################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccIII AclI AscI AseI AsnI Asp700I BcgI BclI BglII BsaBI BseAI BsiWI BspDI BspEI BspHI BssHII BstBI BstXI ClaI CpoI DrdI EcoRV FbaI FseI HincII HindII HpaI KspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SnaBI Sse8387I SspI SwaI Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 3403 GACGTC GACGT^C AflII 2262 CTTAAG C^TTAAG AgeI 4968 ACCGGT A^CCGGT AhdI 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2337 GTGCAC G^TGCAC Aor51HI 1320 AGCGCT AGC^GCT ApaLI 2337 GTGCAC G^TGCAC AspEI 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1491 TGCGCA TGC^GCA BamHI 1004 GGATCC G^GATCC BbeI 5247 GGCGCC GGCGC^C BbrPI 3505 CACGTG CAC^GTG BfrI 2262 CTTAAG C^TTAAG BsiEI 1430 CGGCCG CG[GA][CT]^CG BsmBI 3405 CGTCTC CGTCTC(1/5) BssSI 5084 CACGAG CACGAG(-5/-1) BstEII 5925 GGTAACC G^GT[ACGT]ACC BstPI 5925 GGTAACC G^GT[ACGT]ACC EagI 1430 CGGCCG C^GGCCG Eam1105I 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1430 CGGCCG C^GGCCG Eco47III 1320 AGCGCT AGC^GCT Eco52I 1430 CGGCCG C^GGCCG EcoO65I 5925 GGTAACC G^GT[ACGT]ACC EcoRI 912 GAATTC G^AATTC FspI 1491 TGCGCA TGC^GCA KasI 5247 GGCGCC G^GCGCC MfeI 3189 CAATTG C^AATTG MunI 3189 CAATTG C^AATTG NarI 5247 GGCGCC GG^CGCC PflFI 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4968 ACCGGT A^CCGGT PmaCI 3505 CACGTG CAC^GTG PmlI 3505 CACGTG CAC^GTG PshAI 2004 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 2497 ACCAGGT A^CC[AT]GGT SfoI 5247 GGCGCC GGC^GCC SgrAI 1088 CGCCGGTG C[GA]^CCGG[CT]G TaqI 3511 TCGA T^CGA Tth111I 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 3511 TCGA T^CGA XbaI 6107 TCTAGA T^CTAGA XcmI 2976 CCACGTAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 883 GGTACC G^GTACC 4826 GGTACC AccII 1182 CGCG CG^CG 5994 CGCG ApaI 3663 GGGCCC GGGCC^C 5989 GGGCCC Asp718I 883 GGTACC G^GTACC 4826 GGTACC AvrII 1395 CCTAGG C^CTAGG 5333 CCTAGG BbsI 1146 GTCTTC GAAGAC(2/6) 4415 GTCTTC BglI 319 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5165 GCCACCGAGGC BlnI 1395 CCTAGG C^CTAGG 5333 CCTAGG BlpI 4123 GCTCAGC GC^T[ACGT]AGC 4297 GCTGAGC Bpu1102I 4123 GCTCAGC GC^T[ACGT]AGC 4297 GCTGAGC BpuAI 1146 GTCTTC GAAGAC(2/6) 4415 GTCTTC BsaAI 2977 CACGTA [CT]AC^GT[GA] 3505 CACGTG Bsp1407I 2021 TGTACA T^GTACA 6150 TGTACA BsrGI 2021 TGTACA T^GTACA 6150 TGTACA Bst1107I 535 GTATAC GTA^TAC 2554 GTATAC BstUI 1182 CGCG CG^CG 5994 CGCG BstZ17I 535 GTATAC GTA^TAC 2554 GTATAC CelII 4123 GCTCAGC GC^T[ACGT]AGC 4297 GCTGAGC EcoT22I 3536 ATGCAT ATGCA^T 4709 ATGCAT HindIII 1235 AAGCTT A^AGCTT 3347 AAGCTT KpnI 883 GGTACC GGTAC^C 4826 GGTACC MvnI 1182 CGCG CG^CG 5994 CGCG NdeI 4635 CATATG CA^TATG 4902 CATATG NsiI 3536 ATGCAT ATGCA^T 4709 ATGCAT PpuMI 66 AGGTCCC [GA]G^G[AT]CC[CT] 2455 AGGACCT SapI 3024 GAAGAGC GCTCTTC(1/4) 4072 GAAGAGC ScaI 2982 AGTACT AGT^ACT 3735 AGTACT SmaI 1110 CCCGGG CCC^GGG 3666 CCCGGG SpeI 4717 ACTAGT A^CTAGT 5341 ACTAGT SspBI 2021 TGTACA T^GTACA 6150 TGTACA XmaCI 1110 CCCGGG C^CCGGG 3666 CCCGGG XmaI 1110 CCCGGG C^CCGGG 3666 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII