################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI ApaI AscI Asp718I AviII BbeI BbrPI BfrI BglII BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 505 GGCGTC G[GA]^CG[CT]C Alw44I 3558 GTGCAC G^TGCAC ApaLI 3558 GTGCAC G^TGCAC AseI 4585 ATTAAT AT^TAAT AsnI 4585 ATTAAT AT^TAAT AspI 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 5971 CCTAGG C^CTAGG BamHI 884 GGATCC G^GATCC BbsI 5622 GAAGAC GAAGAC(2/6) BbvCI 4085 CCTCAGC CCTCAGC(-5/-2) BcgI 4671 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4439 TGATCA T^GATCA BglI 1904 GCCGTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 5971 CCTAGG C^CTAGG BpuAI 5622 GAAGAC GAAGAC(2/6) BsaHI 505 GGCGTC G[GA]^CG[CT]C BsaWI 6526 ACCGGA [AT]^CCGG[AT] BsgI 1067 GTGCAG GTGCAG(16/14) BsiEI 1902 CGGCCG CG[GA][CT]^CG BsrFI 2885 GCCGGT [GA]^CCGG[CT] BstAPI 998 GCACGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 2885 GCCGGT [GA]^CCGG[CT] EagI 1902 CGGCCG C^GGCCG EclXI 1902 CGGCCG C^GGCCG Eco52I 1902 CGGCCG C^GGCCG FbaI 4439 TGATCA T^GATCA Hin1I 505 GGCGTC G[GA]^CG[CT]C MfeI 6618 CAATTG C^AATTG MunI 6618 CAATTG C^AATTG NruI 3373 TCGCGA TCG^CGA PflFI 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 432 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 501 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4585 ATTAAT AT^TAAT SapI 861 GCTCTTC GCTCTTC(1/4) ScaI 1264 AGTACT AGT^ACT SexAI 3715 ACCTGGT A^CC[AT]GGT SmaI 1568 CCCGGG CCC^GGG SmlI 1084 CTTGAG C^T[CT][GA]AG SpeI 4598 ACTAGT A^CTAGT SphI 3566 GCATGC GCATG^C StuI 674 AGGCCT AGG^CCT Tth111I 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 432 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1568 CCCGGG C^CCGGG XmaI 1568 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AhdI 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4342 GACTCTGGGTC AocI 3973 CCTGAGG CC^T[ACGT]AGG 4324 CCTGAGG Aor51HI 118 AGCGCT AGC^GCT 5034 AGCGCT Asp700I 395 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3887 GAAATACTTC AspEI 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4342 GACTCTGGGTC AvaI 1568 CCCGGG C^[CT]CG[GA]G 4679 CTCGGG BciVI 126 GGATAC GTATCC(6/5) 1805 GTATCC BsaI 713 GAGACC GGTCTC(1/5) 4349 GGTCTC BseRI 1036 CTCCTC GAGGAG(10/8) 4729 GAGGAG BsmI 4267 GAATGC GAATGC(1/-1) 6289 GAATGC BsoBI 1568 CCCGGG C^[CT]CG[GA]G 4679 CTCGGG BspLU11I 967 ACATGT A^CATGT 6032 ACATGT BspMI 1784 ACCTGC ACCTGC(4/8) 4820 ACCTGC BsrDI 1957 CATTGC GCAATG(2/0) 727 GCAATG BssSI 5857 CACGAG CACGAG(-5/-1) 2051 CTCGTG BstEII 893 GGTCACC G^GT[ACGT]ACC 4864 GGTTACC BstPI 893 GGTCACC G^GT[ACGT]ACC 4864 GGTTACC Bsu36I 3973 CCTGAGG CC^T[ACGT]AGG 4324 CCTGAGG DraII 1563 AGGTCCC [GA]G^G[ACGT]CC[CT] 2943 AGGTCCT Eam1105I 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4342 GACTCTGGGTC Eco47III 118 AGCGCT AGC^GCT 5034 AGCGCT Eco81I 3973 CCTGAGG CC^T[ACGT]AGG 4324 CCTGAGG EcoNI 4964 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4978 CCTGAGCAAGG EcoO109I 1563 AGGTCCC [GA]G^G[ACGT]CC[CT] 2943 AGGTCCT EcoO65I 893 GGTCACC G^GT[ACGT]ACC 4864 GGTTACC EcoRI 5371 GAATTC G^AATTC 5558 GAATTC EcoRV 904 GATATC GAT^ATC 5474 GATATC FauI 1079 CCCGC CCCGC(4/6) 3413 GCGGG HgaI 3258 GACGC GACGC(5/10) 506 GCGTC HindIII 3491 AAGCTT A^AGCTT 3633 AAGCTT NdeI 3385 CATATG CA^TATG 6025 CATATG PpuMI 1563 AGGTCCC [GA]G^G[AT]CC[CT] 2943 AGGTCCT PstI 3746 CTGCAG CTGCA^G 4332 CTGCAG SacI 2089 GAGCTC GAGCT^C 4921 GAGCTC XbaI 363 TCTAGA T^CTAGA 6533 TCTAGA XcmI 1450 CCATGAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6156 CCATGGCCCACATGG XmnI 395 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3887 GAAATACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraIII DsaI EaeI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII