################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccIII AclI AgeI ApaI AscI AviII BbeI BcgI BcnI BglII BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I EcoO65I FseI FspI HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NciI NgoMIV NotI NruI PacI PaeR7I PflMI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1753 GGTACC G^GTACC AcyI 1502 GGCGTC G[GA]^CG[CT]C AhdI 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2176 GTGCAC G^TGCAC Aor51HI 1655 AGCGCT AGC^GCT ApaLI 2176 GTGCAC G^TGCAC Asp700I 3275 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1753 GGTACC G^GTACC AspEI 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3167 CCCGAG C^[CT]CG[GA]G AvrII 4363 CCTAGG C^CTAGG BalI 1282 TGGCCA TGG^CCA BamHI 3367 GGATCC G^GATCC BbrPI 2932 CACGTG CAC^GTG BlnI 4363 CCTAGG C^CTAGG BsaAI 2932 CACGTG [CT]AC^GT[GA] BsaHI 1502 GGCGTC G[GA]^CG[CT]C BsoBI 3167 CCCGAG C^[CT]CG[GA]G BsrBI 3346 GAGCGG CCGCTC(-3/-3) BssSI 2288 CTCGTG CACGAG(-5/-1) BstBI 1637 TTCGAA TT^CGAA DraIII 3813 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4905 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1655 AGCGCT AGC^GCT EcoNI 4972 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3118 GATATC GAT^ATC EcoT22I 4844 ATGCAT ATGCA^T FauI 3729 CCCGC CCCGC(4/6) HapII 3422 CCGG C^CGG Hin1I 1502 GGCGTC G[GA]^CG[CT]C HpaII 3422 CCGG C^CGG KpnI 1753 GGTACC GGTAC^C MluNI 1282 TGGCCA TGG^CCA MscI 1282 TGGCCA TGG^CCA MspI 3422 CCGG C^CGG NdeI 4812 CATATG CA^TATG NheI 1577 GCTAGC G^CTAGC NsiI 4844 ATGCAT ATGCA^T NspV 1637 TTCGAA TT^CGAA PflFI 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2932 CACGTG CAC^GTG PmlI 2932 CACGTG CAC^GTG SalI 3097 GTCGAC G^TCGAC ScaI 2681 AGTACT AGT^ACT SfuI 1637 TTCGAA TT^CGAA Tth111I 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3275 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 3097 GTCGAC GT^[AC][GT]AC 4214 GTAGAC AccII 2057 CGCG CG^CG 4902 CGCG AflII 5237 CTTAAG C^TTAAG 5264 CTTAAG AocI 1973 CCTCAGG CC^T[ACGT]AGG 3029 CCTGAGG AseI 4721 ATTAAT AT^TAAT 4767 ATTAAT AsnI 4721 ATTAAT AT^TAAT 4767 ATTAAT BciVI 357 GGATAC GTATCC(6/5) 2758 GTATCC BclI 3691 TGATCA T^GATCA 5295 TGATCA BfrI 5237 CTTAAG C^TTAAG 5264 CTTAAG BglI 2418 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4931 GCCCCTGTGGC BsaBI 3689 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4639 GATGCTAATC Bsp1407I 230 TGTACA T^GTACA 4431 TGTACA BsrGI 230 TGTACA T^GTACA 4431 TGTACA BstAPI 689 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2463 GCATGGACTGC BstUI 2057 CGCG CG^CG 4902 CGCG Bsu36I 1973 CCTCAGG CC^T[ACGT]AGG 3029 CCTGAGG EaeI 1282 TGGCCA [CT]^GGCC[GA] 1335 CGGCCA Eco81I 1973 CCTCAGG CC^T[ACGT]AGG 3029 CCTGAGG EcoRI 1634 GAATTC G^AATTC 3557 GAATTC FbaI 3691 TGATCA T^GATCA 5295 TGATCA HaeII 1655 AGCGCT [GA]GCGC^[CT] 3999 AGCGCC HincII 2170 GTTGAC GT[CT]^[GA]AC 3097 GTCGAC HindII 2170 GTTGAC GT[CT]^[GA]AC 3097 GTCGAC MamI 3689 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4639 GATGCTAATC MspA1I 2859 CAGCTG C[AC]G^C[GT]G 3201 CAGCTG MvnI 2057 CGCG CG^CG 4902 CGCG PshBI 4721 ATTAAT AT^TAAT 4767 ATTAAT PstI 3741 CTGCAG CTGCA^G 5000 CTGCAG PvuII 2859 CAGCTG CAG^CTG 3201 CAGCTG SacI 2515 GAGCTC GAGCT^C 4188 GAGCTC SmlI 5237 CTTAAG C^T[CT][GA]AG 5264 CTTAAG SpeI 1409 ACTAGT A^CTAGT 2939 ACTAGT SphI 2727 GCATGC GCATG^C 4842 GCATGC SspBI 230 TGTACA T^GTACA 4431 TGTACA XcmI 1059 CCAAAGGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4568 CCAGGCTGCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII