##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AocI        ApaI        AscI        AspI        AviII       
AvrII       BbeI        BbrPI       BcnI        BglI        
BlnI        BsaHI       BseAI       BsiEI       BsiWI       
BspEI       BsrBI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      CpoI        DrdI        
EagI        EclXI       Eco52I      Eco81I      FseI        
FspI        Hin1I       KasI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        110  GGTACC               G^GTACC
AflII        1548  CTTAAG               C^TTAAG
AgeI         5423  ACCGGT               A^CCGGT
AhdI         3695  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3763  GTGCAC               G^TGCAC
ApaLI        3763  GTGCAC               G^TGCAC
Asp718I       110  GGTACC               G^GTACC
AspEI        3695  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1031  TGGCCA               TGG^CCA
BamHI        3953  GGATCC               G^GATCC
BcgI         3926  GCAAGAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3192  GGATAC               GTATCC(6/5)
BclI         2828  TGATCA               T^GATCA
BfrI         1548  CTTAAG               C^TTAAG
BlpI         2478  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2478  GCTGAGC              GC^T[ACGT]AGC
BsaAI         773  CACGTA               [CT]AC^GT[GA]
BsaWI        5423  ACCGGT               [AT]^CCGG[AT]
BsmI         3140  GCATTC               GAATGC(1/-1)
BspDI        3453  ATCGAT               AT^CGAT
BspHI        3632  TCATGA               T^CATGA
BspMI        2840  GCAGGT               ACCTGC(4/8)
BsrFI        5423  ACCGGT               [GA]^CCGG[CT]
BssSI        3439  CACGAG               CACGAG(-5/-1)
BstAPI       5293  GCAGAGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3336  GGTGACC              G^GT[ACGT]ACC
BstPI        3336  GGTGACC              G^GT[ACGT]ACC
BstXI        3008  CCAGTACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2478  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       5423  ACCGGT               [GA]^CCGG[CT]
ClaI         3453  ATCGAT               AT^CGAT
DraII        5314  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       3038  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1031  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3695  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3900  CCTAGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     5314  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      3336  GGTGACC              G^GT[ACGT]ACC
EcoRV        1522  GATATC               GAT^ATC
EcoT14I      5204  CCTTGG               C^C[AT][AT]GG
FbaI         2828  TGATCA               T^GATCA
HgaI         1151  GACGC                GACGC(5/10)
HindIII      1012  AAGCTT               A^AGCTT
HpaI         1833  GTTAAC               GTT^AAC
KpnI          110  GGTACC               GGTAC^C
MluNI        1031  TGGCCA               TGG^CCA
MscI         1031  TGGCCA               TGG^CCA
PinAI        5423  ACCGGT               A^CCGGT
PpuMI        5314  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        2085  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3632  TCATGA               T^CATGA
SbfI         2837  CCTGCAGG             CCTGCA^GG
Sse8387I     2837  CCTGCAGG             CCTGCA^GG
StyI         5204  CCTTGG               C^C[AT][AT]GG
SwaI         5871  ATTTAAAT             ATTT^AAAT
XcmI           14  CCATAAGAATGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          124  GTCTAC               GT^[AC][GT]AC
             2969  GTCTAC              
Aor51HI      5060  AGCGCT               AGC^GCT
             5434  AGCGCT              
Asp700I       180  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              252  GAAGAGCTTC          
AvaI         4560  CCCGAG               C^[CT]CG[GA]G
             5319  CCCGAG              
BsmBI        2560  CGTCTC               CGTCTC(1/5)
             1149  GAGACG              
BsoBI        4560  CCCGAG               C^[CT]CG[GA]G
             5319  CCCGAG              
BspLU11I      828  ACATGT               A^CATGT
             1351  ACATGT              
BsrDI         168  CATTGC               GCAATG(2/0)
              338  CATTGC              
DsaI         1368  CCACGG               C^C[GA][CT]GG
             2131  CCGTGG              
Eco47III     5060  AGCGCT               AGC^GCT
             5434  AGCGCT              
EcoT22I      4972  ATGCAT               ATGCA^T
             5582  ATGCAT              
FauI         1445  GCGGG                CCCGC(4/6)
             3641  GCGGG               
HapII         882  CCGG                 C^CGG
             5424  CCGG                
HpaII         882  CCGG                 C^CGG
             5424  CCGG                
MspI          882  CCGG                 C^CGG
             5424  CCGG                
NsiI         4972  ATGCAT               ATGCA^T
             5582  ATGCAT              
SacI          225  GAGCTC               GAGCT^C
             4867  GAGCTC              
SmlI         1450  CTCAAG               C^T[CT][GA]AG
             1548  CTTAAG              
SpeI         3263  ACTAGT               A^CTAGT
             3552  ACTAGT              
StuI         3474  AGGCCT               AGG^CCT
             5323  AGGCCT              
XmnI          180  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              252  GAAGAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
AspHI       AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaBI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRI       EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HhaI        HinP1I      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MfeI        MflI        MnlI        
MseI        MslI        MspA1I      MunI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PshBI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SspBI       SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XhoII