################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp718I BamHI BbeI BfrI BsaBI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BstBI ClaI CpoI DraIII EcoRI EcoRV EcoT22I FseI HindIII KasI KpnI MamI MfeI MluI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SspI SwaI Tsp509I ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 2068 GTATAC GT^[AC][GT]AC AccIII 757 TCCGGA T^CCGGA AflIII 807 ACGTGT A^C[GA][CT]GT AspI 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 2918 GTATCC GTATCC(6/5) BglI 3846 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2238 AGATCT A^GATCT BseAI 757 TCCGGA T^CCGGA Bsp1407I 5484 TGTACA T^GTACA BspEI 757 TCCGGA T^CCGGA BsrGI 5484 TGTACA T^GTACA Bst1107I 2068 GTATAC GTA^TAC BstEII 856 GGTGACC G^GT[ACGT]ACC BstPI 856 GGTGACC G^GT[ACGT]ACC BstZ17I 2068 GTATAC GTA^TAC DraI 5399 TTTAAA TTT^AAA DrdI 3140 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4599 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 856 GGTGACC G^GT[ACGT]ACC KspI 3700 CCGCGG CCGC^GG MroI 757 TCCGGA T^CCGGA PflFI 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2852 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 2544 CGATCG CGAT^CG SacII 3700 CCGCGG CCGC^GG SapI 1862 GAAGAGC GCTCTTC(1/4) SbfI 814 CCTGCAGG CCTGCA^GG ScaI 4422 AGTACT AGT^ACT SexAI 4372 ACCTGGT A^CC[AT]GGT SmaI 3640 CCCGGG CCC^GGG SpeI 5472 ACTAGT A^CTAGT Sse8387I 814 CCTGCAGG CCTGCA^GG SspBI 5484 TGTACA T^GTACA TfiI 4132 GAATC G^A[AT]TC Tth111I 3092 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 3640 CCCGGG C^CCGGG XmaI 3640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Alw44I 2099 GTGCAC G^TGCAC 4887 GTGCAC Aor51HI 162 AGCGCT AGC^GCT 1027 AGCGCT ApaLI 2099 GTGCAC G^TGCAC 4887 GTGCAC Asp700I 2227 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2747 GAAGCACTTC AviII 2952 TGCGCA TGC^GCA 3885 TGCGCA BbrPI 434 CACGTG CAC^GTG 1478 CACGTG BclI 3421 TGATCA T^GATCA 4621 TGATCA BsaAI 434 CACGTG [CT]AC^GT[GA] 1478 CACGTG BsaI 4118 GAGACC GGTCTC(1/5) 5405 GAGACC BsrBI 298 CCGCTC CCGCTC(-3/-3) 413 CCGCTC BssHII 291 GCGCGC G^CGCGC 293 GCGCGC EagI 1788 CGGCCG C^GGCCG 5273 CGGCCG EclXI 1788 CGGCCG C^GGCCG 5273 CGGCCG Eco47III 162 AGCGCT AGC^GCT 1027 AGCGCT Eco52I 1788 CGGCCG C^GGCCG 5273 CGGCCG FbaI 3421 TGATCA T^GATCA 4621 TGATCA FspI 2952 TGCGCA TGC^GCA 3885 TGCGCA HincII 1297 GTTAAC GT[CT]^[GA]AC 2533 GTTAAC HindII 1297 GTTAAC GT[CT]^[GA]AC 2533 GTTAAC HpaI 1297 GTTAAC GTT^AAC 2533 GTTAAC NaeI 2014 GCCGGC GCC^GGC 2187 GCCGGC NgoMIV 2014 GCCGGC G^CCGGC 2187 GCCGGC NspI 3645 GCATGC [GA]CATG^[CT] 4758 GCATGC PflMI 1312 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4367 CCAACACCTGG PmaCI 434 CACGTG CAC^GTG 1478 CACGTG PmlI 434 CACGTG CAC^GTG 1478 CACGTG SacI 1835 GAGCTC GAGCT^C 4836 GAGCTC SphI 3645 GCATGC GCATG^C 4758 GCATGC StuI 1878 AGGCCT AGG^CCT 3994 AGGCCT Van91I 1312 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4367 CCAACACCTGG XmnI 2227 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2747 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XbaI XcmI XhoI XhoII