##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AhdI AocI Aor51HI
AscI Asp718I AspEI AvrII BalI
BbeI BbrPI BcgI BciVI BlnI
BsaAI BsiEI BsiWI BsmBI BsrBI
BssHII BstBI Bsu36I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I FseI HindIII KasI KpnI
MluI MluNI MscI NaeI NarI
NgoMIV NotI NspV PaeR7I PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 2479 TCCGGA T^CCGGA
AclI 7252 AACGTT AA^CGTT
AcyI 3098 GGCGTC G[GA]^CG[CT]C
AflII 7419 CTTAAG C^TTAAG
AgeI 1802 ACCGGT A^CCGGT
Alw44I 5798 GTGCAC G^TGCAC
ApaI 3297 GGGCCC GGGCC^C
ApaLI 5798 GTGCAC G^TGCAC
AviII 4209 TGCGCA TGC^GCA
BamHI 5005 GGATCC G^GATCC
BclI 4084 TGATCA T^GATCA
BfrI 7419 CTTAAG C^TTAAG
BglI 640 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2412 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2412 GCTCAGC GC^T[ACGT]AGC
BsaHI 3098 GGCGTC G[GA]^CG[CT]C
BseAI 2479 TCCGGA T^CCGGA
BspDI 3116 ATCGAT AT^CGAT
BspEI 2479 TCCGGA T^CCGGA
BspHI 1026 TCATGA T^CATGA
BspMI 3697 GCAGGT ACCTGC(4/8)
BsrFI 1802 ACCGGT [GA]^CCGG[CT]
Bst1107I 5561 GTATAC GTA^TAC
BstXI 2426 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5561 GTATAC GTA^TAC
CelII 2412 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1802 ACCGGT [GA]^CCGG[CT]
ClaI 3116 ATCGAT AT^CGAT
DrdI 3636 GACTCTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2608 TGGCCG [CT]^GGCC[GA]
FbaI 4084 TGATCA T^GATCA
FspI 4209 TGCGCA TGC^GCA
Hin1I 3098 GGCGTC G[GA]^CG[CT]C
KspI 9 CCGCGG CCGC^GG
MfeI 1125 CAATTG C^AATTG
MroI 2479 TCCGGA T^CCGGA
MunI 1125 CAATTG C^AATTG
NcoI 2207 CCATGG C^CATGG
NheI 4975 GCTAGC G^CTAGC
NruI 520 TCGCGA TCG^CGA
PacI 6101 TTAATTAA TTAAT^TAA
PinAI 1802 ACCGGT A^CCGGT
Psp1406I 7252 AACGTT AA^CGTT
RcaI 1026 TCATGA T^CATGA
SacII 9 CCGCGG CCGC^GG
SapI 110 GAAGAGC GCTCTTC(1/4)
SmaI 473 CCCGGG CCC^GGG
StuI 3501 AGGCCT AGG^CCT
XbaI 4409 TCTAGA T^CTAGA
XmaCI 473 CCCGGG C^CCGGG
XmaI 473 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3885 GACACAGTC
BanI 27 GGCACC G^G[CT][GA]CC
6476 GGCACC
BbsI 922 GAAGAC GAAGAC(2/6)
2569 GTCTTC
BbvCI 3543 CCTCAGC CCTCAGC(-5/-2)
916 GCTGAGG
BpuAI 922 GAAGAC GAAGAC(2/6)
2569 GTCTTC
BsmI 6644 GAATGC GAATGC(1/-1)
2834 GCATTC
Bsp1407I 5473 TGTACA T^GTACA
5536 TGTACA
BsrGI 5473 TGTACA T^GTACA
5536 TGTACA
BstAPI 610 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3562 GCATGTCCTGC
EcoNI 3418 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6174 CCTGCCACAGG
EcoRV 4426 GATATC GAT^ATC
5615 GATATC
EcoT14I 630 CCAAGG C^C[AT][AT]GG
2207 CCATGG
HaeII 1054 AGCGCC [GA]GCGC^[CT]
1946 GGCGCT
HgaI 3099 GCGTC GACGC(5/10)
7266 GCGTC
HpaI 3986 GTTAAC GTT^AAC
5419 GTTAAC
NdeI 928 CATATG CA^TATG
3258 CATATG
PflFI 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3885 GACACAGTC
PflMI 2258 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5869 CCAGCTACTGG
SphI 3693 GCATGC GCATG^C
4759 GCATGC
SspBI 5473 TGTACA T^GTACA
5536 TGTACA
StyI 630 CCAAGG C^C[AT][AT]GG
2207 CCATGG
Tth111I 119 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3885 GACACAGTC
Van91I 2258 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5869 CCAGCTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoO65I EcoRI EcoRII
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI