################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AscI Asp718I AspEI AviII BamHI BciVI BclI BfrI BsiWI BsmI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacI SalI SexAI SfuI SgrAI SnaBI SpeI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 3807 TCCGGA T^CCGGA AcsI 3171 AAATTC [GA]^AATT[CT] AflIII 475 ACATGT A^C[GA][CT]GT Alw44I 4983 GTGCAC G^TGCAC ApaLI 4983 GTGCAC G^TGCAC ApoI 3171 AAATTC [GA]^AATT[CT] AseI 4557 ATTAAT AT^TAAT AsnI 4557 ATTAAT AT^TAAT Asp700I 666 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 2941 GGCGCC GGCGC^C BbrPI 4057 CACGTG CAC^GTG BglII 1084 AGATCT A^GATCT BsaBI 1511 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3803 GGTCTC GGTCTC(1/5) BseAI 3807 TCCGGA T^CCGGA Bsp1407I 743 TGTACA T^GTACA BspEI 3807 TCCGGA T^CCGGA BspLU11I 475 ACATGT A^CATGT BsrBI 5392 CCGCTC CCGCTC(-3/-3) BsrGI 743 TGTACA T^GTACA DraI 4628 TTTAAA TTT^AAA DrdI 2140 GACCTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1485 GATATC GAT^ATC HpaI 2544 GTTAAC GTT^AAC KasI 2941 GGCGCC G^GCGCC KspI 39 CCGCGG CCGC^GG MamI 1511 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3807 TCCGGA T^CCGGA NarI 2941 GGCGCC GG^CGCC PmaCI 4057 CACGTG CAC^GTG PmlI 4057 CACGTG CAC^GTG PshAI 3622 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4557 ATTAAT AT^TAAT SacII 39 CCGCGG CCGC^GG SbfI 1502 CCTGCAGG CCTGCA^GG ScaI 1158 AGTACT AGT^ACT SfoI 2941 GGCGCC GGC^GCC Sse8387I 1502 CCTGCAGG CCTGCA^GG SspBI 743 TGTACA T^GTACA SspI 4641 AATATT AAT^ATT XmnI 666 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 186 GTAGAC GT^[AC][GT]AC 393 GTAGAC Aor51HI 3683 AGCGCT AGC^GCT 3849 AGCGCT AspI 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3182 GACAGAGTC AvrII 4354 CCTAGG C^CTAGG 5042 CCTAGG BbsI 3987 GAAGAC GAAGAC(2/6) 2786 GTCTTC BlnI 4354 CCTAGG C^CTAGG 5042 CCTAGG BpuAI 3987 GAAGAC GAAGAC(2/6) 2786 GTCTTC BsaWI 943 ACCGGA [AT]^CCGG[AT] 3807 TCCGGA BsmBI 3431 CGTCTC CGTCTC(1/5) 5171 CGTCTC BspHI 638 TCATGA T^CATGA 935 TCATGA BssSI 4771 CACGAG CACGAG(-5/-1) 5217 CTCGTG BstYI 1084 AGATCT [GA]^GATC[CT] 3469 AGATCC Eco47III 3683 AGCGCT AGC^GCT 3849 AGCGCT HincII 87 GTCAAC GT[CT]^[GA]AC 2544 GTTAAC HindII 87 GTCAAC GT[CT]^[GA]AC 2544 GTTAAC MflI 1084 AGATCT [GA]^GATC[CT] 3469 AGATCC PflFI 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3182 GACAGAGTC RcaI 638 TCATGA T^CATGA 935 TCATGA SfiI 1900 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4392 GGCCACTGTGGCC SphI 1178 GCATGC GCATG^C 5033 GCATGC Tth111I 617 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3182 GACAGAGTC XhoII 1084 AGATCT [GA]^GATC[CT] 3469 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI