################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII Acc65I AclI AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII BglII BsiWI BsmI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eco47III EcoRV FseI FspI KpnI MluI NotI NruI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspBI StuI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 2252 GTCTAC GT^[AC][GT]AC AccIII 1875 TCCGGA T^CCGGA AhdI 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2539 GTGCAC G^TGCAC ApaI 323 GGGCCC GGGCC^C ApaLI 2539 GTGCAC G^TGCAC AspEI 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3414 CCTAGG C^CTAGG BalI 3487 TGGCCA TGG^CCA BamHI 827 GGATCC G^GATCC BciVI 2221 GTATCC GTATCC(6/5) BclI 4055 TGATCA T^GATCA BlnI 3414 CCTAGG C^CTAGG BsaBI 2523 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1875 TCCGGA T^CCGGA BspEI 1875 TCCGGA T^CCGGA BssHII 467 GCGCGC G^CGCGC BssSI 3073 CTCGTG CACGAG(-5/-1) Eam1105I 634 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2099 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 4927 ATGCAT ATGCA^T FbaI 4055 TGATCA T^GATCA HindIII 3809 AAGCTT A^AGCTT HpaI 2295 GTTAAC GTT^AAC KspI 110 CCGCGG CCGC^GG MamI 2523 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1274 CAATTG C^AATTG MluNI 3487 TGGCCA TGG^CCA MroI 1875 TCCGGA T^CCGGA MscI 3487 TGGCCA TGG^CCA MunI 1274 CAATTG C^AATTG NdeI 3845 CATATG CA^TATG NheI 2645 GCTAGC G^CTAGC NsiI 4927 ATGCAT ATGCA^T PaeR7I 1912 CTCGAG C^TCGAG PflMI 526 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 110 CCGCGG CCGC^GG SapI 4164 GCTCTTC GCTCTTC(1/4) SpeI 5181 ACTAGT A^CTAGT Van91I 526 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 836 TCTAGA T^CTAGA XhoI 1912 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AocI 1826 CCTGAGG CC^T[ACGT]AGG 3649 CCTCAGG BbrPI 568 CACGTG CAC^GTG 2405 CACGTG BbvCI 613 CCTCAGC CCTCAGC(-5/-2) 2349 GCTGAGG BcgI 2404 GCACGTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3684 GCACCCACCTCG BglI 109 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3195 GCCTTTGCGGC BpmI 1415 CTCCAG CTGGAG(16/14) 3963 CTCCAG BsaI 3989 GAGACC GGTCTC(1/5) 1198 GGTCTC BsaWI 709 TCCGGT [AT]^CCGG[AT] 1875 TCCGGA BsmBI 103 CGTCTC CGTCTC(1/5) 1632 GAGACG BspHI 1163 TCATGA T^CATGA 5156 TCATGA BspLU11I 4575 ACATGT A^CATGT 5094 ACATGT BsrDI 815 CATTGC GCAATG(2/0) 1928 GCAATG BsrFI 66 GCCGGC [GA]^CCGG[CT] 1373 GCCGGC BstAPI 3192 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4929 GCATAAAGTGC BstEII 4208 GGTCACC G^GT[ACGT]ACC 4408 GGTCACC BstPI 4208 GGTCACC G^GT[ACGT]ACC 4408 GGTCACC Bsu36I 1826 CCTGAGG CC^T[ACGT]AGG 3649 CCTCAGG Cfr10I 66 GCCGGC [GA]^CCGG[CT] 1373 GCCGGC DraI 4898 TTTAAA TTT^AAA 5030 TTTAAA DraIII 2542 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2637 CACCCTGTG Eco81I 1826 CCTGAGG CC^T[ACGT]AGG 3649 CCTCAGG EcoO65I 4208 GGTCACC G^GT[ACGT]ACC 4408 GGTCACC EcoRI 2041 GAATTC G^AATTC 3393 GAATTC NaeI 66 GCCGGC GCC^GGC 1373 GCCGGC NgoMIV 66 GCCGGC G^CCGGC 1373 GCCGGC PmaCI 568 CACGTG CAC^GTG 2405 CACGTG PmlI 568 CACGTG CAC^GTG 2405 CACGTG RcaI 1163 TCATGA T^CATGA 5156 TCATGA SacI 12 GAGCTC GAGCT^C 3020 GAGCTC SmaI 162 CCCGGG CCC^GGG 548 CCCGGG SphI 2689 GCATGC GCATG^C 3209 GCATGC XcmI 3313 CCACCCCTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3511 CCACACCAGAGCTGG XmaCI 162 CCCGGG C^CCGGG 548 CCCGGG XmaI 162 CCCGGG C^CCGGG 548 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BfrI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII