################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AclI AflIII AgeI Aor51HI AscI AseI AsnI AviII BbrPI BbvCI BclI BglI BsaBI BsiEI BsiWI BspHI BspLU11I BssHII BstBI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI HpaI KspI MamI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 454 GGTACC G^GTACC AcyI 96 GGCGCC G[GA]^CG[CT]C AflII 3895 CTTAAG C^TTAAG AhdI 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 879 GTGCAC G^TGCAC ApaI 3890 GGGCCC GGGCC^C ApaLI 879 GTGCAC G^TGCAC Asp718I 454 GGTACC G^GTACC AspEI 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 96 GGCGCC GGCGC^C BfrI 3895 CTTAAG C^TTAAG BglII 4212 AGATCT A^GATCT BsaAI 3925 TACGTG [CT]AC^GT[GA] BsaHI 96 GGCGCC G[GA]^CG[CT]C BsaI 3016 GAGACC GGTCTC(1/5) BsmBI 1193 GAGACG CGTCTC(1/5) BsmI 3670 GCATTC GAATGC(1/-1) BspDI 4559 ATCGAT AT^CGAT BsrBI 1099 GAGCGG CCGCTC(-3/-3) Bst1107I 1416 GTATAC GTA^TAC BstEII 801 GGTGACC G^GT[ACGT]ACC BstPI 801 GGTGACC G^GT[ACGT]ACC BstZ17I 1416 GTATAC GTA^TAC ClaI 4559 ATCGAT AT^CGAT DraI 3095 TTTAAA TTT^AAA DrdI 637 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 4072 CCATGG C^C[GA][CT]GG Eam1105I 3138 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 801 GGTGACC G^GT[ACGT]ACC EcoRI 271 GAATTC G^AATTC Hin1I 96 GGCGCC G[GA]^CG[CT]C HincII 2042 GTCAAC GT[CT]^[GA]AC HindII 2042 GTCAAC GT[CT]^[GA]AC KasI 96 GGCGCC G^GCGCC KpnI 454 GGTACC GGTAC^C NarI 96 GGCGCC GG^CGCC NcoI 4072 CCATGG C^CATGG NspI 586 GCATGC [GA]CATG^[CT] PaeR7I 3840 CTCGAG C^TCGAG PflFI 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 4288 GAAGAGC GCTCTTC(1/4) ScaI 2781 AGTACT AGT^ACT SfoI 96 GGCGCC GGC^GCC SpeI 1811 ACTAGT A^CTAGT SphI 586 GCATGC GCATG^C Tth111I 3993 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3840 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 78 CGCG CG^CG 139 CGCG AccIII 308 TCCGGA T^CCGGA 977 TCCGGA AvrII 3190 CCTAGG C^CTAGG 4587 CCTAGG BamHI 311 GGATCC G^GATCC 1104 GGATCC BcgI 771 CGAGCCATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4633 GCAGCTGTATCG BciVI 345 GGATAC GTATCC(6/5) 2258 GTATCC BlnI 3190 CCTAGG C^CTAGG 4587 CCTAGG BlpI 742 GCTGAGC GC^T[ACGT]AGC 4602 GCTGAGC Bpu1102I 742 GCTGAGC GC^T[ACGT]AGC 4602 GCTGAGC BseAI 308 TCCGGA T^CCGGA 977 TCCGGA BspEI 308 TCCGGA T^CCGGA 977 TCCGGA BssSI 3290 CTCGTG CACGAG(-5/-1) 4755 CTCGTG BstAPI 232 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1955 GCACACCTTGC BstUI 78 CGCG CG^CG 139 CGCG CelII 742 GCTGAGC GC^T[ACGT]AGC 4602 GCTGAGC DraIII 1854 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 3703 CACTGCGTG HindIII 2519 AAGCTT A^AGCTT 3495 AAGCTT MfeI 1795 CAATTG C^AATTG 4179 CAATTG MroI 308 TCCGGA T^CCGGA 977 TCCGGA MunI 1795 CAATTG C^AATTG 4179 CAATTG MvnI 78 CGCG CG^CG 139 CGCG PpuMI 644 GGGTCCT [GA]G^G[AT]CC[CT] 4647 AGGTCCC XbaI 1083 TCTAGA T^CTAGA 1937 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI