################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AccII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI BbeI BbvCI BsiWI BsmBI BsmI BspDI BssHII BstBI BstUI ClaI CpoI Eam1105I Eco47III EcoRV EcoT22I FseI HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 490 GACGTC GACGT^C AccI 232 GTATAC GT^[AC][GT]AC AccIII 554 TCCGGA T^CCGGA AflII 5386 CTTAAG C^TTAAG Alw44I 457 GTGCAC G^TGCAC ApaLI 457 GTGCAC G^TGCAC AviII 4566 TGCGCA TGC^GCA AvrII 4048 CCTAGG C^CTAGG BamHI 872 GGATCC G^GATCC BbrPI 460 CACGTG CAC^GTG BfrI 5386 CTTAAG C^TTAAG BlnI 4048 CCTAGG C^CTAGG BseAI 554 TCCGGA T^CCGGA BspEI 554 TCCGGA T^CCGGA BsrBI 1194 CCGCTC CCGCTC(-3/-3) Bst1107I 232 GTATAC GTA^TAC BstZ17I 232 GTATAC GTA^TAC DraIII 2734 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4071 GACACGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1305 CGGCCG C^GGCCG EclXI 1305 CGGCCG C^GGCCG Eco52I 1305 CGGCCG C^GGCCG EcoNI 3157 CCTTCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 4566 TGCGCA TGC^GCA MroI 554 TCCGGA T^CCGGA PmaCI 460 CACGTG CAC^GTG PmlI 460 CACGTG CAC^GTG SapI 5554 GCTCTTC GCTCTTC(1/4) SexAI 713 ACCTGGT A^CC[AT]GGT SphI 1565 GCATGC GCATG^C XbaI 3096 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AclI 363 AACGTT AA^CGTT 4582 AACGTT ApaI 1468 GGGCCC GGGCC^C 3395 GGGCCC AvaI 2845 CTCGGG C^[CT]CG[GA]G 3398 CCCGAG BcgI 2609 GCAACAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5432 GCAGGAGAATCG BciVI 3991 GGATAC GTATCC(6/5) 2185 GTATCC BglI 201 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1917 GCCACTCTGGC BlpI 1962 GCTGAGC GC^T[ACGT]AGC 2334 GCTGAGC Bpu1102I 1962 GCTGAGC GC^T[ACGT]AGC 2334 GCTGAGC BsaAI 460 CACGTG [CT]AC^GT[GA] 1065 CACGTA BsaBI 847 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2478 GATCATCATC BsaWI 554 TCCGGA [AT]^CCGG[AT] 1763 TCCGGT BsiEI 1305 CGGCCG CG[GA][CT]^CG 3518 CGGTCG BsoBI 2845 CTCGGG C^[CT]CG[GA]G 3398 CCCGAG BspLU11I 8 ACATGT A^CATGT 2636 ACATGT BsrDI 963 CATTGC GCAATG(2/0) 344 GCAATG BsrFI 1303 ACCGGC [GA]^CCGG[CT] 5080 ACCGGC CelII 1962 GCTGAGC GC^T[ACGT]AGC 2334 GCTGAGC Cfr10I 1303 ACCGGC [GA]^CCGG[CT] 5080 ACCGGC DraI 3809 TTTAAA TTT^AAA 4894 TTTAAA EcoRI 4847 GAATTC G^AATTC 5306 GAATTC HindIII 1377 AAGCTT A^AGCTT 2521 AAGCTT MamI 847 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2478 GATCATCATC NheI 294 GCTAGC G^CTAGC 5003 GCTAGC Psp1406I 363 AACGTT AA^CGTT 4582 AACGTT StuI 3610 AGGCCT AGG^CCT 4822 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp718I AspHI AspI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII