##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AgeI Alw44I Aor51HI ApaLI
AscI AviII BamHI BglI BglII
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I FseI
FspI KspI MluI NotI NruI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3299 GACGTC GACGT^C
Acc65I 3305 GGTACC G^GTACC
AccII 5089 CGCG CG^CG
AccIII 4393 TCCGGA T^CCGGA
AflII 2821 CTTAAG C^TTAAG
ApaI 3758 GGGCCC GGGCC^C
Asp718I 3305 GGTACC G^GTACC
AspI 4294 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1009 CCTAGG C^CTAGG
BbeI 51 GGCGCC GGCGC^C
BbrPI 5691 CACGTG CAC^GTG
BcgI 1402 GCACAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3671 GGATAC GTATCC(6/5)
BclI 1969 TGATCA T^GATCA
BfrI 2821 CTTAAG C^TTAAG
BlnI 1009 CCTAGG C^CTAGG
BsaAI 5691 CACGTG [CT]AC^GT[GA]
BsaI 2381 GAGACC GGTCTC(1/5)
BseAI 4393 TCCGGA T^CCGGA
BspEI 4393 TCCGGA T^CCGGA
BspHI 3243 TCATGA T^CATGA
BssHII 5088 GCGCGC G^CGCGC
Bst1107I 1136 GTATAC GTA^TAC
BstEII 1394 GGTTACC G^GT[ACGT]ACC
BstPI 1394 GGTTACC G^GT[ACGT]ACC
BstUI 5089 CGCG CG^CG
BstZ17I 1136 GTATAC GTA^TAC
DraIII 1777 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1394 GGTTACC G^GT[ACGT]ACC
EcoT22I 1089 ATGCAT ATGCA^T
FbaI 1969 TGATCA T^GATCA
HaeII 51 GGCGCC [GA]GCGC^[CT]
KasI 51 GGCGCC G^GCGCC
KpnI 3305 GGTACC GGTAC^C
MroI 4393 TCCGGA T^CCGGA
MvnI 5089 CGCG CG^CG
NaeI 4237 GCCGGC GCC^GGC
NarI 51 GGCGCC GG^CGCC
NgoMIV 4237 GCCGGC G^CCGGC
NheI 704 GCTAGC G^CTAGC
NsiI 1089 ATGCAT ATGCA^T
PacI 5823 TTAATTAA TTAAT^TAA
PaeR7I 1202 CTCGAG C^TCGAG
PflFI 4294 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5691 CACGTG CAC^GTG
PmlI 5691 CACGTG CAC^GTG
RcaI 3243 TCATGA T^CATGA
SexAI 3091 ACCAGGT A^CC[AT]GGT
SfoI 51 GGCGCC GGC^GCC
Tth111I 4294 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2638 TCTAGA T^CTAGA
XcmI 1723 CCAGCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1202 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 51 GGCGCC G[GA]^CG[CT]C
3299 GACGTC
AflIII 1713 ACATGT A^C[GA][CT]GT
6348 ACATGT
AocI 2487 CCTCAGG CC^T[ACGT]AGG
3775 CCTAAGG
BbvCI 190 CCTCAGC CCTCAGC(-5/-2)
4571 CCTCAGC
BsaBI 599 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1289 GATGACAATC
BsaHI 51 GGCGCC G[GA]^CG[CT]C
3299 GACGTC
BsaWI 4393 TCCGGA [AT]^CCGG[AT]
5586 ACCGGA
BspLU11I 1713 ACATGT A^CATGT
6348 ACATGT
BsrFI 2290 GCCGGT [GA]^CCGG[CT]
4237 GCCGGC
BssSI 3842 CACGAG CACGAG(-5/-1)
314 CTCGTG
BstBI 569 TTCGAA TT^CGAA
1410 TTCGAA
BstXI 3639 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3804 CCACCGAGATGG
Bsu36I 2487 CCTCAGG CC^T[ACGT]AGG
3775 CCTAAGG
Cfr10I 2290 GCCGGT [GA]^CCGG[CT]
4237 GCCGGC
Eco81I 2487 CCTCAGG CC^T[ACGT]AGG
3775 CCTAAGG
EcoRV 2139 GATATC GAT^ATC
5979 GATATC
Hin1I 51 GGCGCC G[GA]^CG[CT]C
3299 GACGTC
HindIII 2520 AAGCTT A^AGCTT
3535 AAGCTT
HpaI 713 GTTAAC GTT^AAC
1270 GTTAAC
MamI 599 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1289 GATGACAATC
MfeI 629 CAATTG C^AATTG
1320 CAATTG
MunI 629 CAATTG C^AATTG
1320 CAATTG
NdeI 4482 CATATG CA^TATG
4826 CATATG
NspV 569 TTCGAA TT^CGAA
1410 TTCGAA
PstI 532 CTGCAG CTGCA^G
1354 CTGCAG
SapI 584 GAAGAGC GCTCTTC(1/4)
4858 GAAGAGC
SfuI 569 TTCGAA TT^CGAA
1410 TTCGAA
SmaI 4563 CCCGGG CCC^GGG
4784 CCCGGG
XmaCI 4563 CCCGGG C^CCGGG
4784 CCCGGG
XmaI 4563 CCCGGG C^CCGGG
4784 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI