##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII BclI BsaWI BseAI BsiWI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I EcoRI EcoRV
FbaI FseI FspI KpnI KspI
MluI MroI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfuI SgrAI
SnaBI Sse8387I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 956 CTTAAG C^TTAAG
Aor51HI 5383 AGCGCT AGC^GCT
AspI 875 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1190 CCTAGG C^CTAGG
BbeI 4625 GGCGCC GGCGC^C
BcgI 971 GCAACTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5754 GGATAC GTATCC(6/5)
BfrI 956 CTTAAG C^TTAAG
BlnI 1190 CCTAGG C^CTAGG
BsaBI 6312 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2178 GCATTC GAATGC(1/-1)
BspLU11I 5575 ACATGT A^CATGT
DrdI 5320 GACACTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5383 AGCGCT AGC^GCT
HincII 1332 GTTAAC GT[CT]^[GA]AC
HindII 1332 GTTAAC GT[CT]^[GA]AC
HpaI 1332 GTTAAC GTT^AAC
KasI 4625 GGCGCC G^GCGCC
MamI 6312 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6517 CAATTG C^AATTG
MunI 6517 CAATTG C^AATTG
NarI 4625 GGCGCC GG^CGCC
NheI 4276 GCTAGC G^CTAGC
PflFI 875 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 2887 ACCTGGT A^CC[AT]GGT
SfoI 4625 GGCGCC GGC^GCC
SmaI 5927 CCCGGG CCC^GGG
SpeI 1349 ACTAGT A^CTAGT
SphI 382 GCATGC GCATG^C
SspI 6666 AATATT AAT^ATT
SwaI 6313 ATTTAAAT ATTT^AAAT
Tth111I 875 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 5927 CCCGGG C^CCGGG
XmaI 5927 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 54 GTCTAC GT^[AC][GT]AC
663 GTCTAC
AflIII 3946 ACGTGT A^C[GA][CT]GT
5575 ACATGT
AocI 1685 CCTGAGG CC^T[ACGT]AGG
2051 CCTGAGG
Asp700I 1089 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6712 GAAATTATTC
BamHI 2911 GGATCC G^GATCC
5006 GGATCC
BbrPI 3945 CACGTG CAC^GTG
6082 CACGTG
BglII 1318 AGATCT A^GATCT
6225 AGATCT
BsaAI 3945 CACGTG [CT]AC^GT[GA]
6082 CACGTG
BsmBI 2291 CGTCTC CGTCTC(1/5)
3174 GAGACG
Bsp1407I 2938 TGTACA T^GTACA
4487 TGTACA
BspHI 2245 TCATGA T^CATGA
3999 TCATGA
BsrGI 2938 TGTACA T^GTACA
4487 TGTACA
BssSI 1101 CACGAG CACGAG(-5/-1)
456 CTCGTG
BstEII 1664 GGTAACC G^GT[ACGT]ACC
5950 GGTCACC
BstPI 1664 GGTAACC G^GT[ACGT]ACC
5950 GGTCACC
Bsu36I 1685 CCTGAGG CC^T[ACGT]AGG
2051 CCTGAGG
Eco81I 1685 CCTGAGG CC^T[ACGT]AGG
2051 CCTGAGG
EcoO65I 1664 GGTAACC G^GT[ACGT]ACC
5950 GGTCACC
EcoT22I 6165 ATGCAT ATGCA^T
6257 ATGCAT
HindIII 1739 AAGCTT A^AGCTT
6378 AAGCTT
NsiI 6165 ATGCAT ATGCA^T
6257 ATGCAT
PmaCI 3945 CACGTG CAC^GTG
6082 CACGTG
PmlI 3945 CACGTG CAC^GTG
6082 CACGTG
RcaI 2245 TCATGA T^CATGA
3999 TCATGA
SapI 2994 GCTCTTC GCTCTTC(1/4)
5859 GCTCTTC
ScaI 340 AGTACT AGT^ACT
6604 AGTACT
SspBI 2938 TGTACA T^GTACA
4487 TGTACA
XmnI 1089 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6712 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII