##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI Asp718I AspEI AviII
BamHI BciVI BclI BfrI BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
Eam1105I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SexAI SfuI SgrAI
SnaBI SpeI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 3807 TCCGGA T^CCGGA
AcsI 3171 AAATTC [GA]^AATT[CT]
AflIII 475 ACATGT A^C[GA][CT]GT
Alw44I 4983 GTGCAC G^TGCAC
ApaLI 4983 GTGCAC G^TGCAC
ApoI 3171 AAATTC [GA]^AATT[CT]
AseI 4557 ATTAAT AT^TAAT
AsnI 4557 ATTAAT AT^TAAT
Asp700I 666 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 2941 GGCGCC GGCGC^C
BbrPI 4057 CACGTG CAC^GTG
BglII 1084 AGATCT A^GATCT
BsaBI 1511 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3803 GGTCTC GGTCTC(1/5)
BseAI 3807 TCCGGA T^CCGGA
Bsp1407I 743 TGTACA T^GTACA
BspEI 3807 TCCGGA T^CCGGA
BspLU11I 475 ACATGT A^CATGT
BsrBI 5392 CCGCTC CCGCTC(-3/-3)
BsrGI 743 TGTACA T^GTACA
DraI 4628 TTTAAA TTT^AAA
DrdI 2140 GACCTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1485 GATATC GAT^ATC
HpaI 2544 GTTAAC GTT^AAC
KasI 2941 GGCGCC G^GCGCC
KspI 39 CCGCGG CCGC^GG
MamI 1511 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3807 TCCGGA T^CCGGA
NarI 2941 GGCGCC GG^CGCC
PmaCI 4057 CACGTG CAC^GTG
PmlI 4057 CACGTG CAC^GTG
PshAI 3622 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4557 ATTAAT AT^TAAT
SacII 39 CCGCGG CCGC^GG
SbfI 1502 CCTGCAGG CCTGCA^GG
ScaI 1158 AGTACT AGT^ACT
SfoI 2941 GGCGCC GGC^GCC
Sse8387I 1502 CCTGCAGG CCTGCA^GG
SspBI 743 TGTACA T^GTACA
SspI 4641 AATATT AAT^ATT
XmnI 666 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 186 GTAGAC GT^[AC][GT]AC
393 GTAGAC
Aor51HI 3683 AGCGCT AGC^GCT
3849 AGCGCT
AspI 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3182 GACAGAGTC
AvrII 4354 CCTAGG C^CTAGG
5042 CCTAGG
BbsI 3987 GAAGAC GAAGAC(2/6)
2786 GTCTTC
BlnI 4354 CCTAGG C^CTAGG
5042 CCTAGG
BpuAI 3987 GAAGAC GAAGAC(2/6)
2786 GTCTTC
BsaWI 943 ACCGGA [AT]^CCGG[AT]
3807 TCCGGA
BsmBI 3431 CGTCTC CGTCTC(1/5)
5171 CGTCTC
BspHI 638 TCATGA T^CATGA
935 TCATGA
BssSI 4771 CACGAG CACGAG(-5/-1)
5217 CTCGTG
BstYI 1084 AGATCT [GA]^GATC[CT]
3469 AGATCC
Eco47III 3683 AGCGCT AGC^GCT
3849 AGCGCT
HincII 87 GTCAAC GT[CT]^[GA]AC
2544 GTTAAC
HindII 87 GTCAAC GT[CT]^[GA]AC
2544 GTTAAC
MflI 1084 AGATCT [GA]^GATC[CT]
3469 AGATCC
PflFI 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3182 GACAGAGTC
RcaI 638 TCATGA T^CATGA
935 TCATGA
SfiI 1900 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4392 GGCCACTGTGGCC
SphI 1178 GCATGC GCATG^C
5033 GCATGC
Tth111I 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3182 GACAGAGTC
XhoII 1084 AGATCT [GA]^GATC[CT]
3469 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI