##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I AspEI BbeI
BbvCI BsiWI BsmBI BsmI BspDI
BssHII BstBI BstUI ClaI CpoI
Eam1105I Eco47III EcoRV EcoT22I FseI
HpaI KasI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 490 GACGTC GACGT^C
AccI 232 GTATAC GT^[AC][GT]AC
AccIII 554 TCCGGA T^CCGGA
AflII 5386 CTTAAG C^TTAAG
Alw44I 457 GTGCAC G^TGCAC
ApaLI 457 GTGCAC G^TGCAC
AviII 4566 TGCGCA TGC^GCA
AvrII 4048 CCTAGG C^CTAGG
BamHI 872 GGATCC G^GATCC
BbrPI 460 CACGTG CAC^GTG
BfrI 5386 CTTAAG C^TTAAG
BlnI 4048 CCTAGG C^CTAGG
BseAI 554 TCCGGA T^CCGGA
BspEI 554 TCCGGA T^CCGGA
BsrBI 1194 CCGCTC CCGCTC(-3/-3)
Bst1107I 232 GTATAC GTA^TAC
BstZ17I 232 GTATAC GTA^TAC
DraIII 2734 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4071 GACACGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1305 CGGCCG C^GGCCG
EclXI 1305 CGGCCG C^GGCCG
Eco52I 1305 CGGCCG C^GGCCG
EcoNI 3157 CCTTCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 4566 TGCGCA TGC^GCA
MroI 554 TCCGGA T^CCGGA
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
SapI 5554 GCTCTTC GCTCTTC(1/4)
SexAI 713 ACCTGGT A^CC[AT]GGT
SphI 1565 GCATGC GCATG^C
XbaI 3096 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 363 AACGTT AA^CGTT
4582 AACGTT
ApaI 1468 GGGCCC GGGCC^C
3395 GGGCCC
AvaI 2845 CTCGGG C^[CT]CG[GA]G
3398 CCCGAG
BcgI 2609 GCAACAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5432 GCAGGAGAATCG
BciVI 3991 GGATAC GTATCC(6/5)
2185 GTATCC
BglI 201 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1917 GCCACTCTGGC
BlpI 1962 GCTGAGC GC^T[ACGT]AGC
2334 GCTGAGC
Bpu1102I 1962 GCTGAGC GC^T[ACGT]AGC
2334 GCTGAGC
BsaAI 460 CACGTG [CT]AC^GT[GA]
1065 CACGTA
BsaBI 847 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2478 GATCATCATC
BsaWI 554 TCCGGA [AT]^CCGG[AT]
1763 TCCGGT
BsiEI 1305 CGGCCG CG[GA][CT]^CG
3518 CGGTCG
BsoBI 2845 CTCGGG C^[CT]CG[GA]G
3398 CCCGAG
BspLU11I 8 ACATGT A^CATGT
2636 ACATGT
BsrDI 963 CATTGC GCAATG(2/0)
344 GCAATG
BsrFI 1303 ACCGGC [GA]^CCGG[CT]
5080 ACCGGC
CelII 1962 GCTGAGC GC^T[ACGT]AGC
2334 GCTGAGC
Cfr10I 1303 ACCGGC [GA]^CCGG[CT]
5080 ACCGGC
DraI 3809 TTTAAA TTT^AAA
4894 TTTAAA
EcoRI 4847 GAATTC G^AATTC
5306 GAATTC
HindIII 1377 AAGCTT A^AGCTT
2521 AAGCTT
MamI 847 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2478 GATCATCATC
NheI 294 GCTAGC G^CTAGC
5003 GCTAGC
Psp1406I 363 AACGTT AA^CGTT
4582 AACGTT
StuI 3610 AGGCCT AGG^CCT
4822 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp718I AspHI AspI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspMI BsrGI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII