##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII BamHI BciVI BclI
BglII BsaBI BseAI BsiEI BsiWI
BsmI BspEI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CpoI EagI
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI FspI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Aor51HI 1955 AGCGCT AGC^GCT
Asp700I 3057 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3861 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1448 GGCGCC GGCGC^C
BbsI 4928 GTCTTC GAAGAC(2/6)
BcgI 1434 CGATGCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1456 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1456 GCTCAGC GC^T[ACGT]AGC
BpuAI 4928 GTCTTC GAAGAC(2/6)
BsaWI 11 ACCGGA [AT]^CCGG[AT]
BsmBI 235 GAGACG CGTCTC(1/5)
Bsp1407I 4431 TGTACA T^GTACA
BspDI 1039 ATCGAT AT^CGAT
BspHI 314 TCATGA T^CATGA
BsrDI 2552 GCAATG GCAATG(2/0)
BsrFI 491 GCCGGC [GA]^CCGG[CT]
BsrGI 4431 TGTACA T^GTACA
CelII 1456 GCTCAGC GC^T[ACGT]AGC
Cfr10I 491 GCCGGC [GA]^CCGG[CT]
ClaI 1039 ATCGAT AT^CGAT
DraI 2227 TTTAAA TTT^AAA
DraIII 2299 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3530 GACAGACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1955 AGCGCT AGC^GCT
EcoRV 1728 GATATC GAT^ATC
HpaI 4956 GTTAAC GTT^AAC
KasI 1448 GGCGCC G^GCGCC
NaeI 491 GCCGGC GCC^GGC
NarI 1448 GGCGCC GG^CGCC
NgoMIV 491 GCCGGC G^CCGGC
PaeR7I 1809 CTCGAG C^TCGAG
PflFI 3861 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3171 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 314 TCATGA T^CATGA
SfoI 1448 GGCGCC GGC^GCC
SmaI 974 CCCGGG CCC^GGG
SpeI 5463 ACTAGT A^CTAGT
SspBI 4431 TGTACA T^GTACA
SspI 3200 AATATT AAT^ATT
Tth111I 3861 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 156 TCTAGA T^CTAGA
XhoI 1809 CTCGAG C^TCGAG
XmaCI 974 CCCGGG C^CCGGG
XmaI 974 CCCGGG C^CCGGG
XmnI 3057 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 4296 GTAGAC GT^[AC][GT]AC
4620 GTCTAC
AccII 90 CGCG CG^CG
783 CGCG
AcyI 1246 GACGCC G[GA]^CG[CT]C
1448 GGCGCC
AhdI 3029 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3990 GACCACGGGTC
AspEI 3029 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3990 GACCACGGGTC
BbvCI 2541 GCTGAGG CCTCAGC(-5/-2)
5085 GCTGAGG
BsaHI 1246 GACGCC G[GA]^CG[CT]C
1448 GGCGCC
BstUI 90 CGCG CG^CG
783 CGCG
Eam1105I 3029 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3990 GACCACGGGTC
EcoNI 2247 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2363 CCTCTAACAGG
EcoRI 3463 GAATTC G^AATTC
4206 GAATTC
Hin1I 1246 GACGCC G[GA]^CG[CT]C
1448 GGCGCC
HindIII 1903 AAGCTT A^AGCTT
3299 AAGCTT
MvnI 90 CGCG CG^CG
783 CGCG
ScaI 530 AGTACT AGT^ACT
3307 AGTACT
SphI 1133 GCATGC GCATG^C
4007 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BfrI BglI BlnI
BmyI BpmI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII