##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
AspEI AvrII BbeI BclI BfrI
BglI BglII BlnI BsaBI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I RsrII SacII
SalI SfiI SfoI SnaBI SpeI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 594 GGTACC G^GTACC
AccII 2454 CGCG CG^CG
Alw44I 1143 GTGCAC G^TGCAC
ApaLI 1143 GTGCAC G^TGCAC
Asp718I 594 GGTACC G^GTACC
AviII 2323 TGCGCA TGC^GCA
BamHI 1176 GGATCC G^GATCC
BbrPI 4883 CACGTG CAC^GTG
BcgI 239 GCAGAGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4084 GTATCC GTATCC(6/5)
BsaAI 4883 CACGTG [CT]AC^GT[GA]
BsaWI 4508 TCCGGT [AT]^CCGG[AT]
BsiEI 1837 CGATCG CG[GA][CT]^CG
BspLU11I 4417 ACATGT A^CATGT
BstBI 2999 TTCGAA TT^CGAA
BstEII 1579 GGTCACC G^GT[ACGT]ACC
BstPI 1579 GGTCACC G^GT[ACGT]ACC
BstUI 2454 CGCG CG^CG
DrdI 129 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1579 GGTCACC G^GT[ACGT]ACC
FspI 2323 TGCGCA TGC^GCA
HincII 474 GTCAAC GT[CT]^[GA]AC
HindII 474 GTCAAC GT[CT]^[GA]AC
KpnI 594 GGTACC GGTAC^C
MvnI 2454 CGCG CG^CG
NaeI 714 GCCGGC GCC^GGC
NgoMIV 714 GCCGGC G^CCGGC
NspV 2999 TTCGAA TT^CGAA
PmaCI 4883 CACGTG CAC^GTG
PmlI 4883 CACGTG CAC^GTG
PshAI 1242 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1837 CGATCG CGAT^CG
SacI 4773 GAGCTC GAGCT^C
SbfI 2351 CCTGCAGG CCTGCA^GG
ScaI 6015 AGTACT AGT^ACT
SfuI 2999 TTCGAA TT^CGAA
SgrAI 628 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 2351 CCTGCAGG CCTGCA^GG
SspI 5974 AATATT AAT^ATT
XbaI 5843 TCTAGA T^CTAGA
XcmI 2500 CCACTCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 4417 ACATGT A^C[GA][CT]GT
5157 ACGTGT
Asp700I 1700 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5953 GAATTTTTTC
AspI 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5713 GACTCGGTC
BsmBI 249 CGTCTC CGTCTC(1/5)
1324 GAGACG
BspMI 3880 ACCTGC ACCTGC(4/8)
3931 GCAGGT
BsrFI 629 GCCGGT [GA]^CCGG[CT]
714 GCCGGC
BstAPI 1145 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4909 GCACTTCCTGC
Cfr10I 629 GCCGGT [GA]^CCGG[CT]
714 GCCGGC
EcoNI 728 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5024 CCTTGGCCAGG
PflFI 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5713 GACTCGGTC
SapI 883 GAAGAGC GCTCTTC(1/4)
1871 GCTCTTC
SexAI 614 ACCAGGT A^CC[AT]GGT
5598 ACCAGGT
SmaI 4520 CCCGGG CCC^GGG
5232 CCCGGG
SphI 2299 GCATGC GCATG^C
2326 GCATGC
StuI 204 AGGCCT AGG^CCT
2635 AGGCCT
Tth111I 5277 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5713 GACTCGGTC
XmaCI 4520 CCCGGG C^CCGGG
5232 CCCGGG
XmaI 4520 CCCGGG C^CCGGG
5232 CCCGGG
XmnI 1700 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5953 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BsrBI BsrDI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII