################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI AspEI AvrII BbeI BclI BfrI BglI BglII BlnI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I RsrII SacII SalI SfiI SfoI SnaBI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 594 GGTACC G^GTACC AccII 2454 CGCG CG^CG Alw44I 1143 GTGCAC G^TGCAC ApaLI 1143 GTGCAC G^TGCAC Asp718I 594 GGTACC G^GTACC AviII 2323 TGCGCA TGC^GCA BamHI 1176 GGATCC G^GATCC BbrPI 4883 CACGTG CAC^GTG BcgI 239 GCAGAGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4084 GTATCC GTATCC(6/5) BsaAI 4883 CACGTG [CT]AC^GT[GA] BsaWI 4508 TCCGGT [AT]^CCGG[AT] BsiEI 1837 CGATCG CG[GA][CT]^CG BspLU11I 4417 ACATGT A^CATGT BstBI 2999 TTCGAA TT^CGAA BstEII 1579 GGTCACC G^GT[ACGT]ACC BstPI 1579 GGTCACC G^GT[ACGT]ACC BstUI 2454 CGCG CG^CG DrdI 129 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1579 GGTCACC G^GT[ACGT]ACC FspI 2323 TGCGCA TGC^GCA HincII 474 GTCAAC GT[CT]^[GA]AC HindII 474 GTCAAC GT[CT]^[GA]AC KpnI 594 GGTACC GGTAC^C MvnI 2454 CGCG CG^CG NaeI 714 GCCGGC GCC^GGC NgoMIV 714 GCCGGC G^CCGGC NspV 2999 TTCGAA TT^CGAA PmaCI 4883 CACGTG CAC^GTG PmlI 4883 CACGTG CAC^GTG PshAI 1242 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1837 CGATCG CGAT^CG SacI 4773 GAGCTC GAGCT^C SbfI 2351 CCTGCAGG CCTGCA^GG ScaI 6015 AGTACT AGT^ACT SfuI 2999 TTCGAA TT^CGAA SgrAI 628 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 2351 CCTGCAGG CCTGCA^GG SspI 5974 AATATT AAT^ATT XbaI 5843 TCTAGA T^CTAGA XcmI 2500 CCACTCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 4417 ACATGT A^C[GA][CT]GT 5157 ACGTGT Asp700I 1700 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5953 GAATTTTTTC AspI 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5713 GACTCGGTC BsmBI 249 CGTCTC CGTCTC(1/5) 1324 GAGACG BspMI 3880 ACCTGC ACCTGC(4/8) 3931 GCAGGT BsrFI 629 GCCGGT [GA]^CCGG[CT] 714 GCCGGC BstAPI 1145 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4909 GCACTTCCTGC Cfr10I 629 GCCGGT [GA]^CCGG[CT] 714 GCCGGC EcoNI 728 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5024 CCTTGGCCAGG PflFI 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5713 GACTCGGTC SapI 883 GAAGAGC GCTCTTC(1/4) 1871 GCTCTTC SexAI 614 ACCAGGT A^CC[AT]GGT 5598 ACCAGGT SmaI 4520 CCCGGG CCC^GGG 5232 CCCGGG SphI 2299 GCATGC GCATG^C 2326 GCATGC StuI 204 AGGCCT AGG^CCT 2635 AGGCCT Tth111I 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5713 GACTCGGTC XmaCI 4520 CCCGGG C^CCGGG 5232 CCCGGG XmaI 4520 CCCGGG C^CCGGG 5232 CCCGGG XmnI 1700 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5953 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII