##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BclI BsaWI BseAI BspDI
BspEI BspLU11I Bst1107I BstBI BstZ17I
ClaI Eam1105I Eco47III EcoRV FbaI
FseI HpaI MroI NotI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI SalI SfiI SfuI
SgrAI SmaI SnaBI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 622 GTCTAC GT^[AC][GT]AC
AflII 789 CTTAAG C^TTAAG
Alw44I 4626 GTGCAC G^TGCAC
AocI 3355 CCTGAGG CC^T[ACGT]AGG
ApaLI 4626 GTGCAC G^TGCAC
BamHI 3929 GGATCC G^GATCC
BfrI 789 CTTAAG C^TTAAG
BlpI 5562 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5562 GCTAAGC GC^T[ACGT]AGC
BsaBI 1973 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2022 CGTACG C^GTACG
Bsp1407I 5573 TGTACA T^GTACA
BsrGI 5573 TGTACA T^GTACA
BssSI 186 CACGAG CACGAG(-5/-1)
Bsu36I 3355 CCTGAGG CC^T[ACGT]AGG
CelII 5562 GCTAAGC GC^T[ACGT]AGC
CpoI 6228 CGGACCG CG^G[AT]CCG
DrdI 2027 GACCGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 223 CGGCCG C^GGCCG
EclXI 223 CGGCCG C^GGCCG
Eco52I 223 CGGCCG C^GGCCG
Eco81I 3355 CCTGAGG CC^T[ACGT]AGG
EcoRI 5879 GAATTC G^AATTC
EcoT22I 6085 ATGCAT ATGCA^T
HindIII 4461 AAGCTT A^AGCTT
KspI 1814 CCGCGG CCGC^GG
MamI 1973 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6313 CAATTG C^AATTG
MluI 204 ACGCGT A^CGCGT
MunI 6313 CAATTG C^AATTG
NdeI 6082 CATATG CA^TATG
NheI 1572 GCTAGC G^CTAGC
NruI 1487 TCGCGA TCG^CGA
NsiI 6085 ATGCAT ATGCA^T
PaeR7I 285 CTCGAG C^TCGAG
PpuMI 3359 AGGTCCT [GA]G^G[AT]CC[CT]
RsrII 6228 CGGACCG CG^G[AT]CCG
SacII 1814 CCGCGG CCGC^GG
SspBI 5573 TGTACA T^GTACA
SspI 5961 AATATT AAT^ATT
XhoI 285 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3938 GGTACC G^GTACC
5393 GGTACC
Asp700I 2938 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3141 GAAGAGTTTC
Asp718I 3938 GGTACC G^GTACC
5393 GGTACC
AvaI 285 CTCGAG C^[CT]CG[GA]G
1228 CTCGGG
AvrII 3106 CCTAGG C^CTAGG
3363 CCTAGG
BbrPI 1419 CACGTG CAC^GTG
4001 CACGTG
BcgI 4516 CGAAGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3030 GCATGTTGCTCG
BciVI 3642 GGATAC GTATCC(6/5)
4833 GTATCC
BlnI 3106 CCTAGG C^CTAGG
3363 CCTAGG
BsiEI 223 CGGCCG CG[GA][CT]^CG
2026 CGACCG
BsoBI 285 CTCGAG C^[CT]CG[GA]G
1228 CTCGGG
BspHI 3137 TCATGA T^CATGA
3858 TCATGA
BsrDI 5279 CATTGC GCAATG(2/0)
6008 GCAATG
BssHII 83 GCGCGC G^CGCGC
161 GCGCGC
BstAPI 1151 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3454 GCAGCTTGTGC
DraI 4901 TTTAAA TTT^AAA
6073 TTTAAA
HincII 4742 GTTGAC GT[CT]^[GA]AC
4752 GTCAAC
HindII 4742 GTTGAC GT[CT]^[GA]AC
4752 GTCAAC
KpnI 3938 GGTACC GGTAC^C
5393 GGTACC
NaeI 73 GCCGGC GCC^GGC
5616 GCCGGC
NgoMIV 73 GCCGGC G^CCGGC
5616 GCCGGC
PmaCI 1419 CACGTG CAC^GTG
4001 CACGTG
PmlI 1419 CACGTG CAC^GTG
4001 CACGTG
RcaI 3137 TCATGA T^CATGA
3858 TCATGA
ScaI 430 AGTACT AGT^ACT
6021 AGTACT
SpeI 3518 ACTAGT A^CTAGT
5803 ACTAGT
StuI 3662 AGGCCT AGG^CCT
4151 AGGCCT
XmnI 2938 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3141 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaII AviII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI Sse8387I StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII