################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## Acc65I AccIII AgeI AhdI AscI Asp718I AspEI AviII BcgI BglI BglII BlpI Bpu1102I BseAI BsiEI BsiWI BspDI BspEI BspLU11I CelII ClaI CpoI EagI Eam1105I EclXI Eco52I FseI FspI KpnI KspI MfeI MluI MroI MunI NheI NotI PacI PinAI PmeI PshAI PvuI RsrII SacII SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 2809 GACGTC GACGT^C AclI 1258 AACGTT AA^CGTT AflII 322 CTTAAG C^TTAAG AocI 281 CCTGAGG CC^T[ACGT]AGG ApaI 3232 GGGCCC GGGCC^C AseI 4192 ATTAAT AT^TAAT AsnI 4192 ATTAAT AT^TAAT AvrII 4657 CCTAGG C^CTAGG BamHI 4315 GGATCC G^GATCC BbrPI 2296 CACGTG CAC^GTG BbvCI 4408 CCTCAGC CCTCAGC(-5/-2) BciVI 2831 GTATCC GTATCC(6/5) BclI 549 TGATCA T^GATCA BfrI 322 CTTAAG C^TTAAG BlnI 4657 CCTAGG C^CTAGG BsaBI 2663 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 850 CGTCTC CGTCTC(1/5) BspMI 4758 ACCTGC ACCTGC(4/8) BsrBI 1028 GAGCGG CCGCTC(-3/-3) BsrFI 3594 GCCGGC [GA]^CCGG[CT] BssHII 555 GCGCGC G^CGCGC Bst1107I 2020 GTATAC GTA^TAC BstAPI 466 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4968 TTCGAA TT^CGAA BstZ17I 2020 GTATAC GTA^TAC Bsu36I 281 CCTGAGG CC^T[ACGT]AGG Cfr10I 3594 GCCGGC [GA]^CCGG[CT] DrdI 728 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 281 CCTGAGG CC^T[ACGT]AGG EcoRI 4429 GAATTC G^AATTC EcoRV 3605 GATATC GAT^ATC FbaI 549 TGATCA T^GATCA HgaI 2982 GCGTC GACGC(5/10) HpaI 1636 GTTAAC GTT^AAC MamI 2663 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3594 GCCGGC GCC^GGC NcoI 2673 CCATGG C^CATGG NdeI 4112 CATATG CA^TATG NgoMIV 3594 GCCGGC G^CCGGC NruI 3609 TCGCGA TCG^CGA NspV 4968 TTCGAA TT^CGAA PaeR7I 5339 CTCGAG C^TCGAG PmaCI 2296 CACGTG CAC^GTG PmlI 2296 CACGTG CAC^GTG PshBI 4192 ATTAAT AT^TAAT Psp1406I 1258 AACGTT AA^CGTT SacI 3091 GAGCTC GAGCT^C SexAI 1845 ACCAGGT A^CC[AT]GGT SfuI 4968 TTCGAA TT^CGAA SmaI 1573 CCCGGG CCC^GGG SwaI 3474 ATTTAAAT ATTT^AAAT XbaI 4469 TCTAGA T^CTAGA XhoI 5339 CTCGAG C^TCGAG XmaCI 1573 CCCGGG C^CCGGG XmaI 1573 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 812 GTCTAC GT^[AC][GT]AC 2020 GTATAC Alw44I 820 GTGCAC G^TGCAC 3014 GTGCAC AlwNI 3741 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 5282 CAGAACCTG Aor51HI 1937 AGCGCT AGC^GCT 2336 AGCGCT ApaLI 820 GTGCAC G^TGCAC 3014 GTGCAC AspI 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4512 GACTATGTC BalI 2823 TGGCCA TGG^CCA 3937 TGGCCA BsaAI 2296 CACGTG [CT]AC^GT[GA] 5582 TACGTG BseRI 3094 CTCCTC GAGGAG(10/8) 3653 CTCCTC Bsp1407I 4562 TGTACA T^GTACA 5060 TGTACA BspHI 777 TCATGA T^CATGA 1945 TCATGA BsrGI 4562 TGTACA T^GTACA 5060 TGTACA BssSI 1562 CTCGTG CACGAG(-5/-1) 4507 CTCGTG BstEII 736 GGTCACC G^GT[ACGT]ACC 882 GGTCACC BstPI 736 GGTCACC G^GT[ACGT]ACC 882 GGTCACC DraIII 1020 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4850 CACGCAGTG DsaI 1992 CCGTGG C^C[GA][CT]GG 2673 CCATGG Eco47III 1937 AGCGCT AGC^GCT 2336 AGCGCT EcoNI 749 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1075 CCTCCCAGAGG EcoO65I 736 GGTCACC G^GT[ACGT]ACC 882 GGTCACC MluNI 2823 TGGCCA TGG^CCA 3937 TGGCCA MscI 2823 TGGCCA TGG^CCA 3937 TGGCCA PflFI 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4512 GACTATGTC PflMI 1908 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2187 CCATTATGTGG RcaI 777 TCATGA T^CATGA 1945 TCATGA SapI 295 GAAGAGC GCTCTTC(1/4) 1940 GCTCTTC SphI 3162 GCATGC GCATG^C 5186 GCATGC SspBI 4562 TGTACA T^GTACA 5060 TGTACA SspI 641 AATATT AAT^ATT 985 AATATT Tth111I 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4512 GACTATGTC Van91I 1908 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2187 CCATTATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI