##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AhdI        AocI        Aor51HI     
AscI        Asp718I     AspEI       AvrII       BalI        
BbeI        BbrPI       BcgI        BciVI       BlnI        
BsaAI       BsiEI       BsiWI       BsmBI       BsrBI       
BssHII      BstBI       Bsu36I      CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      FseI        HindIII     KasI        KpnI        
MluI        MluNI       MscI        NaeI        NarI        
NgoMIV      NotI        NspV        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       PvuI        RsrII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       2479  TCCGGA               T^CCGGA
AclI         7252  AACGTT               AA^CGTT
AcyI         3098  GGCGTC               G[GA]^CG[CT]C
AflII        7419  CTTAAG               C^TTAAG
AgeI         1802  ACCGGT               A^CCGGT
Alw44I       5798  GTGCAC               G^TGCAC
ApaI         3297  GGGCCC               GGGCC^C
ApaLI        5798  GTGCAC               G^TGCAC
AviII        4209  TGCGCA               TGC^GCA
BamHI        5005  GGATCC               G^GATCC
BclI         4084  TGATCA               T^GATCA
BfrI         7419  CTTAAG               C^TTAAG
BglI          640  GCCCCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2412  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2412  GCTCAGC              GC^T[ACGT]AGC
BsaHI        3098  GGCGTC               G[GA]^CG[CT]C
BseAI        2479  TCCGGA               T^CCGGA
BspDI        3116  ATCGAT               AT^CGAT
BspEI        2479  TCCGGA               T^CCGGA
BspHI        1026  TCATGA               T^CATGA
BspMI        3697  GCAGGT               ACCTGC(4/8)
BsrFI        1802  ACCGGT               [GA]^CCGG[CT]
Bst1107I     5561  GTATAC               GTA^TAC
BstXI        2426  CCAAAATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      5561  GTATAC               GTA^TAC
CelII        2412  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1802  ACCGGT               [GA]^CCGG[CT]
ClaI         3116  ATCGAT               AT^CGAT
DrdI         3636  GACTCTCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         2608  TGGCCG               [CT]^GGCC[GA]
FbaI         4084  TGATCA               T^GATCA
FspI         4209  TGCGCA               TGC^GCA
Hin1I        3098  GGCGTC               G[GA]^CG[CT]C
KspI            9  CCGCGG               CCGC^GG
MfeI         1125  CAATTG               C^AATTG
MroI         2479  TCCGGA               T^CCGGA
MunI         1125  CAATTG               C^AATTG
NcoI         2207  CCATGG               C^CATGG
NheI         4975  GCTAGC               G^CTAGC
NruI          520  TCGCGA               TCG^CGA
PacI         6101  TTAATTAA             TTAAT^TAA
PinAI        1802  ACCGGT               A^CCGGT
Psp1406I     7252  AACGTT               AA^CGTT
RcaI         1026  TCATGA               T^CATGA
SacII           9  CCGCGG               CCGC^GG
SapI          110  GAAGAGC              GCTCTTC(1/4)
SmaI          473  CCCGGG               CCC^GGG
StuI         3501  AGGCCT               AGG^CCT
XbaI         4409  TCTAGA               T^CTAGA
XmaCI         473  CCCGGG               C^CCGGG
XmaI          473  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspI          119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3885  GACACAGTC           
BanI           27  GGCACC               G^G[CT][GA]CC
             6476  GGCACC              
BbsI          922  GAAGAC               GAAGAC(2/6)
             2569  GTCTTC              
BbvCI        3543  CCTCAGC              CCTCAGC(-5/-2)
              916  GCTGAGG             
BpuAI         922  GAAGAC               GAAGAC(2/6)
             2569  GTCTTC              
BsmI         6644  GAATGC               GAATGC(1/-1)
             2834  GCATTC              
Bsp1407I     5473  TGTACA               T^GTACA
             5536  TGTACA              
BsrGI        5473  TGTACA               T^GTACA
             5536  TGTACA              
BstAPI        610  GCAAGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3562  GCATGTCCTGC         
EcoNI        3418  CCTGGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             6174  CCTGCCACAGG         
EcoRV        4426  GATATC               GAT^ATC
             5615  GATATC              
EcoT14I       630  CCAAGG               C^C[AT][AT]GG
             2207  CCATGG              
HaeII        1054  AGCGCC               [GA]GCGC^[CT]
             1946  GGCGCT              
HgaI         3099  GCGTC                GACGC(5/10)
             7266  GCGTC               
HpaI         3986  GTTAAC               GTT^AAC
             5419  GTTAAC              
NdeI          928  CATATG               CA^TATG
             3258  CATATG              
PflFI         119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3885  GACACAGTC           
PflMI        2258  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5869  CCAGCTACTGG         
SphI         3693  GCATGC               GCATG^C
             4759  GCATGC              
SspBI        5473  TGTACA               T^GTACA
             5536  TGTACA              
StyI          630  CCAAGG               C^C[AT][AT]GG
             2207  CCATGG              
Tth111I       119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3885  GACACAGTC           
Van91I       2258  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5869  CCAGCTACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        Asp700I     AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BsaBI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspLU11I    BsrDI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EarI        EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoT22I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PshBI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI