##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BglII BsiWI BsmI
Bsp1407I BspDI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eco47III
EcoRV FseI FspI KpnI MluI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2252 GTCTAC GT^[AC][GT]AC
AccIII 1875 TCCGGA T^CCGGA
AhdI 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2539 GTGCAC G^TGCAC
ApaI 323 GGGCCC GGGCC^C
ApaLI 2539 GTGCAC G^TGCAC
AspEI 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3414 CCTAGG C^CTAGG
BalI 3487 TGGCCA TGG^CCA
BamHI 827 GGATCC G^GATCC
BciVI 2221 GTATCC GTATCC(6/5)
BclI 4055 TGATCA T^GATCA
BlnI 3414 CCTAGG C^CTAGG
BsaBI 2523 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1875 TCCGGA T^CCGGA
BspEI 1875 TCCGGA T^CCGGA
BssHII 467 GCGCGC G^CGCGC
BssSI 3073 CTCGTG CACGAG(-5/-1)
Eam1105I 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2099 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4927 ATGCAT ATGCA^T
FbaI 4055 TGATCA T^GATCA
HindIII 3809 AAGCTT A^AGCTT
HpaI 2295 GTTAAC GTT^AAC
KspI 110 CCGCGG CCGC^GG
MamI 2523 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1274 CAATTG C^AATTG
MluNI 3487 TGGCCA TGG^CCA
MroI 1875 TCCGGA T^CCGGA
MscI 3487 TGGCCA TGG^CCA
MunI 1274 CAATTG C^AATTG
NdeI 3845 CATATG CA^TATG
NheI 2645 GCTAGC G^CTAGC
NsiI 4927 ATGCAT ATGCA^T
PaeR7I 1912 CTCGAG C^TCGAG
PflMI 526 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 110 CCGCGG CCGC^GG
SapI 4164 GCTCTTC GCTCTTC(1/4)
SpeI 5181 ACTAGT A^CTAGT
Van91I 526 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 836 TCTAGA T^CTAGA
XhoI 1912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 1826 CCTGAGG CC^T[ACGT]AGG
3649 CCTCAGG
BbrPI 568 CACGTG CAC^GTG
2405 CACGTG
BbvCI 613 CCTCAGC CCTCAGC(-5/-2)
2349 GCTGAGG
BcgI 2404 GCACGTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3684 GCACCCACCTCG
BglI 109 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3195 GCCTTTGCGGC
BpmI 1415 CTCCAG CTGGAG(16/14)
3963 CTCCAG
BsaI 3989 GAGACC GGTCTC(1/5)
1198 GGTCTC
BsaWI 709 TCCGGT [AT]^CCGG[AT]
1875 TCCGGA
BsmBI 103 CGTCTC CGTCTC(1/5)
1632 GAGACG
BspHI 1163 TCATGA T^CATGA
5156 TCATGA
BspLU11I 4575 ACATGT A^CATGT
5094 ACATGT
BsrDI 815 CATTGC GCAATG(2/0)
1928 GCAATG
BsrFI 66 GCCGGC [GA]^CCGG[CT]
1373 GCCGGC
BstAPI 3192 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4929 GCATAAAGTGC
BstEII 4208 GGTCACC G^GT[ACGT]ACC
4408 GGTCACC
BstPI 4208 GGTCACC G^GT[ACGT]ACC
4408 GGTCACC
Bsu36I 1826 CCTGAGG CC^T[ACGT]AGG
3649 CCTCAGG
Cfr10I 66 GCCGGC [GA]^CCGG[CT]
1373 GCCGGC
DraI 4898 TTTAAA TTT^AAA
5030 TTTAAA
DraIII 2542 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2637 CACCCTGTG
Eco81I 1826 CCTGAGG CC^T[ACGT]AGG
3649 CCTCAGG
EcoO65I 4208 GGTCACC G^GT[ACGT]ACC
4408 GGTCACC
EcoRI 2041 GAATTC G^AATTC
3393 GAATTC
NaeI 66 GCCGGC GCC^GGC
1373 GCCGGC
NgoMIV 66 GCCGGC G^CCGGC
1373 GCCGGC
PmaCI 568 CACGTG CAC^GTG
2405 CACGTG
PmlI 568 CACGTG CAC^GTG
2405 CACGTG
RcaI 1163 TCATGA T^CATGA
5156 TCATGA
SacI 12 GAGCTC GAGCT^C
3020 GAGCTC
SmaI 162 CCCGGG CCC^GGG
548 CCCGGG
SphI 2689 GCATGC GCATG^C
3209 GCATGC
XcmI 3313 CCACCCCTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3511 CCACACCAGAGCTGG
XmaCI 162 CCCGGG C^CCGGG
548 CCCGGG
XmaI 162 CCCGGG C^CCGGG
548 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BfaI BfrI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII