##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BsaBI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FspI
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NruI NsiI
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1358 GTCTAC GT^[AC][GT]AC
AccIII 200 TCCGGA T^CCGGA
AcsI 1879 AAATTC [GA]^AATT[CT]
AflII 857 CTTAAG C^TTAAG
Alw44I 3551 GTGCAC G^TGCAC
ApaLI 3551 GTGCAC G^TGCAC
ApoI 1879 AAATTC [GA]^AATT[CT]
BciVI 2369 GTATCC GTATCC(6/5)
BclI 4187 TGATCA T^GATCA
BfrI 857 CTTAAG C^TTAAG
BsaI 625 GAGACC GGTCTC(1/5)
BsaWI 200 TCCGGA [AT]^CCGG[AT]
BseAI 200 TCCGGA T^CCGGA
BsiEI 146 CGGCCG CG[GA][CT]^CG
BsmI 3989 GAATGC GAATGC(1/-1)
BspEI 200 TCCGGA T^CCGGA
BspLU11I 4361 ACATGT A^CATGT
BssSI 2762 CACGAG CACGAG(-5/-1)
BstBI 271 TTCGAA TT^CGAA
BstEII 2134 GGTGACC G^GT[ACGT]ACC
BstPI 2134 GGTGACC G^GT[ACGT]ACC
DraIII 601 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 146 CGGCCG C^GGCCG
EclXI 146 CGGCCG C^GGCCG
Eco52I 146 CGGCCG C^GGCCG
EcoO65I 2134 GGTGACC G^GT[ACGT]ACC
FbaI 4187 TGATCA T^GATCA
FseI 3059 GGCCGGCC GGCCGG^CC
KspI 2524 CCGCGG CCGC^GG
MroI 200 TCCGGA T^CCGGA
NotI 145 GCGGCCGC GC^GGCCGC
NspV 271 TTCGAA TT^CGAA
PaeR7I 1041 CTCGAG C^TCGAG
PshAI 2346 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2524 CCGCGG CCGC^GG
SfuI 271 TTCGAA TT^CGAA
XbaI 3115 TCTAGA T^CTAGA
XhoI 1041 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 1193 GTAC GT^AC
3645 GTAC
Aor51HI 30 AGCGCT AGC^GCT
2530 AGCGCT
AvrII 1636 CCTAGG C^CTAGG
3026 CCTAGG
BbsI 274 GAAGAC GAAGAC(2/6)
3131 GAAGAC
BglII 700 AGATCT A^GATCT
1552 AGATCT
BlnI 1636 CCTAGG C^CTAGG
3026 CCTAGG
BpuAI 274 GAAGAC GAAGAC(2/6)
3131 GAAGAC
BsmBI 304 CGTCTC CGTCTC(1/5)
3937 GAGACG
BspMI 4514 ACCTGC ACCTGC(4/8)
3683 GCAGGT
BsrDI 1942 CATTGC GCAATG(2/0)
678 GCAATG
BsrFI 3060 GCCGGC [GA]^CCGG[CT]
3267 GCCGGC
BstAPI 1794 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2152 GCAAATGCTGC
Cfr10I 3060 GCCGGC [GA]^CCGG[CT]
3267 GCCGGC
Eco47III 30 AGCGCT AGC^GCT
2530 AGCGCT
HincII 1226 GTTGAC GT[CT]^[GA]AC
1376 GTCAAC
HindII 1226 GTTGAC GT[CT]^[GA]AC
1376 GTCAAC
MslI 1448 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2449 CATGCGGGTG
NaeI 3060 GCCGGC GCC^GGC
3267 GCCGGC
NgoMIV 3060 GCCGGC G^CCGGC
3267 GCCGGC
RsaI 1193 GTAC GT^AC
3645 GTAC
SapI 830 GAAGAGC GCTCTTC(1/4)
3669 GCTCTTC
SexAI 461 ACCAGGT A^CC[AT]GGT
3298 ACCAGGT
SfiI 147 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
718 GGCCGCCCTGGCC
SmaI 2247 CCCGGG CCC^GGG
2277 CCCGGG
SphI 431 GCATGC GCATG^C
919 GCATGC
XmaCI 2247 CCCGGG C^CCGGG
2277 CCCGGG
XmaI 2247 CCCGGG C^CCGGG
2277 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII