################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AflII AgeI AhdI Alw44I ApaLI AscI AspEI BbeI BbrPI BbvCI BfrI BglI BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI KasI KspI MfeI MluI MroI MunI NarI NotI NruI NsiI PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 5362 GGTACC G^GTACC AccII 220 CGCG CG^CG AclI 2932 AACGTT AA^CGTT AcyI 1155 GACGCC G[GA]^CG[CT]C AflIII 2110 ACATGT A^C[GA][CT]GT AocI 5526 CCTTAGG CC^T[ACGT]AGG Aor51HI 580 AGCGCT AGC^GCT AseI 3552 ATTAAT AT^TAAT AsnI 3552 ATTAAT AT^TAAT Asp718I 5362 GGTACC G^GTACC AspI 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 5362 GGTACC G^G[CT][GA]CC BlpI 4823 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4823 GCTCAGC GC^T[ACGT]AGC BsaHI 1155 GACGCC G[GA]^CG[CT]C BsaWI 3375 ACCGGA [AT]^CCGG[AT] BspLU11I 2110 ACATGT A^CATGT BsrBI 807 GAGCGG CCGCTC(-3/-3) BsrFI 1549 GCCGGC [GA]^CCGG[CT] BssSI 5869 CACGAG CACGAG(-5/-1) BstAPI 4099 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2936 TTCGAA TT^CGAA BstEII 5718 GGTTACC G^GT[ACGT]ACC BstPI 5718 GGTTACC G^GT[ACGT]ACC BstUI 220 CGCG CG^CG Bsu36I 5526 CCTTAGG CC^T[ACGT]AGG CelII 4823 GCTCAGC GC^T[ACGT]AGC Cfr10I 1549 GCCGGC [GA]^CCGG[CT] DrdI 5886 GACTCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 580 AGCGCT AGC^GCT Eco81I 5526 CCTTAGG CC^T[ACGT]AGG EcoO65I 5718 GGTTACC G^GT[ACGT]ACC FauI 3323 GCGGG CCCGC(4/6) Hin1I 1155 GACGCC G[GA]^CG[CT]C HindIII 3052 AAGCTT A^AGCTT HpaI 1008 GTTAAC GTT^AAC KpnI 5362 GGTACC GGTAC^C MvnI 220 CGCG CG^CG NaeI 1549 GCCGGC GCC^GGC NdeI 757 CATATG CA^TATG NgoMIV 1549 GCCGGC G^CCGGC NspV 2936 TTCGAA TT^CGAA PacI 5420 TTAATTAA TTAAT^TAA PflFI 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3552 ATTAAT AT^TAAT Psp1406I 2932 AACGTT AA^CGTT SfuI 2936 TTCGAA TT^CGAA SmaI 2498 CCCGGG CCC^GGG Tth111I 4283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2498 CCCGGG C^CCGGG XmaI 2498 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AviII 1342 TGCGCA TGC^GCA 3650 TGCGCA BalI 3173 TGGCCA TGG^CCA 4619 TGGCCA BamHI 1744 GGATCC G^GATCC 4007 GGATCC BciVI 1947 GGATAC GTATCC(6/5) 6447 GTATCC BclI 104 TGATCA T^GATCA 2914 TGATCA BglII 796 AGATCT A^GATCT 4816 AGATCT BsaI 3750 GAGACC GGTCTC(1/5) 6080 GGTCTC BsmBI 222 CGTCTC CGTCTC(1/5) 916 CGTCTC EcoRI 3002 GAATTC G^AATTC 3952 GAATTC FbaI 104 TGATCA T^GATCA 2914 TGATCA FspI 1342 TGCGCA TGC^GCA 3650 TGCGCA MluNI 3173 TGGCCA TGG^CCA 4619 TGGCCA MscI 3173 TGGCCA TGG^CCA 4619 TGGCCA NspI 1519 GCATGT [GA]CATG^[CT] 2110 ACATGT PshAI 2219 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3973 GACTGAAGTC SacI 2140 GAGCTC GAGCT^C 4680 GAGCTC SpeI 3521 ACTAGT A^CTAGT 4940 ACTAGT SspI 3581 AATATT AAT^ATT 3613 AATATT TaqI 523 TCGA T^CGA 2937 TCGA TthHB8I 523 TCGA T^CGA 2937 TCGA XbaI 3006 TCTAGA T^CTAGA 4256 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII AvrII BanII BbsI BbvI BcgI BcnI BfaI BlnI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmnI