##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I ApaLI AscI AspEI BbeI
BbrPI BbvCI BfrI BglI BsaAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI KasI
KspI MfeI MluI MroI MunI
NarI NotI NruI NsiI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SspBI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 5362 GGTACC G^GTACC
AccII 220 CGCG CG^CG
AclI 2932 AACGTT AA^CGTT
AcyI 1155 GACGCC G[GA]^CG[CT]C
AflIII 2110 ACATGT A^C[GA][CT]GT
AocI 5526 CCTTAGG CC^T[ACGT]AGG
Aor51HI 580 AGCGCT AGC^GCT
AseI 3552 ATTAAT AT^TAAT
AsnI 3552 ATTAAT AT^TAAT
Asp718I 5362 GGTACC G^GTACC
AspI 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 5362 GGTACC G^G[CT][GA]CC
BlpI 4823 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4823 GCTCAGC GC^T[ACGT]AGC
BsaHI 1155 GACGCC G[GA]^CG[CT]C
BsaWI 3375 ACCGGA [AT]^CCGG[AT]
BspLU11I 2110 ACATGT A^CATGT
BsrBI 807 GAGCGG CCGCTC(-3/-3)
BsrFI 1549 GCCGGC [GA]^CCGG[CT]
BssSI 5869 CACGAG CACGAG(-5/-1)
BstAPI 4099 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2936 TTCGAA TT^CGAA
BstEII 5718 GGTTACC G^GT[ACGT]ACC
BstPI 5718 GGTTACC G^GT[ACGT]ACC
BstUI 220 CGCG CG^CG
Bsu36I 5526 CCTTAGG CC^T[ACGT]AGG
CelII 4823 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1549 GCCGGC [GA]^CCGG[CT]
DrdI 5886 GACTCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 580 AGCGCT AGC^GCT
Eco81I 5526 CCTTAGG CC^T[ACGT]AGG
EcoO65I 5718 GGTTACC G^GT[ACGT]ACC
FauI 3323 GCGGG CCCGC(4/6)
Hin1I 1155 GACGCC G[GA]^CG[CT]C
HindIII 3052 AAGCTT A^AGCTT
HpaI 1008 GTTAAC GTT^AAC
KpnI 5362 GGTACC GGTAC^C
MvnI 220 CGCG CG^CG
NaeI 1549 GCCGGC GCC^GGC
NdeI 757 CATATG CA^TATG
NgoMIV 1549 GCCGGC G^CCGGC
NspV 2936 TTCGAA TT^CGAA
PacI 5420 TTAATTAA TTAAT^TAA
PflFI 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3552 ATTAAT AT^TAAT
Psp1406I 2932 AACGTT AA^CGTT
SfuI 2936 TTCGAA TT^CGAA
SmaI 2498 CCCGGG CCC^GGG
Tth111I 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2498 CCCGGG C^CCGGG
XmaI 2498 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AviII 1342 TGCGCA TGC^GCA
3650 TGCGCA
BalI 3173 TGGCCA TGG^CCA
4619 TGGCCA
BamHI 1744 GGATCC G^GATCC
4007 GGATCC
BciVI 1947 GGATAC GTATCC(6/5)
6447 GTATCC
BclI 104 TGATCA T^GATCA
2914 TGATCA
BglII 796 AGATCT A^GATCT
4816 AGATCT
BsaI 3750 GAGACC GGTCTC(1/5)
6080 GGTCTC
BsmBI 222 CGTCTC CGTCTC(1/5)
916 CGTCTC
EcoRI 3002 GAATTC G^AATTC
3952 GAATTC
FbaI 104 TGATCA T^GATCA
2914 TGATCA
FspI 1342 TGCGCA TGC^GCA
3650 TGCGCA
MluNI 3173 TGGCCA TGG^CCA
4619 TGGCCA
MscI 3173 TGGCCA TGG^CCA
4619 TGGCCA
NspI 1519 GCATGT [GA]CATG^[CT]
2110 ACATGT
PshAI 2219 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3973 GACTGAAGTC
SacI 2140 GAGCTC GAGCT^C
4680 GAGCTC
SpeI 3521 ACTAGT A^CTAGT
4940 ACTAGT
SspI 3581 AATATT AAT^ATT
3613 AATATT
TaqI 523 TCGA T^CGA
2937 TCGA
TthHB8I 523 TCGA T^CGA
2937 TCGA
XbaI 3006 TCTAGA T^CTAGA
4256 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII AvrII BanII
BbsI BbvI BcgI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NheI NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmnI