##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AspEI AspI AviII BbeI BseAI
BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BstBI BstXI Cfr10I CpoI
EagI Eam1105I EclXI Eco52I FseI
FspI HindIII HpaI KasI KspI
MluI MroI NaeI NarI NgoMIV
NotI NruI NspV PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1316 GACGTC GACGT^C
Acc65I 1491 GGTACC G^GTACC
AflII 4837 CTTAAG C^TTAAG
Aor51HI 633 AGCGCT AGC^GCT
AseI 5576 ATTAAT AT^TAAT
AsnI 5576 ATTAAT AT^TAAT
Asp718I 1491 GGTACC G^GTACC
BbrPI 3350 CACGTG CAC^GTG
BbvCI 4823 CCTCAGC CCTCAGC(-5/-2)
BcgI 727 GCATCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5400 TGATCA T^GATCA
BfrI 4837 CTTAAG C^TTAAG
BglII 6014 AGATCT A^GATCT
BsaBI 2200 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4722 ACCGGA [AT]^CCGG[AT]
BspLU11I 921 ACATGT A^CATGT
Bst1107I 3246 GTATAC GTA^TAC
BstEII 883 GGTCACC G^GT[ACGT]ACC
BstPI 883 GGTCACC G^GT[ACGT]ACC
BstZ17I 3246 GTATAC GTA^TAC
DraIII 5602 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 633 AGCGCT AGC^GCT
EcoNI 2553 CCTCTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 883 GGTCACC G^GT[ACGT]ACC
FbaI 5400 TGATCA T^GATCA
KpnI 1491 GGTACC GGTAC^C
MamI 2200 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6 CAATTG C^AATTG
MunI 6 CAATTG C^AATTG
NdeI 2733 CATATG CA^TATG
NheI 6814 GCTAGC G^CTAGC
PacI 5553 TTAATTAA TTAAT^TAA
PmaCI 3350 CACGTG CAC^GTG
PmlI 3350 CACGTG CAC^GTG
PshBI 5576 ATTAAT AT^TAAT
SacI 893 GAGCTC GAGCT^C
SapI 4924 GCTCTTC GCTCTTC(1/4)
SpeI 5508 ACTAGT A^CTAGT
SphI 3875 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 540 CGCG CG^CG
2219 CGCG
AcyI 1316 GACGTC G[GA]^CG[CT]C
1616 GACGCC
AocI 2123 CCTAAGG CC^T[ACGT]AGG
5906 CCTAAGG
ApaI 2324 GGGCCC GGGCC^C
4419 GGGCCC
BciVI 4682 GGATAC GTATCC(6/5)
4612 GTATCC
BsaHI 1316 GACGTC G[GA]^CG[CT]C
1616 GACGCC
Bsp1407I 3380 TGTACA T^GTACA
5285 TGTACA
BspDI 1214 ATCGAT AT^CGAT
5253 ATCGAT
BsrBI 2527 CCGCTC CCGCTC(-3/-3)
5950 GAGCGG
BsrGI 3380 TGTACA T^GTACA
5285 TGTACA
BstAPI 547 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
556 GCAAGACTTGC
BstUI 540 CGCG CG^CG
2219 CGCG
Bsu36I 2123 CCTAAGG CC^T[ACGT]AGG
5906 CCTAAGG
ClaI 1214 ATCGAT AT^CGAT
5253 ATCGAT
Eco81I 2123 CCTAAGG CC^T[ACGT]AGG
5906 CCTAAGG
HgaI 1616 GACGC GACGC(5/10)
2837 GACGC
Hin1I 1316 GACGTC G[GA]^CG[CT]C
1616 GACGCC
HincII 3191 GTCAAC GT[CT]^[GA]AC
4768 GTTGAC
HindII 3191 GTCAAC GT[CT]^[GA]AC
4768 GTTGAC
MvnI 540 CGCG CG^CG
2219 CGCG
NcoI 2371 CCATGG C^CATGG
6585 CCATGG
SmaI 268 CCCGGG CCC^GGG
660 CCCGGG
SnaBI 2691 TACGTA TAC^GTA
2741 TACGTA
SspBI 3380 TGTACA T^GTACA
5285 TGTACA
SspI 3409 AATATT AAT^ATT
4668 AATATT
StuI 2394 AGGCCT AGG^CCT
4476 AGGCCT
XmaCI 268 CCCGGG C^CCGGG
660 CCCGGG
XmaI 268 CCCGGG C^CCGGG
660 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI