##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I ApaLI AseI AsnI
AspEI AviII BbeI BciVI BfrI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I FspI HindIII
HpaI KasI MluI MroI NarI
NdeI NheI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 296 GACGCC G[GA]^CG[CT]C
AgeI 3603 ACCGGT A^CCGGT
AscI 13 GGCGCGCC GG^CGCGCC
AvrII 2015 CCTAGG C^CTAGG
BamHI 1118 GGATCC G^GATCC
BbrPI 3405 CACGTG CAC^GTG
BbvCI 1423 GCTGAGG CCTCAGC(-5/-2)
BcgI 4175 CGAAAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2015 CCTAGG C^CTAGG
BsaAI 3405 CACGTG [CT]AC^GT[GA]
BsaBI 3451 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 296 GACGCC G[GA]^CG[CT]C
BsiEI 4613 CGGTCG CG[GA][CT]^CG
BsmI 4949 GCATTC GAATGC(1/-1)
BspHI 2473 TCATGA T^CATGA
BsrBI 2556 CCGCTC CCGCTC(-3/-3)
BssHII 14 GCGCGC G^CGCGC
BssSI 458 CTCGTG CACGAG(-5/-1)
BstEII 4938 GGTAACC G^GT[ACGT]ACC
BstPI 4938 GGTAACC G^GT[ACGT]ACC
EcoO65I 4938 GGTAACC G^GT[ACGT]ACC
EcoRI 2242 GAATTC G^AATTC
EcoRV 2306 GATATC GAT^ATC
EcoT22I 929 ATGCAT ATGCA^T
FseI 3081 GGCCGGCC GGCCGG^CC
Hin1I 296 GACGCC G[GA]^CG[CT]C
KspI 150 CCGCGG CCGC^GG
MamI 3451 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 540 CAATTG C^AATTG
MunI 540 CAATTG C^AATTG
NaeI 3082 GCCGGC GCC^GGC
NgoMIV 3082 GCCGGC G^CCGGC
NsiI 929 ATGCAT ATGCA^T
NspI 830 GCATGC [GA]CATG^[CT]
PflMI 2255 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3603 ACCGGT A^CCGGT
PmaCI 3405 CACGTG CAC^GTG
PmlI 3405 CACGTG CAC^GTG
RcaI 2473 TCATGA T^CATGA
SacII 150 CCGCGG CCGC^GG
SexAI 1783 ACCTGGT A^CC[AT]GGT
SfiI 4220 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 61 CCCGGG CCC^GGG
SphI 830 GCATGC GCATG^C
Van91I 2255 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2810 GGTACC G^GTACC
2848 GGTACC
Aor51HI 2150 AGCGCT AGC^GCT
3338 AGCGCT
Asp700I 1505 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4392 GAAGGTGTTC
Asp718I 2810 GGTACC G^GTACC
2848 GGTACC
BclI 1789 TGATCA T^GATCA
4021 TGATCA
BglII 393 AGATCT A^GATCT
3469 AGATCT
BlpI 3128 GCTCAGC GC^T[ACGT]AGC
3972 GCTCAGC
Bpu1102I 3128 GCTCAGC GC^T[ACGT]AGC
3972 GCTCAGC
BsaWI 3603 ACCGGT [AT]^CCGG[AT]
4485 ACCGGA
BsrDI 1773 GCAATG GCAATG(2/0)
2835 GCAATG
CelII 3128 GCTCAGC GC^T[ACGT]AGC
3972 GCTCAGC
DraIII 1782 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3161 CACAAAGTG
DrdI 1623 GACAGGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3848 GACTATTGTGTC
Eco47III 2150 AGCGCT AGC^GCT
3338 AGCGCT
FbaI 1789 TGATCA T^GATCA
4021 TGATCA
KpnI 2810 GGTACC GGTAC^C
2848 GGTACC
MslI 3987 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4670 CATTTTCGTG
SacI 475 GAGCTC GAGCT^C
1252 GAGCTC
SapI 1505 GAAGAGC GCTCTTC(1/4)
2982 GAAGAGC
XmnI 1505 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4392 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PaeR7I
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoI XhoII