##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflIII AgeI Aor51HI
AscI AseI AsnI AviII BbrPI
BbvCI BclI BglI BsaBI BsiEI
BsiWI BspHI BspLU11I BssHII BstBI
CpoI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HgaI HpaI KspI MamI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 454 GGTACC G^GTACC
AcyI 96 GGCGCC G[GA]^CG[CT]C
AflII 3895 CTTAAG C^TTAAG
AhdI 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 879 GTGCAC G^TGCAC
ApaI 3890 GGGCCC GGGCC^C
ApaLI 879 GTGCAC G^TGCAC
Asp718I 454 GGTACC G^GTACC
AspEI 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 96 GGCGCC GGCGC^C
BfrI 3895 CTTAAG C^TTAAG
BglII 4212 AGATCT A^GATCT
BsaAI 3925 TACGTG [CT]AC^GT[GA]
BsaHI 96 GGCGCC G[GA]^CG[CT]C
BsaI 3016 GAGACC GGTCTC(1/5)
BsmBI 1193 GAGACG CGTCTC(1/5)
BsmI 3670 GCATTC GAATGC(1/-1)
BspDI 4559 ATCGAT AT^CGAT
BsrBI 1099 GAGCGG CCGCTC(-3/-3)
Bst1107I 1416 GTATAC GTA^TAC
BstEII 801 GGTGACC G^GT[ACGT]ACC
BstPI 801 GGTGACC G^GT[ACGT]ACC
BstZ17I 1416 GTATAC GTA^TAC
ClaI 4559 ATCGAT AT^CGAT
DraI 3095 TTTAAA TTT^AAA
DrdI 637 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4072 CCATGG C^C[GA][CT]GG
Eam1105I 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 801 GGTGACC G^GT[ACGT]ACC
EcoRI 271 GAATTC G^AATTC
Hin1I 96 GGCGCC G[GA]^CG[CT]C
HincII 2042 GTCAAC GT[CT]^[GA]AC
HindII 2042 GTCAAC GT[CT]^[GA]AC
KasI 96 GGCGCC G^GCGCC
KpnI 454 GGTACC GGTAC^C
NarI 96 GGCGCC GG^CGCC
NcoI 4072 CCATGG C^CATGG
NspI 586 GCATGC [GA]CATG^[CT]
PaeR7I 3840 CTCGAG C^TCGAG
PflFI 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4288 GAAGAGC GCTCTTC(1/4)
ScaI 2781 AGTACT AGT^ACT
SfoI 96 GGCGCC GGC^GCC
SpeI 1811 ACTAGT A^CTAGT
SphI 586 GCATGC GCATG^C
Tth111I 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3840 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 78 CGCG CG^CG
139 CGCG
AccIII 308 TCCGGA T^CCGGA
977 TCCGGA
AvrII 3190 CCTAGG C^CTAGG
4587 CCTAGG
BamHI 311 GGATCC G^GATCC
1104 GGATCC
BcgI 771 CGAGCCATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4633 GCAGCTGTATCG
BciVI 345 GGATAC GTATCC(6/5)
2258 GTATCC
BlnI 3190 CCTAGG C^CTAGG
4587 CCTAGG
BlpI 742 GCTGAGC GC^T[ACGT]AGC
4602 GCTGAGC
Bpu1102I 742 GCTGAGC GC^T[ACGT]AGC
4602 GCTGAGC
BseAI 308 TCCGGA T^CCGGA
977 TCCGGA
BspEI 308 TCCGGA T^CCGGA
977 TCCGGA
BssSI 3290 CTCGTG CACGAG(-5/-1)
4755 CTCGTG
BstAPI 232 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1955 GCACACCTTGC
BstUI 78 CGCG CG^CG
139 CGCG
CelII 742 GCTGAGC GC^T[ACGT]AGC
4602 GCTGAGC
DraIII 1854 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3703 CACTGCGTG
HindIII 2519 AAGCTT A^AGCTT
3495 AAGCTT
MfeI 1795 CAATTG C^AATTG
4179 CAATTG
MroI 308 TCCGGA T^CCGGA
977 TCCGGA
MunI 1795 CAATTG C^AATTG
4179 CAATTG
MvnI 78 CGCG CG^CG
139 CGCG
PpuMI 644 GGGTCCT [GA]G^G[AT]CC[CT]
4647 AGGTCCC
XbaI 1083 TCTAGA T^CTAGA
1937 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI