##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AviII BbeI BcgI BcnI
BglII BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrFI BssHII Bst1107I BstEII BstPI
BstZ17I Cfr10I ClaI CpoI EagI
EclXI Eco52I EcoO65I FseI FspI
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NciI
NgoMIV NotI NruI PacI PaeR7I
PflMI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1753 GGTACC G^GTACC
AcyI 1502 GGCGTC G[GA]^CG[CT]C
AhdI 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2176 GTGCAC G^TGCAC
Aor51HI 1655 AGCGCT AGC^GCT
ApaLI 2176 GTGCAC G^TGCAC
Asp700I 3275 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1753 GGTACC G^GTACC
AspEI 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3167 CCCGAG C^[CT]CG[GA]G
AvrII 4363 CCTAGG C^CTAGG
BalI 1282 TGGCCA TGG^CCA
BamHI 3367 GGATCC G^GATCC
BbrPI 2932 CACGTG CAC^GTG
BlnI 4363 CCTAGG C^CTAGG
BsaAI 2932 CACGTG [CT]AC^GT[GA]
BsaHI 1502 GGCGTC G[GA]^CG[CT]C
BsoBI 3167 CCCGAG C^[CT]CG[GA]G
BsrBI 3346 GAGCGG CCGCTC(-3/-3)
BssSI 2288 CTCGTG CACGAG(-5/-1)
BstBI 1637 TTCGAA TT^CGAA
DraIII 3813 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4905 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2629 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1655 AGCGCT AGC^GCT
EcoNI 4972 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3118 GATATC GAT^ATC
EcoT22I 4844 ATGCAT ATGCA^T
FauI 3729 CCCGC CCCGC(4/6)
HapII 3422 CCGG C^CGG
Hin1I 1502 GGCGTC G[GA]^CG[CT]C
HpaII 3422 CCGG C^CGG
KpnI 1753 GGTACC GGTAC^C
MluNI 1282 TGGCCA TGG^CCA
MscI 1282 TGGCCA TGG^CCA
MspI 3422 CCGG C^CGG
NdeI 4812 CATATG CA^TATG
NheI 1577 GCTAGC G^CTAGC
NsiI 4844 ATGCAT ATGCA^T
NspV 1637 TTCGAA TT^CGAA
PflFI 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2932 CACGTG CAC^GTG
PmlI 2932 CACGTG CAC^GTG
SalI 3097 GTCGAC G^TCGAC
ScaI 2681 AGTACT AGT^ACT
SfuI 1637 TTCGAA TT^CGAA
Tth111I 1499 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3275 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3097 GTCGAC GT^[AC][GT]AC
4214 GTAGAC
AccII 2057 CGCG CG^CG
4902 CGCG
AflII 5237 CTTAAG C^TTAAG
5264 CTTAAG
AocI 1973 CCTCAGG CC^T[ACGT]AGG
3029 CCTGAGG
AseI 4721 ATTAAT AT^TAAT
4767 ATTAAT
AsnI 4721 ATTAAT AT^TAAT
4767 ATTAAT
BciVI 357 GGATAC GTATCC(6/5)
2758 GTATCC
BclI 3691 TGATCA T^GATCA
5295 TGATCA
BfrI 5237 CTTAAG C^TTAAG
5264 CTTAAG
BglI 2418 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4931 GCCCCTGTGGC
BsaBI 3689 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4639 GATGCTAATC
Bsp1407I 230 TGTACA T^GTACA
4431 TGTACA
BsrGI 230 TGTACA T^GTACA
4431 TGTACA
BstAPI 689 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2463 GCATGGACTGC
BstUI 2057 CGCG CG^CG
4902 CGCG
Bsu36I 1973 CCTCAGG CC^T[ACGT]AGG
3029 CCTGAGG
EaeI 1282 TGGCCA [CT]^GGCC[GA]
1335 CGGCCA
Eco81I 1973 CCTCAGG CC^T[ACGT]AGG
3029 CCTGAGG
EcoRI 1634 GAATTC G^AATTC
3557 GAATTC
FbaI 3691 TGATCA T^GATCA
5295 TGATCA
HaeII 1655 AGCGCT [GA]GCGC^[CT]
3999 AGCGCC
HincII 2170 GTTGAC GT[CT]^[GA]AC
3097 GTCGAC
HindII 2170 GTTGAC GT[CT]^[GA]AC
3097 GTCGAC
MamI 3689 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4639 GATGCTAATC
MspA1I 2859 CAGCTG C[AC]G^C[GT]G
3201 CAGCTG
MvnI 2057 CGCG CG^CG
4902 CGCG
PshBI 4721 ATTAAT AT^TAAT
4767 ATTAAT
PstI 3741 CTGCAG CTGCA^G
5000 CTGCAG
PvuII 2859 CAGCTG CAG^CTG
3201 CAGCTG
SacI 2515 GAGCTC GAGCT^C
4188 GAGCTC
SmlI 5237 CTTAAG C^T[CT][GA]AG
5264 CTTAAG
SpeI 1409 ACTAGT A^CTAGT
2939 ACTAGT
SphI 2727 GCATGC GCATG^C
4842 GCATGC
SspBI 230 TGTACA T^GTACA
4431 TGTACA
XcmI 1059 CCAAAGGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4568 CCAGGCTGCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII