##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AcsI AflII ApoI AscI
AseI AsnI BfrI BglII BsaBI
BsiWI BsmBI BssSI Bst1107I BstBI
BstZ17I CpoI EcoRI EcoRV FseI
HincII HindII HpaI MamI MfeI
MluI MunI NotI NruI NspV
PacI PmeI PshAI PshBI PvuI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3930 GTCTAC GT^[AC][GT]AC
AclI 4748 AACGTT AA^CGTT
AgeI 5141 ACCGGT A^CCGGT
AhdI 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 5125 GGGCCC GGGCC^C
AspEI 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1867 CACGTG CAC^GTG
BciVI 762 GTATCC GTATCC(6/5)
BclI 5792 TGATCA T^GATCA
BsmI 3541 GAATGC GAATGC(1/-1)
BspDI 208 ATCGAT AT^CGAT
BsrDI 5443 CATTGC GCAATG(2/0)
BstEII 2477 GGTGACC G^GT[ACGT]ACC
BstPI 2477 GGTGACC G^GT[ACGT]ACC
ClaI 208 ATCGAT AT^CGAT
Eam1105I 2877 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2477 GGTGACC G^GT[ACGT]ACC
EcoT22I 4887 ATGCAT ATGCA^T
FbaI 5792 TGATCA T^GATCA
HindIII 2110 AAGCTT A^AGCTT
NdeI 2383 CATATG CA^TATG
NsiI 4887 ATGCAT ATGCA^T
PaeR7I 555 CTCGAG C^TCGAG
PflFI 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3770 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 5141 ACCGGT A^CCGGT
PmaCI 1867 CACGTG CAC^GTG
PmlI 1867 CACGTG CAC^GTG
Psp1406I 4748 AACGTT AA^CGTT
SbfI 1706 CCTGCAGG CCTGCA^GG
ScaI 4814 AGTACT AGT^ACT
SphI 2623 GCATGC GCATG^C
Sse8387I 1706 CCTGCAGG CCTGCA^GG
SspI 6474 AATATT AAT^ATT
Tth111I 2373 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3770 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 555 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1251 GGTACC G^GTACC
6378 GGTACC
Aor51HI 1644 AGCGCT AGC^GCT
2122 AGCGCT
Asp700I 376 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1970 GAAGCAGTTC
Asp718I 1251 GGTACC G^GTACC
6378 GGTACC
AviII 880 TGCGCA TGC^GCA
1792 TGCGCA
AvrII 66 CCTAGG C^CTAGG
3905 CCTAGG
BamHI 2341 GGATCC G^GATCC
6073 GGATCC
BbeI 893 GGCGCC GGCGC^C
3382 GGCGCC
BcgI 1376 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2602 GCAAGGCAGTCG
BlnI 66 CCTAGG C^CTAGG
3905 CCTAGG
BsaAI 1867 CACGTG [CT]AC^GT[GA]
2588 CACGTA
BspHI 1258 TCATGA T^CATGA
5390 TCATGA
BsrBI 220 GAGCGG CCGCTC(-3/-3)
1199 GAGCGG
BssHII 41 GCGCGC G^CGCGC
129 GCGCGC
DraI 4136 TTTAAA TTT^AAA
4715 TTTAAA
EagI 356 CGGCCG C^GGCCG
4283 CGGCCG
EclXI 356 CGGCCG C^GGCCG
4283 CGGCCG
Eco47III 1644 AGCGCT AGC^GCT
2122 AGCGCT
Eco52I 356 CGGCCG C^GGCCG
4283 CGGCCG
FspI 880 TGCGCA TGC^GCA
1792 TGCGCA
KasI 893 GGCGCC G^GCGCC
3382 GGCGCC
KpnI 1251 GGTACC GGTAC^C
6378 GGTACC
NaeI 2464 GCCGGC GCC^GGC
3829 GCCGGC
NarI 893 GGCGCC GG^CGCC
3382 GGCGCC
NcoI 2660 CCATGG C^CATGG
4755 CCATGG
NgoMIV 2464 GCCGGC G^CCGGC
3829 GCCGGC
NheI 5668 GCTAGC G^CTAGC
5994 GCTAGC
RcaI 1258 TCATGA T^CATGA
5390 TCATGA
SapI 2576 GAAGAGC GCTCTTC(1/4)
2468 GCTCTTC
SfoI 893 GGCGCC GGC^GCC
3382 GGCGCC
SmaI 520 CCCGGG CCC^GGG
1206 CCCGGG
XmaCI 520 CCCGGG C^CCGGG
1206 CCCGGG
XmaI 520 CCCGGG C^CCGGG
1206 CCCGGG
XmnI 376 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1970 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspLU11I BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII