##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AscI AseI AsnI
Asp700I BcgI BclI BglII BsaBI
BseAI BsiWI BspDI BspEI BspHI
BssHII BstBI BstXI ClaI CpoI
DrdI EcoRV FbaI FseI HincII
HindII HpaI KspI MamI MluI
MroI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfuI SnaBI Sse8387I SspI SwaI
Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3403 GACGTC GACGT^C
AflII 2262 CTTAAG C^TTAAG
AgeI 4968 ACCGGT A^CCGGT
AhdI 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2337 GTGCAC G^TGCAC
Aor51HI 1320 AGCGCT AGC^GCT
ApaLI 2337 GTGCAC G^TGCAC
AspEI 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1491 TGCGCA TGC^GCA
BamHI 1004 GGATCC G^GATCC
BbeI 5247 GGCGCC GGCGC^C
BbrPI 3505 CACGTG CAC^GTG
BfrI 2262 CTTAAG C^TTAAG
BsiEI 1430 CGGCCG CG[GA][CT]^CG
BsmBI 3405 CGTCTC CGTCTC(1/5)
BssSI 5084 CACGAG CACGAG(-5/-1)
BstEII 5925 GGTAACC G^GT[ACGT]ACC
BstPI 5925 GGTAACC G^GT[ACGT]ACC
EagI 1430 CGGCCG C^GGCCG
Eam1105I 4907 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1430 CGGCCG C^GGCCG
Eco47III 1320 AGCGCT AGC^GCT
Eco52I 1430 CGGCCG C^GGCCG
EcoO65I 5925 GGTAACC G^GT[ACGT]ACC
EcoRI 912 GAATTC G^AATTC
FspI 1491 TGCGCA TGC^GCA
KasI 5247 GGCGCC G^GCGCC
MfeI 3189 CAATTG C^AATTG
MunI 3189 CAATTG C^AATTG
NarI 5247 GGCGCC GG^CGCC
PflFI 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4968 ACCGGT A^CCGGT
PmaCI 3505 CACGTG CAC^GTG
PmlI 3505 CACGTG CAC^GTG
PshAI 2004 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2497 ACCAGGT A^CC[AT]GGT
SfoI 5247 GGCGCC GGC^GCC
SgrAI 1088 CGCCGGTG C[GA]^CCGG[CT]G
TaqI 3511 TCGA T^CGA
Tth111I 2783 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3511 TCGA T^CGA
XbaI 6107 TCTAGA T^CTAGA
XcmI 2976 CCACGTAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 883 GGTACC G^GTACC
4826 GGTACC
AccII 1182 CGCG CG^CG
5994 CGCG
ApaI 3663 GGGCCC GGGCC^C
5989 GGGCCC
Asp718I 883 GGTACC G^GTACC
4826 GGTACC
AvrII 1395 CCTAGG C^CTAGG
5333 CCTAGG
BbsI 1146 GTCTTC GAAGAC(2/6)
4415 GTCTTC
BglI 319 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5165 GCCACCGAGGC
BlnI 1395 CCTAGG C^CTAGG
5333 CCTAGG
BlpI 4123 GCTCAGC GC^T[ACGT]AGC
4297 GCTGAGC
Bpu1102I 4123 GCTCAGC GC^T[ACGT]AGC
4297 GCTGAGC
BpuAI 1146 GTCTTC GAAGAC(2/6)
4415 GTCTTC
BsaAI 2977 CACGTA [CT]AC^GT[GA]
3505 CACGTG
Bsp1407I 2021 TGTACA T^GTACA
6150 TGTACA
BsrGI 2021 TGTACA T^GTACA
6150 TGTACA
Bst1107I 535 GTATAC GTA^TAC
2554 GTATAC
BstUI 1182 CGCG CG^CG
5994 CGCG
BstZ17I 535 GTATAC GTA^TAC
2554 GTATAC
CelII 4123 GCTCAGC GC^T[ACGT]AGC
4297 GCTGAGC
EcoT22I 3536 ATGCAT ATGCA^T
4709 ATGCAT
HindIII 1235 AAGCTT A^AGCTT
3347 AAGCTT
KpnI 883 GGTACC GGTAC^C
4826 GGTACC
MvnI 1182 CGCG CG^CG
5994 CGCG
NdeI 4635 CATATG CA^TATG
4902 CATATG
NsiI 3536 ATGCAT ATGCA^T
4709 ATGCAT
PpuMI 66 AGGTCCC [GA]G^G[AT]CC[CT]
2455 AGGACCT
SapI 3024 GAAGAGC GCTCTTC(1/4)
4072 GAAGAGC
ScaI 2982 AGTACT AGT^ACT
3735 AGTACT
SmaI 1110 CCCGGG CCC^GGG
3666 CCCGGG
SpeI 4717 ACTAGT A^CTAGT
5341 ACTAGT
SspBI 2021 TGTACA T^GTACA
6150 TGTACA
XmaCI 1110 CCCGGG C^CCGGG
3666 CCCGGG
XmaI 1110 CCCGGG C^CCGGG
3666 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII