################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## Acc65I AscI Asp718I AvrII BcgI BglI BglII BlnI BlpI Bpu1102I BspDI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI DraIII EagI EclXI Eco52I EcoRV FseI HpaI KpnI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PmeI PshAI PvuI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 4979 AACGTT AA^CGTT AhdI 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4847 GTGCAC G^TGCAC AocI 3721 CCTGAGG CC^T[ACGT]AGG ApaI 336 GGGCCC GGGCC^C ApaLI 4847 GTGCAC G^TGCAC AseI 5871 ATTAAT AT^TAAT AsnI 5871 ATTAAT AT^TAAT Asp700I 2619 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1019 TGCGCA TGC^GCA BbeI 1549 GGCGCC GGCGC^C BbrPI 4446 CACGTG CAC^GTG BciVI 3218 GGATAC GTATCC(6/5) BsaI 1858 GAGACC GGTCTC(1/5) BsiEI 4543 CGGTCG CG[GA][CT]^CG BsiWI 3872 CGTACG C^GTACG Bsp1407I 1799 TGTACA T^GTACA BspHI 4349 TCATGA T^CATGA BsrDI 5138 GCAATG GCAATG(2/0) BsrGI 1799 TGTACA T^GTACA BstEII 549 GGTGACC G^GT[ACGT]ACC BstPI 549 GGTGACC G^GT[ACGT]ACC Bsu36I 3721 CCTGAGG CC^T[ACGT]AGG CpoI 3934 CGGACCG CG^G[AT]CCG DraI 66 TTTAAA TTT^AAA Eam1105I 4186 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3721 CCTGAGG CC^T[ACGT]AGG EcoO65I 549 GGTGACC G^GT[ACGT]ACC EcoRI 5625 GAATTC G^AATTC FspI 1019 TGCGCA TGC^GCA KasI 1549 GGCGCC G^GCGCC NarI 1549 GGCGCC GG^CGCC PflFI 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4446 CACGTG CAC^GTG PmlI 4446 CACGTG CAC^GTG PshBI 5871 ATTAAT AT^TAAT Psp1406I 4979 AACGTT AA^CGTT RcaI 4349 TCATGA T^CATGA RsrII 3934 CGGACCG CG^G[AT]CCG SalI 3852 GTCGAC G^TCGAC SfoI 1549 GGCGCC GGC^GCC SmaI 4563 CCCGGG CCC^GGG SspBI 1799 TGTACA T^GTACA Tth111I 3936 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 4563 CCCGGG C^CCGGG XmaI 4563 CCCGGG C^CCGGG XmnI 2619 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 3354 GACGTC GACGT^C 3541 GACGTC AgeI 1655 ACCGGT A^CCGGT 4541 ACCGGT BclI 2315 TGATCA T^GATCA 4091 TGATCA BsaBI 2088 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3393 GATCACCATC BsmBI 3891 GAGACG CGTCTC(1/5) 4318 GAGACG BsmI 2235 GAATGC GAATGC(1/-1) 5554 GCATTC EcoT22I 2209 ATGCAT ATGCA^T 5066 ATGCAT FbaI 2315 TGATCA T^GATCA 4091 TGATCA HindIII 290 AAGCTT A^AGCTT 4642 AAGCTT KspI 1217 CCGCGG CCGC^GG 3180 CCGCGG MamI 2088 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3393 GATCACCATC NheI 1435 GCTAGC G^CTAGC 3564 GCTAGC NsiI 2209 ATGCAT ATGCA^T 5066 ATGCAT PinAI 1655 ACCGGT A^CCGGT 4541 ACCGGT SacII 1217 CCGCGG CCGC^GG 3180 CCGCGG SapI 837 GAAGAGC GCTCTTC(1/4) 1147 GAAGAGC StuI 977 AGGCCT AGG^CCT 1037 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI Aor51HI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspLU11I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII