##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AscI Asp718I AvrII BcgI
BglI BglII BlnI BlpI Bpu1102I
BspDI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI DraIII EagI
EclXI Eco52I EcoRV FseI HpaI
KpnI MfeI MluI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PmeI PshAI PvuI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 4979 AACGTT AA^CGTT
AhdI 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4847 GTGCAC G^TGCAC
AocI 3721 CCTGAGG CC^T[ACGT]AGG
ApaI 336 GGGCCC GGGCC^C
ApaLI 4847 GTGCAC G^TGCAC
AseI 5871 ATTAAT AT^TAAT
AsnI 5871 ATTAAT AT^TAAT
Asp700I 2619 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1019 TGCGCA TGC^GCA
BbeI 1549 GGCGCC GGCGC^C
BbrPI 4446 CACGTG CAC^GTG
BciVI 3218 GGATAC GTATCC(6/5)
BsaI 1858 GAGACC GGTCTC(1/5)
BsiEI 4543 CGGTCG CG[GA][CT]^CG
BsiWI 3872 CGTACG C^GTACG
Bsp1407I 1799 TGTACA T^GTACA
BspHI 4349 TCATGA T^CATGA
BsrDI 5138 GCAATG GCAATG(2/0)
BsrGI 1799 TGTACA T^GTACA
BstEII 549 GGTGACC G^GT[ACGT]ACC
BstPI 549 GGTGACC G^GT[ACGT]ACC
Bsu36I 3721 CCTGAGG CC^T[ACGT]AGG
CpoI 3934 CGGACCG CG^G[AT]CCG
DraI 66 TTTAAA TTT^AAA
Eam1105I 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3721 CCTGAGG CC^T[ACGT]AGG
EcoO65I 549 GGTGACC G^GT[ACGT]ACC
EcoRI 5625 GAATTC G^AATTC
FspI 1019 TGCGCA TGC^GCA
KasI 1549 GGCGCC G^GCGCC
NarI 1549 GGCGCC GG^CGCC
PflFI 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4446 CACGTG CAC^GTG
PmlI 4446 CACGTG CAC^GTG
PshBI 5871 ATTAAT AT^TAAT
Psp1406I 4979 AACGTT AA^CGTT
RcaI 4349 TCATGA T^CATGA
RsrII 3934 CGGACCG CG^G[AT]CCG
SalI 3852 GTCGAC G^TCGAC
SfoI 1549 GGCGCC GGC^GCC
SmaI 4563 CCCGGG CCC^GGG
SspBI 1799 TGTACA T^GTACA
Tth111I 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4563 CCCGGG C^CCGGG
XmaI 4563 CCCGGG C^CCGGG
XmnI 2619 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 3354 GACGTC GACGT^C
3541 GACGTC
AgeI 1655 ACCGGT A^CCGGT
4541 ACCGGT
BclI 2315 TGATCA T^GATCA
4091 TGATCA
BsaBI 2088 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATCACCATC
BsmBI 3891 GAGACG CGTCTC(1/5)
4318 GAGACG
BsmI 2235 GAATGC GAATGC(1/-1)
5554 GCATTC
EcoT22I 2209 ATGCAT ATGCA^T
5066 ATGCAT
FbaI 2315 TGATCA T^GATCA
4091 TGATCA
HindIII 290 AAGCTT A^AGCTT
4642 AAGCTT
KspI 1217 CCGCGG CCGC^GG
3180 CCGCGG
MamI 2088 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATCACCATC
NheI 1435 GCTAGC G^CTAGC
3564 GCTAGC
NsiI 2209 ATGCAT ATGCA^T
5066 ATGCAT
PinAI 1655 ACCGGT A^CCGGT
4541 ACCGGT
SacII 1217 CCGCGG CCGC^GG
3180 CCGCGG
SapI 837 GAAGAGC GCTCTTC(1/4)
1147 GAAGAGC
StuI 977 AGGCCT AGG^CCT
1037 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflII AflIII AluI
AlwI AlwNI Aor51HI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII