##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspI AviII
BbrPI BglI BsiEI BsiWI BsrFI
BstBI Bsu36I Cfr10I DrdI EagI
EclXI Eco47III Eco52I Eco81I FseI
FspI MluI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI PshAI
PvuI SacI SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 1622 TCCGGA T^CCGGA
AclI 5626 AACGTT AA^CGTT
AcyI 414 GGCGCC G[GA]^CG[CT]C
AflII 4314 CTTAAG C^TTAAG
AflIII 4806 ACATGT A^C[GA][CT]GT
AseI 4097 ATTAAT AT^TAAT
AsnI 4097 ATTAAT AT^TAAT
AvrII 5079 CCTAGG C^CTAGG
BbeI 414 GGCGCC GGCGC^C
BciVI 1247 GGATAC GTATCC(6/5)
BfrI 4314 CTTAAG C^TTAAG
BglII 5046 AGATCT A^GATCT
BlnI 5079 CCTAGG C^CTAGG
BsaAI 1426 CACGTA [CT]AC^GT[GA]
BsaHI 414 GGCGCC G[GA]^CG[CT]C
BsaI 5025 GGTCTC GGTCTC(1/5)
BseAI 1622 TCCGGA T^CCGGA
BsmBI 533 GAGACG CGTCTC(1/5)
BspDI 2440 ATCGAT AT^CGAT
BspEI 1622 TCCGGA T^CCGGA
BspHI 5041 TCATGA T^CATGA
BspLU11I 4806 ACATGT A^CATGT
BssHII 361 GCGCGC G^CGCGC
BstAPI 4476 GCACGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2440 ATCGAT AT^CGAT
CpoI 3625 CGGACCG CG^G[AT]CCG
EcoNI 2061 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2633 GATATC GAT^ATC
Hin1I 414 GGCGCC G[GA]^CG[CT]C
HindIII 5467 AAGCTT A^AGCTT
KasI 414 GGCGCC G^GCGCC
KspI 523 CCGCGG CCGC^GG
MroI 1622 TCCGGA T^CCGGA
NarI 414 GGCGCC GG^CGCC
PflMI 4578 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 860 GTTTAAAC GTTT^AAAC
PshBI 4097 ATTAAT AT^TAAT
Psp1406I 5626 AACGTT AA^CGTT
RcaI 5041 TCATGA T^CATGA
RsrII 3625 CGGACCG CG^G[AT]CCG
SacII 523 CCGCGG CCGC^GG
SapI 700 GCTCTTC GCTCTTC(1/4)
SfoI 414 GGCGCC GGC^GCC
StuI 639 AGGCCT AGG^CCT
SwaI 3930 ATTTAAAT ATTT^AAAT
Van91I 4578 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 492 GGTACC G^GTACC
3189 GGTACC
AhdI 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3627 GACCGCCTGTC
Asp700I 1852 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5429 GAAACCTTTC
Asp718I 492 GGTACC G^GTACC
3189 GGTACC
AspEI 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3627 GACCGCCTGTC
BamHI 777 GGATCC G^GATCC
1372 GGATCC
BclI 2391 TGATCA T^GATCA
5285 TGATCA
BlpI 544 GCTGAGC GC^T[ACGT]AGC
969 GCTGAGC
Bpu1102I 544 GCTGAGC GC^T[ACGT]AGC
969 GCTGAGC
BsaBI 1971 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4719 GATATGAATC
Bsp1407I 1892 TGTACA T^GTACA
5232 TGTACA
BsrGI 1892 TGTACA T^GTACA
5232 TGTACA
BssSI 3586 CACGAG CACGAG(-5/-1)
385 CTCGTG
CelII 544 GCTGAGC GC^T[ACGT]AGC
969 GCTGAGC
DraIII 346 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4477 CACGATGTG
Eam1105I 3476 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3627 GACCGCCTGTC
FbaI 2391 TGATCA T^GATCA
5285 TGATCA
HaeII 414 GGCGCC [GA]GCGC^[CT]
2143 GGCGCT
HpaI 2521 GTTAAC GTT^AAC
4869 GTTAAC
KpnI 492 GGTACC GGTAC^C
3189 GGTACC
MamI 1971 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4719 GATATGAATC
NcoI 1301 CCATGG C^CATGG
1899 CCATGG
NdeI 1553 CATATG CA^TATG
5115 CATATG
PvuII 2265 CAGCTG CAG^CTG
5093 CAGCTG
ScaI 4159 AGTACT AGT^ACT
4436 AGTACT
SspBI 1892 TGTACA T^GTACA
5232 TGTACA
SspI 3945 AATATT AAT^ATT
4753 AATATT
XmnI 1852 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5429 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI Bst1107I BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII