##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
Aor51HI ApaI AscI Asp718I AspI
AviII AvrII BamHI BbeI BcgI
BglII BlnI BsaHI BsaI BseAI
BsiWI BspEI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DraIII DrdI Eco47III EcoNI EcoO65I
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NspV PflFI PmeI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3221 GTCTAC GT^[AC][GT]AC
AccII 1042 CGCG CG^CG
AgeI 3258 ACCGGT A^CCGGT
AhdI 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2656 CACGTG CAC^GTG
BbsI 3287 GTCTTC GAAGAC(2/6)
BbvCI 1221 CCTCAGC CCTCAGC(-5/-2)
BglI 3054 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3287 GTCTTC GAAGAC(2/6)
BsaAI 2656 CACGTG [CT]AC^GT[GA]
BsaWI 3258 ACCGGT [AT]^CCGG[AT]
BsiEI 2863 CGGCCG CG[GA][CT]^CG
BsmBI 2100 GAGACG CGTCTC(1/5)
BsmI 413 GCATTC GAATGC(1/-1)
Bsp1407I 1765 TGTACA T^GTACA
BsrDI 5431 CATTGC GCAATG(2/0)
BsrGI 1765 TGTACA T^GTACA
BssSI 1024 CACGAG CACGAG(-5/-1)
BstUI 1042 CGCG CG^CG
DraII 2700 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1966 CCACGG C^C[GA][CT]GG
EagI 2863 CGGCCG C^GGCCG
Eam1105I 3279 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2863 CGGCCG C^GGCCG
Eco52I 2863 CGGCCG C^GGCCG
EcoO109I 2700 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2228 GAATTC G^AATTC
EcoRV 5069 GATATC GAT^ATC
EcoT22I 160 ATGCAT ATGCA^T
MvnI 1042 CGCG CG^CG
NotI 2862 GCGGCCGC GC^GGCCGC
NsiI 160 ATGCAT ATGCA^T
PflMI 1421 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3258 ACCGGT A^CCGGT
PmaCI 2656 CACGTG CAC^GTG
PmlI 2656 CACGTG CAC^GTG
PpuMI 2700 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 639 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SpeI 310 ACTAGT A^CTAGT
SspBI 1765 TGTACA T^GTACA
StuI 2361 AGGCCT AGG^CCT
Van91I 1421 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 239 CTTAAG C^TTAAG
2393 CTTAAG
Alw44I 2659 GTGCAC G^TGCAC
3312 GTGCAC
AocI 2504 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
ApaLI 2659 GTGCAC G^TGCAC
3312 GTGCAC
BanII 95 GAGCTC G[GA]GC[CT]^C
3299 GAGCTC
BciVI 389 GTATCC GTATCC(6/5)
3761 GTATCC
BcnI 1263 CCCGG CC^[GC]GG
1858 CCCGG
BfrI 239 CTTAAG C^TTAAG
2393 CTTAAG
BlpI 1527 GCTTAGC GC^T[ACGT]AGC
3342 GCTCAGC
Bpu1102I 1527 GCTTAGC GC^T[ACGT]AGC
3342 GCTCAGC
BsaBI 1356 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3486 GATGCTGATC
BspDI 3493 ATCGAT AT^CGAT
4174 ATCGAT
BspHI 3016 TCATGA T^CATGA
5416 TCATGA
BspLU11I 192 ACATGT A^CATGT
1949 ACATGT
BsrBI 3340 CCGCTC CCGCTC(-3/-3)
2820 GAGCGG
BsrFI 2178 GCCGGT [GA]^CCGG[CT]
3258 ACCGGT
Bsu36I 2504 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
CelII 1527 GCTTAGC GC^T[ACGT]AGC
3342 GCTCAGC
CfoI 1041 GCGC GCG^C
2915 GCGC
Cfr10I 2178 GCCGGT [GA]^CCGG[CT]
3258 ACCGGT
ClaI 3493 ATCGAT AT^CGAT
4174 ATCGAT
EarI 1091 CTCTTC CTCTTC(1/4)
1474 GAAGAG
Eco81I 2504 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
EcoT14I 1999 CCAAGG C^C[AT][AT]GG
3355 CCTTGG
HhaI 1041 GCGC GCG^C
2915 GCGC
HinP1I 1041 GCGC G^CGC
2915 GCGC
HindIII 3246 AAGCTT A^AGCTT
3936 AAGCTT
Ksp632I 1091 CTCTTC CTCTTC(1/4)
1474 GAAGAG
MamI 1356 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3486 GATGCTGATC
NciI 1263 CCCGG CC^[GC]GG
1858 CCCGG
PacI 924 TTAATTAA TTAAT^TAA
2074 TTAATTAA
RcaI 3016 TCATGA T^CATGA
5416 TCATGA
SacI 95 GAGCTC GAGCT^C
3299 GAGCTC
SphI 399 GCATGC GCATG^C
3775 GCATGC
StyI 1999 CCAAGG C^C[AT][AT]GG
3355 CCTTGG
XcmI 984 CCAGTTCTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3361 CCATGCTGATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanI BbvI BclI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EcoRII
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoI XhoII XmnI