##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccIII AgeI AscI AseI
AsnI AviII AvrII BbeI BclI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssHII BstEII BstPI ClaI
CpoI EagI EclXI Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
KasI KspI MamI MluI MroI
NarI NotI NruI NsiI PacI
PinAI PmeI PshAI PshBI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI Sse8387I SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 206 CGCG CG^CG
AclI 623 AACGTT AA^CGTT
AcyI 3626 GGCGTC G[GA]^CG[CT]C
Aor51HI 6245 AGCGCT AGC^GCT
Asp700I 5729 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1216 CACGTG CAC^GTG
BcgI 5977 GCAAATAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1665 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1665 GCTCAGC GC^T[ACGT]AGC
BsaHI 3626 GGCGTC G[GA]^CG[CT]C
Bsp1407I 6289 TGTACA T^GTACA
BspHI 2039 TCATGA T^CATGA
BsrBI 329 GAGCGG CCGCTC(-3/-3)
BsrFI 1903 GCCGGC [GA]^CCGG[CT]
BsrGI 6289 TGTACA T^GTACA
Bst1107I 174 GTATAC GTA^TAC
BstAPI 2028 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1583 TTCGAA TT^CGAA
BstUI 206 CGCG CG^CG
BstZ17I 174 GTATAC GTA^TAC
CelII 1665 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1903 GCCGGC [GA]^CCGG[CT]
DrdI 1691 GACAAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6245 AGCGCT AGC^GCT
Hin1I 3626 GGCGTC G[GA]^CG[CT]C
HpaI 5856 GTTAAC GTT^AAC
MfeI 4179 CAATTG C^AATTG
MunI 4179 CAATTG C^AATTG
MvnI 206 CGCG CG^CG
NaeI 1903 GCCGGC GCC^GGC
NdeI 5947 CATATG CA^TATG
NgoMIV 1903 GCCGGC G^CCGGC
NheI 3697 GCTAGC G^CTAGC
NspV 1583 TTCGAA TT^CGAA
PaeR7I 1404 CTCGAG C^TCGAG
PflMI 50 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1216 CACGTG CAC^GTG
PmlI 1216 CACGTG CAC^GTG
Psp1406I 623 AACGTT AA^CGTT
PvuI 2173 CGATCG CGAT^CG
RcaI 2039 TCATGA T^CATGA
SfuI 1583 TTCGAA TT^CGAA
SnaBI 4367 TACGTA TAC^GTA
SpeI 1939 ACTAGT A^CTAGT
SphI 2078 GCATGC GCATG^C
SspBI 6289 TGTACA T^GTACA
SspI 3282 AATATT AAT^ATT
Van91I 50 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1404 CTCGAG C^TCGAG
XmnI 5729 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 133 GGTACC G^GTACC
4548 GGTACC
AflII 1812 CTTAAG C^TTAAG
5093 CTTAAG
AhdI 3544 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACCACAAGTC
Asp718I 133 GGTACC G^GTACC
4548 GGTACC
AspEI 3544 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACCACAAGTC
AspI 62 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4378 GACCTGGTC
AvaI 1404 CTCGAG C^[CT]CG[GA]G
5308 CTCGGG
BcnI 402 CCCGG CC^[GC]GG
3246 CCGGG
BfrI 1812 CTTAAG C^TTAAG
5093 CTTAAG
BsiEI 2173 CGATCG CG[GA][CT]^CG
3531 CGGTCG
BsmBI 2606 CGTCTC CGTCTC(1/5)
3628 CGTCTC
BsoBI 1404 CTCGAG C^[CT]CG[GA]G
5308 CTCGGG
BspLU11I 218 ACATGT A^CATGT
6031 ACATGT
DraI 4956 TTTAAA TTT^AAA
5892 TTTAAA
Eam1105I 3544 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4027 GACCACAAGTC
EcoRV 307 GATATC GAT^ATC
337 GATATC
HindIII 899 AAGCTT A^AGCTT
1809 AAGCTT
KpnI 133 GGTACC GGTAC^C
4548 GGTACC
NciI 402 CCCGG CC^[GC]GG
3246 CCGGG
PflFI 62 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4378 GACCTGGTC
ScaI 6115 AGTACT AGT^ACT
6278 AGTACT
SexAI 3767 ACCTGGT A^CC[AT]GGT
4379 ACCTGGT
StuI 4578 AGGCCT AGG^CCT
5253 AGGCCT
Tth111I 62 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4378 GACCTGGTC
XcmI 2883 CCATTCCAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5147 CCATTGCTTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BciVI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII