################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AhdI AocI Aor51HI AscI Asp718I AspEI AvrII BalI BbeI BbrPI BcgI BciVI BlnI BsaAI BsiEI BsiWI BsmBI BsrBI BssHII BstBI Bsu36I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I FseI HindIII KasI KpnI MluI MluNI MscI NaeI NarI NgoMIV NotI NspV PaeR7I PmaCI PmeI PmlI PshAI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 2479 TCCGGA T^CCGGA AclI 7252 AACGTT AA^CGTT AcyI 3098 GGCGTC G[GA]^CG[CT]C AflII 7419 CTTAAG C^TTAAG AgeI 1802 ACCGGT A^CCGGT Alw44I 5798 GTGCAC G^TGCAC ApaI 3297 GGGCCC GGGCC^C ApaLI 5798 GTGCAC G^TGCAC AviII 4209 TGCGCA TGC^GCA BamHI 5005 GGATCC G^GATCC BclI 4084 TGATCA T^GATCA BfrI 7419 CTTAAG C^TTAAG BglI 640 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2412 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2412 GCTCAGC GC^T[ACGT]AGC BsaHI 3098 GGCGTC G[GA]^CG[CT]C BseAI 2479 TCCGGA T^CCGGA BspDI 3116 ATCGAT AT^CGAT BspEI 2479 TCCGGA T^CCGGA BspHI 1026 TCATGA T^CATGA BspMI 3697 GCAGGT ACCTGC(4/8) BsrFI 1802 ACCGGT [GA]^CCGG[CT] Bst1107I 5561 GTATAC GTA^TAC BstXI 2426 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 5561 GTATAC GTA^TAC CelII 2412 GCTCAGC GC^T[ACGT]AGC Cfr10I 1802 ACCGGT [GA]^CCGG[CT] ClaI 3116 ATCGAT AT^CGAT DrdI 3636 GACTCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2608 TGGCCG [CT]^GGCC[GA] FbaI 4084 TGATCA T^GATCA FspI 4209 TGCGCA TGC^GCA Hin1I 3098 GGCGTC G[GA]^CG[CT]C KspI 9 CCGCGG CCGC^GG MfeI 1125 CAATTG C^AATTG MroI 2479 TCCGGA T^CCGGA MunI 1125 CAATTG C^AATTG NcoI 2207 CCATGG C^CATGG NheI 4975 GCTAGC G^CTAGC NruI 520 TCGCGA TCG^CGA PacI 6101 TTAATTAA TTAAT^TAA PinAI 1802 ACCGGT A^CCGGT Psp1406I 7252 AACGTT AA^CGTT RcaI 1026 TCATGA T^CATGA SacII 9 CCGCGG CCGC^GG SapI 110 GAAGAGC GCTCTTC(1/4) SmaI 473 CCCGGG CCC^GGG StuI 3501 AGGCCT AGG^CCT XbaI 4409 TCTAGA T^CTAGA XmaCI 473 CCCGGG C^CCGGG XmaI 473 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3885 GACACAGTC BanI 27 GGCACC G^G[CT][GA]CC 6476 GGCACC BbsI 922 GAAGAC GAAGAC(2/6) 2569 GTCTTC BbvCI 3543 CCTCAGC CCTCAGC(-5/-2) 916 GCTGAGG BpuAI 922 GAAGAC GAAGAC(2/6) 2569 GTCTTC BsmI 6644 GAATGC GAATGC(1/-1) 2834 GCATTC Bsp1407I 5473 TGTACA T^GTACA 5536 TGTACA BsrGI 5473 TGTACA T^GTACA 5536 TGTACA BstAPI 610 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3562 GCATGTCCTGC EcoNI 3418 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6174 CCTGCCACAGG EcoRV 4426 GATATC GAT^ATC 5615 GATATC EcoT14I 630 CCAAGG C^C[AT][AT]GG 2207 CCATGG HaeII 1054 AGCGCC [GA]GCGC^[CT] 1946 GGCGCT HgaI 3099 GCGTC GACGC(5/10) 7266 GCGTC HpaI 3986 GTTAAC GTT^AAC 5419 GTTAAC NdeI 928 CATATG CA^TATG 3258 CATATG PflFI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3885 GACACAGTC PflMI 2258 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5869 CCAGCTACTGG SphI 3693 GCATGC GCATG^C 4759 GCATGC SspBI 5473 TGTACA T^GTACA 5536 TGTACA StyI 630 CCAAGG C^C[AT][AT]GG 2207 CCATGG Tth111I 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3885 GACACAGTC Van91I 2258 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5869 CCAGCTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BglII BmyI BpmI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI