##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AccIII AclI AflII
Alw44I ApaLI AscI AspI BbrPI
BciVI BfrI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI HindIII HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SspBI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3095 GGTACC G^GTACC
AgeI 1687 ACCGGT A^CCGGT
AocI 4028 CCTCAGG CC^T[ACGT]AGG
AseI 4499 ATTAAT AT^TAAT
AsnI 4499 ATTAAT AT^TAAT
Asp700I 1616 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3095 GGTACC G^GTACC
AviII 1333 TGCGCA TGC^GCA
BclI 776 TGATCA T^GATCA
BglII 2239 AGATCT A^GATCT
BsaAI 162 TACGTG [CT]AC^GT[GA]
BsiEI 872 CGGTCG CG[GA][CT]^CG
BsmBI 1104 CGTCTC CGTCTC(1/5)
BstAPI 2519 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 4028 CCTCAGG CC^T[ACGT]AGG
DraIII 4204 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4028 CCTCAGG CC^T[ACGT]AGG
EcoRI 1588 GAATTC G^AATTC
FbaI 776 TGATCA T^GATCA
FspI 1333 TGCGCA TGC^GCA
KpnI 3095 GGTACC GGTAC^C
PaeR7I 2458 CTCGAG C^TCGAG
PinAI 1687 ACCGGT A^CCGGT
PshBI 4499 ATTAAT AT^TAAT
PstI 840 CTGCAG CTGCA^G
SapI 1604 GCTCTTC GCTCTTC(1/4)
SbfI 839 CCTGCAGG CCTGCA^GG
SfiI 365 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 849 CCCGGG CCC^GGG
Sse8387I 839 CCTGCAGG CCTGCA^GG
SspI 4581 AATATT AAT^ATT
XhoI 2458 CTCGAG C^TCGAG
XmaCI 849 CCCGGG C^CCGGG
XmaI 849 CCCGGG C^CCGGG
XmnI 1616 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 1963 CGCG CG^CG
2101 CGCG
AcsI 17 GAATTT [GA]^AATT[CT]
1588 GAATTC
AflIII 1897 ACATGT A^C[GA][CT]GT
3196 ACATGT
ApoI 17 GAATTT [GA]^AATT[CT]
1588 GAATTC
AvrII 3125 CCTAGG C^CTAGG
3230 CCTAGG
BalI 2191 TGGCCA TGG^CCA
4416 TGGCCA
BamHI 4061 GGATCC G^GATCC
4163 GGATCC
BcgI 445 CGATGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2027 CGATGTAATTGC
BlnI 3125 CCTAGG C^CTAGG
3230 CCTAGG
BspLU11I 1897 ACATGT A^CATGT
3196 ACATGT
BsrDI 2453 CATTGC GCAATG(2/0)
3205 GCAATG
BssSI 4285 CACGAG CACGAG(-5/-1)
192 CTCGTG
BstUI 1963 CGCG CG^CG
2101 CGCG
DrdI 1143 GACCATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1517 GACTCTGATGTC
MluNI 2191 TGGCCA TGG^CCA
4416 TGGCCA
MscI 2191 TGGCCA TGG^CCA
4416 TGGCCA
MvnI 1963 CGCG CG^CG
2101 CGCG
NcoI 1399 CCATGG C^CATGG
2194 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI Aor51HI ApaI AspEI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII