##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AocI ApaI AscI AspI AviII
AvrII BbeI BbrPI BcnI BglI
BlnI BsaHI BseAI BsiEI BsiWI
BspEI BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I CpoI DrdI
EagI EclXI Eco52I Eco81I FseI
FspI Hin1I KasI KspI MluI
MroI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 110 GGTACC G^GTACC
AflII 1548 CTTAAG C^TTAAG
AgeI 5423 ACCGGT A^CCGGT
AhdI 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3763 GTGCAC G^TGCAC
ApaLI 3763 GTGCAC G^TGCAC
Asp718I 110 GGTACC G^GTACC
AspEI 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1031 TGGCCA TGG^CCA
BamHI 3953 GGATCC G^GATCC
BcgI 3926 GCAAGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3192 GGATAC GTATCC(6/5)
BclI 2828 TGATCA T^GATCA
BfrI 1548 CTTAAG C^TTAAG
BlpI 2478 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2478 GCTGAGC GC^T[ACGT]AGC
BsaAI 773 CACGTA [CT]AC^GT[GA]
BsaWI 5423 ACCGGT [AT]^CCGG[AT]
BsmI 3140 GCATTC GAATGC(1/-1)
BspDI 3453 ATCGAT AT^CGAT
BspHI 3632 TCATGA T^CATGA
BspMI 2840 GCAGGT ACCTGC(4/8)
BsrFI 5423 ACCGGT [GA]^CCGG[CT]
BssSI 3439 CACGAG CACGAG(-5/-1)
BstAPI 5293 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3336 GGTGACC G^GT[ACGT]ACC
BstPI 3336 GGTGACC G^GT[ACGT]ACC
BstXI 3008 CCAGTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2478 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5423 ACCGGT [GA]^CCGG[CT]
ClaI 3453 ATCGAT AT^CGAT
DraII 5314 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 3038 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1031 TGGCCA [CT]^GGCC[GA]
Eam1105I 3695 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3900 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 5314 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3336 GGTGACC G^GT[ACGT]ACC
EcoRV 1522 GATATC GAT^ATC
EcoT14I 5204 CCTTGG C^C[AT][AT]GG
FbaI 2828 TGATCA T^GATCA
HgaI 1151 GACGC GACGC(5/10)
HindIII 1012 AAGCTT A^AGCTT
HpaI 1833 GTTAAC GTT^AAC
KpnI 110 GGTACC GGTAC^C
MluNI 1031 TGGCCA TGG^CCA
MscI 1031 TGGCCA TGG^CCA
PinAI 5423 ACCGGT A^CCGGT
PpuMI 5314 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2085 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3632 TCATGA T^CATGA
SbfI 2837 CCTGCAGG CCTGCA^GG
Sse8387I 2837 CCTGCAGG CCTGCA^GG
StyI 5204 CCTTGG C^C[AT][AT]GG
SwaI 5871 ATTTAAAT ATTT^AAAT
XcmI 14 CCATAAGAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 124 GTCTAC GT^[AC][GT]AC
2969 GTCTAC
Aor51HI 5060 AGCGCT AGC^GCT
5434 AGCGCT
Asp700I 180 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
252 GAAGAGCTTC
AvaI 4560 CCCGAG C^[CT]CG[GA]G
5319 CCCGAG
BsmBI 2560 CGTCTC CGTCTC(1/5)
1149 GAGACG
BsoBI 4560 CCCGAG C^[CT]CG[GA]G
5319 CCCGAG
BspLU11I 828 ACATGT A^CATGT
1351 ACATGT
BsrDI 168 CATTGC GCAATG(2/0)
338 CATTGC
DsaI 1368 CCACGG C^C[GA][CT]GG
2131 CCGTGG
Eco47III 5060 AGCGCT AGC^GCT
5434 AGCGCT
EcoT22I 4972 ATGCAT ATGCA^T
5582 ATGCAT
FauI 1445 GCGGG CCCGC(4/6)
3641 GCGGG
HapII 882 CCGG C^CGG
5424 CCGG
HpaII 882 CCGG C^CGG
5424 CCGG
MspI 882 CCGG C^CGG
5424 CCGG
NsiI 4972 ATGCAT ATGCA^T
5582 ATGCAT
SacI 225 GAGCTC GAGCT^C
4867 GAGCTC
SmlI 1450 CTCAAG C^T[CT][GA]AG
1548 CTTAAG
SpeI 3263 ACTAGT A^CTAGT
3552 ACTAGT
StuI 3474 AGGCCT AGG^CCT
5323 AGGCCT
XmnI 180 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
252 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII