseI KasI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI Psp1406I RsrII SalI SapI SbfI SfoI SfuI SgrAI SpeI Sse8387I SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 560 GGTACC G^GTACC AccI 517 GTATAC GT^[AC][GT]AC AcyI 677 GGCGTC G[GA]^CG[CT]C AocI 4505 CCTCAGG CC^T[ACGT]AGG ApaI 3570 GGGCCC GGGCC^C Asp700I 2344 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 560 GGTACC G^GTACC AvrII 3930 CCTAGG C^CTAGG BbrPI 3800 CACGTG CAC^GTG BcgI 5702 GCATGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 941 TGATCA T^GATCA BlnI 3930 CCTAGG C^CTAGG BsaHI 677 GGCGTC G[GA]^CG[CT]C BspDI 3197 ATCGAT AT^CGAT BssSI 4956 CACGAG CACGAG(-5/-1) Bst1107I 517 GTATAC GTA^TAC BstAPI 453 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 517 GTATAC GTA^TAC Bsu36I 4505 CCTCAGG CC^T[ACGT]AGG ClaI 3197 ATCGAT AT^CGAT DrdI 363 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 239 CGGCCG C^GGCCG EclXI 239 CGGCCG C^GGCCG Eco52I 239 CGGCCG C^GGCCG Eco81I 4505 CCTCAGG CC^T[ACGT]AGG FbaI 941 TGATCA T^GATCA HgaI 678 GCGTC GACGC(5/10) Hin1I 677 GGCGTC G[GA]^CG[CT]C HincII 4578 GTTAAC GT[CT]^[GA]AC HindII 4578 GTTAAC GT[CT]^[GA]AC HindIII 5047 AAGCTT A^AGCTT HpaI 4578 GTTAAC GTT^AAC KpnI 560 GGTACC GGTAC^C KspI 94 CCGCGG CCGC^GG MfeI 664 CAATTG C^AATTG MunI 664 CAATTG C^AATTG PaeR7I 1278 CTCGAG C^TCGAG PmaCI 3800 CACGTG CAC^GTG PmlI 3800 CACGTG CAC^GTG PvuI 3199 CGATCG CGAT^CG SacII 94 CCGCGG CCGC^GG ScaI 856 AGTACT AGT^ACT SexAI 1364 ACCAGGT A^CC[AT]GGT SfiI 240 GGCCGACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1336 GCATGC GCATG^C XcmI 3749 CCACATAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1278 CTCGAG C^TCGAG XmnI 2344 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflII 4415 CTTAAG C^TTAAG 5376 CTTAAG AhdI 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3204 GACGTTGAGTC AseI 2533 ATTAAT AT^TAAT 6023 ATTAAT AsnI 2533 ATTAAT AT^TAAT 6023 ATTAAT AspEI 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3204 GACGTTGAGTC AviII 104 TGCGCA TGC^GCA 257 TGCGCA BalI 640 TGGCCA TGG^CCA 2382 TGGCCA BfrI 4415 CTTAAG C^TTAAG 5376 CTTAAG BglI 241 GCCGACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3962 GCCAGGCTGGC BglII 2137 AGATCT A^GATCT 4993 AGATCT BlpI 5143 GCTCAGC GC^T[ACGT]AGC 5813 GCTGAGC Bpu1102I 5143 GCTCAGC GC^T[ACGT]AGC 5813 GCTGAGC BsmBI 2443 CGTCTC CGTCTC(1/5) 2557 CGTCTC BspLU11I 486 ACATGT A^CATGT 1293 ACATGT BsrBI 5077 CCGCTC CCGCTC(-3/-3) 2210 GAGCGG CelII 5143 GCTCAGC GC^T[ACGT]AGC 5813 GCTGAGC Eam1105I 3154 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3204 GACGTTGAGTC EcoRI 751 GAATTC G^AATTC 2882 GAATTC FspI 104 TGCGCA TGC^GCA 257 TGCGCA HaeII 217 AGCGCC [GA]GCGC^[CT] 5572 AGCGCC MluNI 640 TGGCCA TGG^CCA 2382 TGGCCA MscI 640 TGGCCA TGG^CCA 2382 TGGCCA NcoI 3188 CCATGG C^CATGG 5777 CCATGG PflMI 3183 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3574 CCAAGAAGTGG PshBI 2533 ATTAAT AT^TAAT 6023 ATTAAT SacI 1531 GAGCTC GAGCT^C 5192 GAGCTC SmaI 229 CCCGGG CCC^GGG 2663 CCCGGG SnaBI 959 TACGTA TAC^GTA 1104 TACGTA StuI 5135 AGGCCT AGG^CCT 5181 AGGCCT Van91I 3183 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3574 CCAAGAAGTGG XbaI 699 TCTAGA T^CTAGA 5976 TCTAGA XmaCI 229 CCCGGG C^CCGGG 2663 CCCGGG XmaI 229 CCCGGG C^CCGGG 2663 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII