##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AscI
Asp718I AspEI AviII BcgI BglI
BglII BlpI Bpu1102I BseAI BsiEI
BsiWI BspDI BspEI BspLU11I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco52I FseI FspI KpnI KspI
MfeI MluI MroI MunI NheI
NotI PacI PinAI PmeI PshAI
PvuI RsrII SacII SalI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2809 GACGTC GACGT^C
AclI 1258 AACGTT AA^CGTT
AflII 322 CTTAAG C^TTAAG
AocI 281 CCTGAGG CC^T[ACGT]AGG
ApaI 3232 GGGCCC GGGCC^C
AseI 4192 ATTAAT AT^TAAT
AsnI 4192 ATTAAT AT^TAAT
AvrII 4657 CCTAGG C^CTAGG
BamHI 4315 GGATCC G^GATCC
BbrPI 2296 CACGTG CAC^GTG
BbvCI 4408 CCTCAGC CCTCAGC(-5/-2)
BciVI 2831 GTATCC GTATCC(6/5)
BclI 549 TGATCA T^GATCA
BfrI 322 CTTAAG C^TTAAG
BlnI 4657 CCTAGG C^CTAGG
BsaBI 2663 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 850 CGTCTC CGTCTC(1/5)
BspMI 4758 ACCTGC ACCTGC(4/8)
BsrBI 1028 GAGCGG CCGCTC(-3/-3)
BsrFI 3594 GCCGGC [GA]^CCGG[CT]
BssHII 555 GCGCGC G^CGCGC
Bst1107I 2020 GTATAC GTA^TAC
BstAPI 466 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4968 TTCGAA TT^CGAA
BstZ17I 2020 GTATAC GTA^TAC
Bsu36I 281 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3594 GCCGGC [GA]^CCGG[CT]
DrdI 728 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 281 CCTGAGG CC^T[ACGT]AGG
EcoRI 4429 GAATTC G^AATTC
EcoRV 3605 GATATC GAT^ATC
FbaI 549 TGATCA T^GATCA
HgaI 2982 GCGTC GACGC(5/10)
HpaI 1636 GTTAAC GTT^AAC
MamI 2663 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3594 GCCGGC GCC^GGC
NcoI 2673 CCATGG C^CATGG
NdeI 4112 CATATG CA^TATG
NgoMIV 3594 GCCGGC G^CCGGC
NruI 3609 TCGCGA TCG^CGA
NspV 4968 TTCGAA TT^CGAA
PaeR7I 5339 CTCGAG C^TCGAG
PmaCI 2296 CACGTG CAC^GTG
PmlI 2296 CACGTG CAC^GTG
PshBI 4192 ATTAAT AT^TAAT
Psp1406I 1258 AACGTT AA^CGTT
SacI 3091 GAGCTC GAGCT^C
SexAI 1845 ACCAGGT A^CC[AT]GGT
SfuI 4968 TTCGAA TT^CGAA
SmaI 1573 CCCGGG CCC^GGG
SwaI 3474 ATTTAAAT ATTT^AAAT
XbaI 4469 TCTAGA T^CTAGA
XhoI 5339 CTCGAG C^TCGAG
XmaCI 1573 CCCGGG C^CCGGG
XmaI 1573 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 812 GTCTAC GT^[AC][GT]AC
2020 GTATAC
Alw44I 820 GTGCAC G^TGCAC
3014 GTGCAC
AlwNI 3741 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
5282 CAGAACCTG
Aor51HI 1937 AGCGCT AGC^GCT
2336 AGCGCT
ApaLI 820 GTGCAC G^TGCAC
3014 GTGCAC
AspI 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACTATGTC
BalI 2823 TGGCCA TGG^CCA
3937 TGGCCA
BsaAI 2296 CACGTG [CT]AC^GT[GA]
5582 TACGTG
BseRI 3094 CTCCTC GAGGAG(10/8)
3653 CTCCTC
Bsp1407I 4562 TGTACA T^GTACA
5060 TGTACA
BspHI 777 TCATGA T^CATGA
1945 TCATGA
BsrGI 4562 TGTACA T^GTACA
5060 TGTACA
BssSI 1562 CTCGTG CACGAG(-5/-1)
4507 CTCGTG
BstEII 736 GGTCACC G^GT[ACGT]ACC
882 GGTCACC
BstPI 736 GGTCACC G^GT[ACGT]ACC
882 GGTCACC
DraIII 1020 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4850 CACGCAGTG
DsaI 1992 CCGTGG C^C[GA][CT]GG
2673 CCATGG
Eco47III 1937 AGCGCT AGC^GCT
2336 AGCGCT
EcoNI 749 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1075 CCTCCCAGAGG
EcoO65I 736 GGTCACC G^GT[ACGT]ACC
882 GGTCACC
MluNI 2823 TGGCCA TGG^CCA
3937 TGGCCA
MscI 2823 TGGCCA TGG^CCA
3937 TGGCCA
PflFI 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACTATGTC
PflMI 1908 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2187 CCATTATGTGG
RcaI 777 TCATGA T^CATGA
1945 TCATGA
SapI 295 GAAGAGC GCTCTTC(1/4)
1940 GCTCTTC
SphI 3162 GCATGC GCATG^C
5186 GCATGC
SspBI 4562 TGTACA T^GTACA
5060 TGTACA
SspI 641 AATATT AAT^ATT
985 AATATT
Tth111I 4174 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4512 GACTATGTC
Van91I 1908 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2187 CCATTATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI