################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AatII AcsI AflII ApoI AscI AseI AsnI BfrI BglII BsaBI BsiWI BsmBI BssSI Bst1107I BstBI BstZ17I CpoI EcoRI EcoRV FseI HincII HindII HpaI MamI MfeI MluI MunI NotI NruI NspV PacI PmeI PshAI PshBI PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 3930 GTCTAC GT^[AC][GT]AC AclI 4748 AACGTT AA^CGTT AgeI 5141 ACCGGT A^CCGGT AhdI 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 5125 GGGCCC GGGCC^C AspEI 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1867 CACGTG CAC^GTG BciVI 762 GTATCC GTATCC(6/5) BclI 5792 TGATCA T^GATCA BsmI 3541 GAATGC GAATGC(1/-1) BspDI 208 ATCGAT AT^CGAT BsrDI 5443 CATTGC GCAATG(2/0) BstEII 2477 GGTGACC G^GT[ACGT]ACC BstPI 2477 GGTGACC G^GT[ACGT]ACC ClaI 208 ATCGAT AT^CGAT Eam1105I 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2477 GGTGACC G^GT[ACGT]ACC EcoT22I 4887 ATGCAT ATGCA^T FbaI 5792 TGATCA T^GATCA HindIII 2110 AAGCTT A^AGCTT NdeI 2383 CATATG CA^TATG NsiI 4887 ATGCAT ATGCA^T PaeR7I 555 CTCGAG C^TCGAG PflFI 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3770 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 5141 ACCGGT A^CCGGT PmaCI 1867 CACGTG CAC^GTG PmlI 1867 CACGTG CAC^GTG Psp1406I 4748 AACGTT AA^CGTT SbfI 1706 CCTGCAGG CCTGCA^GG ScaI 4814 AGTACT AGT^ACT SphI 2623 GCATGC GCATG^C Sse8387I 1706 CCTGCAGG CCTGCA^GG SspI 6474 AATATT AAT^ATT Tth111I 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3770 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 555 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 1251 GGTACC G^GTACC 6378 GGTACC Aor51HI 1644 AGCGCT AGC^GCT 2122 AGCGCT Asp700I 376 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1970 GAAGCAGTTC Asp718I 1251 GGTACC G^GTACC 6378 GGTACC AviII 880 TGCGCA TGC^GCA 1792 TGCGCA AvrII 66 CCTAGG C^CTAGG 3905 CCTAGG BamHI 2341 GGATCC G^GATCC 6073 GGATCC BbeI 893 GGCGCC GGCGC^C 3382 GGCGCC BcgI 1376 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2602 GCAAGGCAGTCG BlnI 66 CCTAGG C^CTAGG 3905 CCTAGG BsaAI 1867 CACGTG [CT]AC^GT[GA] 2588 CACGTA BspHI 1258 TCATGA T^CATGA 5390 TCATGA BsrBI 220 GAGCGG CCGCTC(-3/-3) 1199 GAGCGG BssHII 41 GCGCGC G^CGCGC 129 GCGCGC DraI 4136 TTTAAA TTT^AAA 4715 TTTAAA EagI 356 CGGCCG C^GGCCG 4283 CGGCCG EclXI 356 CGGCCG C^GGCCG 4283 CGGCCG Eco47III 1644 AGCGCT AGC^GCT 2122 AGCGCT Eco52I 356 CGGCCG C^GGCCG 4283 CGGCCG FspI 880 TGCGCA TGC^GCA 1792 TGCGCA KasI 893 GGCGCC G^GCGCC 3382 GGCGCC KpnI 1251 GGTACC GGTAC^C 6378 GGTACC NaeI 2464 GCCGGC GCC^GGC 3829 GCCGGC NarI 893 GGCGCC GG^CGCC 3382 GGCGCC NcoI 2660 CCATGG C^CATGG 4755 CCATGG NgoMIV 2464 GCCGGC G^CCGGC 3829 GCCGGC NheI 5668 GCTAGC G^CTAGC 5994 GCTAGC RcaI 1258 TCATGA T^CATGA 5390 TCATGA SapI 2576 GAAGAGC GCTCTTC(1/4) 2468 GCTCTTC SfoI 893 GGCGCC GGC^GCC 3382 GGCGCC SmaI 520 CCCGGG CCC^GGG 1206 CCCGGG XmaCI 520 CCCGGG C^CCGGG 1206 CCCGGG XmaI 520 CCCGGG C^CCGGG 1206 CCCGGG XmnI 376 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1970 GAAGCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspLU11I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII