##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AseI AsnI AspI AviII
BbeI BcgI BfrI BglII BsaBI
BsiEI BspDI BspHI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HindIII
KasI KspI MamI MluI NarI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2271 GGTACC G^GTACC
AccI 4056 GTCTAC GT^[AC][GT]AC
AccII 1957 CGCG CG^CG
AccIII 6 TCCGGA T^CCGGA
AcyI 333 GACGCC G[GA]^CG[CT]C
ApaI 3147 GGGCCC GGGCC^C
Asp700I 4572 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2271 GGTACC G^GTACC
BalI 27 TGGCCA TGG^CCA
BamHI 1275 GGATCC G^GATCC
BbrPI 3655 CACGTG CAC^GTG
BciVI 2429 GGATAC GTATCC(6/5)
BclI 4766 TGATCA T^GATCA
BglI 1998 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3655 CACGTG [CT]AC^GT[GA]
BsaHI 333 GACGCC G[GA]^CG[CT]C
BsaWI 6 TCCGGA [AT]^CCGG[AT]
BseAI 6 TCCGGA T^CCGGA
BsiWI 1482 CGTACG C^GTACG
BsmBI 855 CGTCTC CGTCTC(1/5)
Bsp1407I 5021 TGTACA T^GTACA
BspEI 6 TCCGGA T^CCGGA
BspLU11I 3716 ACATGT A^CATGT
BsrDI 4252 GCAATG GCAATG(2/0)
BsrFI 2223 GCCGGC [GA]^CCGG[CT]
BsrGI 5021 TGTACA T^GTACA
BssSI 1869 CTCGTG CACGAG(-5/-1)
BstUI 1957 CGCG CG^CG
Cfr10I 2223 GCCGGC [GA]^CCGG[CT]
EcoNI 1303 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4504 ATGCAT ATGCA^T
FbaI 4766 TGATCA T^GATCA
Hin1I 333 GACGCC G[GA]^CG[CT]C
HpaI 3372 GTTAAC GTT^AAC
KpnI 2271 GGTACC GGTAC^C
MfeI 3584 CAATTG C^AATTG
MluNI 27 TGGCCA TGG^CCA
MroI 6 TCCGGA T^CCGGA
MscI 27 TGGCCA TGG^CCA
MunI 3584 CAATTG C^AATTG
MvnI 1957 CGCG CG^CG
NaeI 2223 GCCGGC GCC^GGC
NgoMIV 2223 GCCGGC G^CCGGC
NheI 4217 GCTAGC G^CTAGC
NsiI 4504 ATGCAT ATGCA^T
PmaCI 3655 CACGTG CAC^GTG
PmlI 3655 CACGTG CAC^GTG
PshAI 2754 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 259 GCTCTTC GCTCTTC(1/4)
ScaI 4313 AGTACT AGT^ACT
SspBI 5021 TGTACA T^GTACA
SwaI 5038 ATTTAAAT ATTT^AAAT
XbaI 5111 TCTAGA T^CTAGA
XcmI 2093 CCAGAAGGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4572 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 2437 ACGTGT A^C[GA][CT]GT
3716 ACATGT
AhdI 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3978 GACTTCATGTC
Alw44I 3652 GTGCAC G^TGCAC
3658 GTGCAC
AocI 47 CCTGAGG CC^T[ACGT]AGG
3528 CCTCAGG
ApaLI 3652 GTGCAC G^TGCAC
3658 GTGCAC
AspEI 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3978 GACTTCATGTC
AvrII 79 CCTAGG C^CTAGG
3475 CCTAGG
BlnI 79 CCTAGG C^CTAGG
3475 CCTAGG
BlpI 769 GCTGAGC GC^T[ACGT]AGC
4161 GCTCAGC
Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC
4161 GCTCAGC
BsgI 1067 GTGCAG GTGCAG(16/14)
1911 GTGCAG
BsmI 213 GCATTC GAATGC(1/-1)
4294 GCATTC
BsrBI 1745 GAGCGG CCGCTC(-3/-3)
3454 GAGCGG
BstYI 1275 GGATCC [GA]^GATC[CT]
3922 GGATCT
Bsu36I 47 CCTGAGG CC^T[ACGT]AGG
3528 CCTCAGG
CelII 769 GCTGAGC GC^T[ACGT]AGC
4161 GCTCAGC
DraIII 1038 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2681 CACTTTGTG
DrdI 2956 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3800 GACAAGGCTGTC
Eam1105I 2282 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3978 GACTTCATGTC
Eco81I 47 CCTGAGG CC^T[ACGT]AGG
3528 CCTCAGG
EcoRV 1637 GATATC GAT^ATC
1849 GATATC
HaeII 228 AGCGCC [GA]GCGC^[CT]
1342 GGCGCT
MflI 1275 GGATCC [GA]^GATC[CT]
3922 GGATCT
MslI 2327 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2940 CATTAAAATG
SacI 1430 GAGCTC GAGCT^C
3329 GAGCTC
SspI 4481 AATATT AAT^ATT
4511 AATATT
StuI 2153 AGGCCT AGG^CCT
3478 AGGCCT
XhoII 1275 GGATCC [GA]^GATC[CT]
3922 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I