##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp718I BamHI BbeI BfrI BsaBI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BstBI ClaI CpoI DraIII
EcoRI EcoRV EcoT22I FseI HindIII
KasI KpnI MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I RcaI
RsrII SalI SfiI SfoI SfuI
SgrAI SnaBI SspI SwaI Tsp509I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2068 GTATAC GT^[AC][GT]AC
AccIII 757 TCCGGA T^CCGGA
AflIII 807 ACGTGT A^C[GA][CT]GT
AspI 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2918 GTATCC GTATCC(6/5)
BglI 3846 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2238 AGATCT A^GATCT
BseAI 757 TCCGGA T^CCGGA
Bsp1407I 5484 TGTACA T^GTACA
BspEI 757 TCCGGA T^CCGGA
BsrGI 5484 TGTACA T^GTACA
Bst1107I 2068 GTATAC GTA^TAC
BstEII 856 GGTGACC G^GT[ACGT]ACC
BstPI 856 GGTGACC G^GT[ACGT]ACC
BstZ17I 2068 GTATAC GTA^TAC
DraI 5399 TTTAAA TTT^AAA
DrdI 3140 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4599 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 856 GGTGACC G^GT[ACGT]ACC
KspI 3700 CCGCGG CCGC^GG
MroI 757 TCCGGA T^CCGGA
PflFI 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2852 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2544 CGATCG CGAT^CG
SacII 3700 CCGCGG CCGC^GG
SapI 1862 GAAGAGC GCTCTTC(1/4)
SbfI 814 CCTGCAGG CCTGCA^GG
ScaI 4422 AGTACT AGT^ACT
SexAI 4372 ACCTGGT A^CC[AT]GGT
SmaI 3640 CCCGGG CCC^GGG
SpeI 5472 ACTAGT A^CTAGT
Sse8387I 814 CCTGCAGG CCTGCA^GG
SspBI 5484 TGTACA T^GTACA
TfiI 4132 GAATC G^A[AT]TC
Tth111I 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3640 CCCGGG C^CCGGG
XmaI 3640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 2099 GTGCAC G^TGCAC
4887 GTGCAC
Aor51HI 162 AGCGCT AGC^GCT
1027 AGCGCT
ApaLI 2099 GTGCAC G^TGCAC
4887 GTGCAC
Asp700I 2227 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGCACTTC
AviII 2952 TGCGCA TGC^GCA
3885 TGCGCA
BbrPI 434 CACGTG CAC^GTG
1478 CACGTG
BclI 3421 TGATCA T^GATCA
4621 TGATCA
BsaAI 434 CACGTG [CT]AC^GT[GA]
1478 CACGTG
BsaI 4118 GAGACC GGTCTC(1/5)
5405 GAGACC
BsrBI 298 CCGCTC CCGCTC(-3/-3)
413 CCGCTC
BssHII 291 GCGCGC G^CGCGC
293 GCGCGC
EagI 1788 CGGCCG C^GGCCG
5273 CGGCCG
EclXI 1788 CGGCCG C^GGCCG
5273 CGGCCG
Eco47III 162 AGCGCT AGC^GCT
1027 AGCGCT
Eco52I 1788 CGGCCG C^GGCCG
5273 CGGCCG
FbaI 3421 TGATCA T^GATCA
4621 TGATCA
FspI 2952 TGCGCA TGC^GCA
3885 TGCGCA
HincII 1297 GTTAAC GT[CT]^[GA]AC
2533 GTTAAC
HindII 1297 GTTAAC GT[CT]^[GA]AC
2533 GTTAAC
HpaI 1297 GTTAAC GTT^AAC
2533 GTTAAC
NaeI 2014 GCCGGC GCC^GGC
2187 GCCGGC
NgoMIV 2014 GCCGGC G^CCGGC
2187 GCCGGC
NspI 3645 GCATGC [GA]CATG^[CT]
4758 GCATGC
PflMI 1312 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4367 CCAACACCTGG
PmaCI 434 CACGTG CAC^GTG
1478 CACGTG
PmlI 434 CACGTG CAC^GTG
1478 CACGTG
SacI 1835 GAGCTC GAGCT^C
4836 GAGCTC
SphI 3645 GCATGC GCATG^C
4758 GCATGC
StuI 1878 AGGCCT AGG^CCT
3994 AGGCCT
Van91I 1312 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4367 CCAACACCTGG
XmnI 2227 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PaeR7I PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I XbaI XcmI XhoI XhoII