##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BfrI BglII BlnI
BsaBI BseAI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI Bst1107I
BstZ17I Cfr10I ClaI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshBI Psp1406I
SacII SalI SapI SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 5102 GACGTC GACGT^C
Asp700I 2652 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4987 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4700 GGCGCC GGCGC^C
BclI 2318 TGATCA T^GATCA
BsiEI 2768 CGATCG CG[GA][CT]^CG
BsmBI 4695 GAGACG CGTCTC(1/5)
BspHI 1634 TCATGA T^CATGA
BstBI 1298 TTCGAA TT^CGAA
BstYI 3174 GGATCT [GA]^GATC[CT]
CpoI 4673 CGGTCCG CG^G[AT]CCG
DrdI 1719 GACTCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2318 TGATCA T^GATCA
KasI 4700 GGCGCC G^GCGCC
MflI 3174 GGATCT [GA]^GATC[CT]
NarI 4700 GGCGCC GG^CGCC
NheI 2479 GCTAGC G^CTAGC
NspI 180 GCATGT [GA]CATG^[CT]
NspV 1298 TTCGAA TT^CGAA
PflFI 4987 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4056 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2768 CGATCG CGAT^CG
RcaI 1634 TCATGA T^CATGA
RsrII 4673 CGGTCCG CG^G[AT]CCG
ScaI 3395 AGTACT AGT^ACT
SfoI 4700 GGCGCC GGC^GCC
SfuI 1298 TTCGAA TT^CGAA
SmaI 5044 CCCGGG CCC^GGG
SspI 3999 AATATT AAT^ATT
StuI 2796 AGGCCT AGG^CCT
Tth111I 4987 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3174 GGATCT [GA]^GATC[CT]
XmaCI 5044 CCCGGG C^CCGGG
XmaI 5044 CCCGGG C^CCGGG
XmnI 2652 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 753 ACACGT A^C[GA][CT]GT
5010 ACGTGT
BbrPI 754 CACGTG CAC^GTG
5009 CACGTG
BciVI 2638 GGATAC GTATCC(6/5)
2105 GTATCC
BsiWI 192 CGTACG C^GTACG
4636 CGTACG
BsrBI 1006 CCGCTC CCGCTC(-3/-3)
2258 GAGCGG
BsrDI 3598 CATTGC GCAATG(2/0)
3705 CATTGC
BssHII 4625 GCGCGC G^CGCGC
4764 GCGCGC
BssSI 150 CTCGTG CACGAG(-5/-1)
4941 CTCGTG
BstEII 450 GGTGACC G^GT[ACGT]ACC
1528 GGTAACC
BstPI 450 GGTGACC G^GT[ACGT]ACC
1528 GGTAACC
EcoNI 297 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3974 CCTAGTGCAGG
EcoO65I 450 GGTGACC G^GT[ACGT]ACC
1528 GGTAACC
HincII 1728 GTCAAC GT[CT]^[GA]AC
4961 GTCAAC
HindII 1728 GTCAAC GT[CT]^[GA]AC
4961 GTCAAC
NcoI 2462 CCATGG C^CATGG
2717 CCATGG
PmaCI 754 CACGTG CAC^GTG
5009 CACGTG
PmlI 754 CACGTG CAC^GTG
5009 CACGTG
SacI 2137 GAGCTC GAGCT^C
4751 GAGCTC
SpeI 3717 ACTAGT A^CTAGT
4373 ACTAGT
XbaI 502 TCTAGA T^CTAGA
1722 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI