##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
Alw44I ApaLI AscI AspI BbrPI
BsaAI BsiWI BspDI BspHI Bst1107I
BstZ17I ClaI DraIII EcoRV EcoT22I
FseI HpaI MfeI MluI MunI
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmlI PpuMI PshAI
PvuI RcaI SalI SfiI SnaBI
SpeI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3067 GACGTC GACGT^C
AccI 1154 GTAGAC GT^[AC][GT]AC
AclI 4953 AACGTT AA^CGTT
AflIII 1695 ACATGT A^C[GA][CT]GT
AgeI 4741 ACCGGT A^CCGGT
AocI 2284 CCTGAGG CC^T[ACGT]AGG
AseI 713 ATTAAT AT^TAAT
AsnI 713 ATTAAT AT^TAAT
AvrII 4518 CCTAGG C^CTAGG
BamHI 3766 GGATCC G^GATCC
BbeI 370 GGCGCC GGCGC^C
BcgI 5338 GCAATTTAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2802 TGATCA T^GATCA
BlnI 4518 CCTAGG C^CTAGG
BsgI 3312 GTGCAG GTGCAG(16/14)
Bsp1407I 4597 TGTACA T^GTACA
BspLU11I 1695 ACATGT A^CATGT
BsrGI 4597 TGTACA T^GTACA
BstAPI 975 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2441 TTCGAA TT^CGAA
BstEII 289 GGTGACC G^GT[ACGT]ACC
BstPI 289 GGTGACC G^GT[ACGT]ACC
Bsu36I 2284 CCTGAGG CC^T[ACGT]AGG
CpoI 3918 CGGTCCG CG^G[AT]CCG
DrdI 5237 GACGCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1450 CGGCCG C^GGCCG
EclXI 1450 CGGCCG C^GGCCG
Eco52I 1450 CGGCCG C^GGCCG
Eco81I 2284 CCTGAGG CC^T[ACGT]AGG
EcoO65I 289 GGTGACC G^GT[ACGT]ACC
FbaI 2802 TGATCA T^GATCA
KasI 370 GGCGCC G^GCGCC
NarI 370 GGCGCC GG^CGCC
NdeI 2912 CATATG CA^TATG
NheI 1547 GCTAGC G^CTAGC
NspV 2441 TTCGAA TT^CGAA
PinAI 4741 ACCGGT A^CCGGT
PmeI 5413 GTTTAAAC GTTT^AAAC
PshBI 713 ATTAAT AT^TAAT
Psp1406I 4953 AACGTT AA^CGTT
RsrII 3918 CGGTCCG CG^G[AT]CCG
SapI 3038 GAAGAGC GCTCTTC(1/4)
SbfI 2336 CCTGCAGG CCTGCA^GG
SexAI 1464 ACCTGGT A^CC[AT]GGT
SfoI 370 GGCGCC GGC^GCC
SfuI 2441 TTCGAA TT^CGAA
SgrAI 410 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2526 GCATGC GCATG^C
Sse8387I 2336 CCTGCAGG CCTGCA^GG
SspBI 4597 TGTACA T^GTACA
SspI 3707 AATATT AAT^ATT
XbaI 727 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1306 GGTACC G^GTACC
4567 GGTACC
AccIII 2268 TCCGGA T^CCGGA
3364 TCCGGA
AflII 4783 CTTAAG C^TTAAG
4931 CTTAAG
AhdI 1106 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4363 GACTTACCGTC
Asp718I 1306 GGTACC G^GTACC
4567 GGTACC
AspEI 1106 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4363 GACTTACCGTC
BciVI 3018 GGATAC GTATCC(6/5)
3279 GTATCC
BfrI 4783 CTTAAG C^TTAAG
4931 CTTAAG
BglII 2235 AGATCT A^GATCT
3960 AGATCT
BsaI 3649 GAGACC GGTCTC(1/5)
833 GGTCTC
BseAI 2268 TCCGGA T^CCGGA
3364 TCCGGA
BsiEI 1450 CGGCCG CG[GA][CT]^CG
2597 CGGTCG
BsmI 5021 GAATGC GAATGC(1/-1)
5276 GCATTC
BspEI 2268 TCCGGA T^CCGGA
3364 TCCGGA
BssHII 2389 GCGCGC G^CGCGC
3907 GCGCGC
BssSI 2143 CACGAG CACGAG(-5/-1)
543 CTCGTG
Eam1105I 1106 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4363 GACTTACCGTC
EcoNI 128 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4757 CCTCTGTAAGG
EcoRI 1195 GAATTC G^AATTC
4298 GAATTC
KpnI 1306 GGTACC GGTAC^C
4567 GGTACC
KspI 107 CCGCGG CCGC^GG
3558 CCGCGG
MroI 2268 TCCGGA T^CCGGA
3364 TCCGGA
SacII 107 CCGCGG CCGC^GG
3558 CCGCGG
ScaI 1764 AGTACT AGT^ACT
2010 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI Asp700I AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI