################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AspI AviII BbrPI BglI BsiEI BsiWI BsrFI BstBI Bsu36I Cfr10I DrdI EagI EclXI Eco47III Eco52I Eco81I FseI FspI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PshAI PvuI SacI SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 1622 TCCGGA T^CCGGA AclI 5626 AACGTT AA^CGTT AcyI 414 GGCGCC G[GA]^CG[CT]C AflII 4314 CTTAAG C^TTAAG AflIII 4806 ACATGT A^C[GA][CT]GT AseI 4097 ATTAAT AT^TAAT AsnI 4097 ATTAAT AT^TAAT AvrII 5079 CCTAGG C^CTAGG BbeI 414 GGCGCC GGCGC^C BciVI 1247 GGATAC GTATCC(6/5) BfrI 4314 CTTAAG C^TTAAG BglII 5046 AGATCT A^GATCT BlnI 5079 CCTAGG C^CTAGG BsaAI 1426 CACGTA [CT]AC^GT[GA] BsaHI 414 GGCGCC G[GA]^CG[CT]C BsaI 5025 GGTCTC GGTCTC(1/5) BseAI 1622 TCCGGA T^CCGGA BsmBI 533 GAGACG CGTCTC(1/5) BspDI 2440 ATCGAT AT^CGAT BspEI 1622 TCCGGA T^CCGGA BspHI 5041 TCATGA T^CATGA BspLU11I 4806 ACATGT A^CATGT BssHII 361 GCGCGC G^CGCGC BstAPI 4476 GCACGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 2440 ATCGAT AT^CGAT CpoI 3625 CGGACCG CG^G[AT]CCG EcoNI 2061 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2633 GATATC GAT^ATC Hin1I 414 GGCGCC G[GA]^CG[CT]C HindIII 5467 AAGCTT A^AGCTT KasI 414 GGCGCC G^GCGCC KspI 523 CCGCGG CCGC^GG MroI 1622 TCCGGA T^CCGGA NarI 414 GGCGCC GG^CGCC PflMI 4578 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 860 GTTTAAAC GTTT^AAAC PshBI 4097 ATTAAT AT^TAAT Psp1406I 5626 AACGTT AA^CGTT RcaI 5041 TCATGA T^CATGA RsrII 3625 CGGACCG CG^G[AT]CCG SacII 523 CCGCGG CCGC^GG SapI 700 GCTCTTC GCTCTTC(1/4) SfoI 414 GGCGCC GGC^GCC StuI 639 AGGCCT AGG^CCT SwaI 3930 ATTTAAAT ATTT^AAAT Van91I 4578 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 492 GGTACC G^GTACC 3189 GGTACC AhdI 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3627 GACCGCCTGTC Asp700I 1852 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5429 GAAACCTTTC Asp718I 492 GGTACC G^GTACC 3189 GGTACC AspEI 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3627 GACCGCCTGTC BamHI 777 GGATCC G^GATCC 1372 GGATCC BclI 2391 TGATCA T^GATCA 5285 TGATCA BlpI 544 GCTGAGC GC^T[ACGT]AGC 969 GCTGAGC Bpu1102I 544 GCTGAGC GC^T[ACGT]AGC 969 GCTGAGC BsaBI 1971 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4719 GATATGAATC Bsp1407I 1892 TGTACA T^GTACA 5232 TGTACA BsrGI 1892 TGTACA T^GTACA 5232 TGTACA BssSI 3586 CACGAG CACGAG(-5/-1) 385 CTCGTG CelII 544 GCTGAGC GC^T[ACGT]AGC 969 GCTGAGC DraIII 346 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4477 CACGATGTG Eam1105I 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3627 GACCGCCTGTC FbaI 2391 TGATCA T^GATCA 5285 TGATCA HaeII 414 GGCGCC [GA]GCGC^[CT] 2143 GGCGCT HpaI 2521 GTTAAC GTT^AAC 4869 GTTAAC KpnI 492 GGTACC GGTAC^C 3189 GGTACC MamI 1971 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4719 GATATGAATC NcoI 1301 CCATGG C^CATGG 1899 CCATGG NdeI 1553 CATATG CA^TATG 5115 CATATG PvuII 2265 CAGCTG CAG^CTG 5093 CAGCTG ScaI 4159 AGTACT AGT^ACT 4436 AGTACT SspBI 1892 TGTACA T^GTACA 5232 TGTACA SspI 3945 AATATT AAT^ATT 4753 AATATT XmnI 1852 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5429 GAAACCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI Bst1107I BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII