################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccII AccIII AclI AcyI AocI ApaI AscI AspI AviII AvrII BbeI BbrPI BcnI BglI BlnI BsaHI BseAI BsiEI BsiWI BspEI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CpoI DrdI EagI EclXI Eco52I Eco81I FseI FspI Hin1I KasI KspI MluI MroI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 110 GGTACC G^GTACC AflII 1548 CTTAAG C^TTAAG AgeI 5423 ACCGGT A^CCGGT AhdI 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3763 GTGCAC G^TGCAC ApaLI 3763 GTGCAC G^TGCAC Asp718I 110 GGTACC G^GTACC AspEI 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1031 TGGCCA TGG^CCA BamHI 3953 GGATCC G^GATCC BcgI 3926 GCAAGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3192 GGATAC GTATCC(6/5) BclI 2828 TGATCA T^GATCA BfrI 1548 CTTAAG C^TTAAG BlpI 2478 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2478 GCTGAGC GC^T[ACGT]AGC BsaAI 773 CACGTA [CT]AC^GT[GA] BsaWI 5423 ACCGGT [AT]^CCGG[AT] BsmI 3140 GCATTC GAATGC(1/-1) BspDI 3453 ATCGAT AT^CGAT BspHI 3632 TCATGA T^CATGA BspMI 2840 GCAGGT ACCTGC(4/8) BsrFI 5423 ACCGGT [GA]^CCGG[CT] BssSI 3439 CACGAG CACGAG(-5/-1) BstAPI 5293 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3336 GGTGACC G^GT[ACGT]ACC BstPI 3336 GGTGACC G^GT[ACGT]ACC BstXI 3008 CCAGTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2478 GCTGAGC GC^T[ACGT]AGC Cfr10I 5423 ACCGGT [GA]^CCGG[CT] ClaI 3453 ATCGAT AT^CGAT DraII 5314 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 3038 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1031 TGGCCA [CT]^GGCC[GA] Eam1105I 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3900 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 5314 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 3336 GGTGACC G^GT[ACGT]ACC EcoRV 1522 GATATC GAT^ATC EcoT14I 5204 CCTTGG C^C[AT][AT]GG FbaI 2828 TGATCA T^GATCA HgaI 1151 GACGC GACGC(5/10) HindIII 1012 AAGCTT A^AGCTT HpaI 1833 GTTAAC GTT^AAC KpnI 110 GGTACC GGTAC^C MluNI 1031 TGGCCA TGG^CCA MscI 1031 TGGCCA TGG^CCA PinAI 5423 ACCGGT A^CCGGT PpuMI 5314 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 2085 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3632 TCATGA T^CATGA SbfI 2837 CCTGCAGG CCTGCA^GG Sse8387I 2837 CCTGCAGG CCTGCA^GG StyI 5204 CCTTGG C^C[AT][AT]GG SwaI 5871 ATTTAAAT ATTT^AAAT XcmI 14 CCATAAGAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 124 GTCTAC GT^[AC][GT]AC 2969 GTCTAC Aor51HI 5060 AGCGCT AGC^GCT 5434 AGCGCT Asp700I 180 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 252 GAAGAGCTTC AvaI 4560 CCCGAG C^[CT]CG[GA]G 5319 CCCGAG BsmBI 2560 CGTCTC CGTCTC(1/5) 1149 GAGACG BsoBI 4560 CCCGAG C^[CT]CG[GA]G 5319 CCCGAG BspLU11I 828 ACATGT A^CATGT 1351 ACATGT BsrDI 168 CATTGC GCAATG(2/0) 338 CATTGC DsaI 1368 CCACGG C^C[GA][CT]GG 2131 CCGTGG Eco47III 5060 AGCGCT AGC^GCT 5434 AGCGCT EcoT22I 4972 ATGCAT ATGCA^T 5582 ATGCAT FauI 1445 GCGGG CCCGC(4/6) 3641 GCGGG HapII 882 CCGG C^CGG 5424 CCGG HpaII 882 CCGG C^CGG 5424 CCGG MspI 882 CCGG C^CGG 5424 CCGG NsiI 4972 ATGCAT ATGCA^T 5582 ATGCAT SacI 225 GAGCTC GAGCT^C 4867 GAGCTC SmlI 1450 CTCAAG C^T[CT][GA]AG 1548 CTTAAG SpeI 3263 ACTAGT A^CTAGT 3552 ACTAGT StuI 3474 AGGCCT AGG^CCT 5323 AGGCCT XmnI 180 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 252 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanI BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII