##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI ApaI AscI Asp718I AviII
BbeI BbrPI BfrI BglII BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 505 GGCGTC G[GA]^CG[CT]C
Alw44I 3558 GTGCAC G^TGCAC
ApaLI 3558 GTGCAC G^TGCAC
AseI 4585 ATTAAT AT^TAAT
AsnI 4585 ATTAAT AT^TAAT
AspI 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5971 CCTAGG C^CTAGG
BamHI 884 GGATCC G^GATCC
BbsI 5622 GAAGAC GAAGAC(2/6)
BbvCI 4085 CCTCAGC CCTCAGC(-5/-2)
BcgI 4671 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4439 TGATCA T^GATCA
BglI 1904 GCCGTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5971 CCTAGG C^CTAGG
BpuAI 5622 GAAGAC GAAGAC(2/6)
BsaHI 505 GGCGTC G[GA]^CG[CT]C
BsaWI 6526 ACCGGA [AT]^CCGG[AT]
BsgI 1067 GTGCAG GTGCAG(16/14)
BsiEI 1902 CGGCCG CG[GA][CT]^CG
BsrFI 2885 GCCGGT [GA]^CCGG[CT]
BstAPI 998 GCACGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 2885 GCCGGT [GA]^CCGG[CT]
EagI 1902 CGGCCG C^GGCCG
EclXI 1902 CGGCCG C^GGCCG
Eco52I 1902 CGGCCG C^GGCCG
FbaI 4439 TGATCA T^GATCA
Hin1I 505 GGCGTC G[GA]^CG[CT]C
MfeI 6618 CAATTG C^AATTG
MunI 6618 CAATTG C^AATTG
NruI 3373 TCGCGA TCG^CGA
PflFI 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 432 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 501 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4585 ATTAAT AT^TAAT
SapI 861 GCTCTTC GCTCTTC(1/4)
ScaI 1264 AGTACT AGT^ACT
SexAI 3715 ACCTGGT A^CC[AT]GGT
SmaI 1568 CCCGGG CCC^GGG
SmlI 1084 CTTGAG C^T[CT][GA]AG
SpeI 4598 ACTAGT A^CTAGT
SphI 3566 GCATGC GCATG^C
StuI 674 AGGCCT AGG^CCT
Tth111I 2411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 432 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1568 CCCGGG C^CCGGG
XmaI 1568 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AhdI 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4342 GACTCTGGGTC
AocI 3973 CCTGAGG CC^T[ACGT]AGG
4324 CCTGAGG
Aor51HI 118 AGCGCT AGC^GCT
5034 AGCGCT
Asp700I 395 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3887 GAAATACTTC
AspEI 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4342 GACTCTGGGTC
AvaI 1568 CCCGGG C^[CT]CG[GA]G
4679 CTCGGG
BciVI 126 GGATAC GTATCC(6/5)
1805 GTATCC
BsaI 713 GAGACC GGTCTC(1/5)
4349 GGTCTC
BseRI 1036 CTCCTC GAGGAG(10/8)
4729 GAGGAG
BsmI 4267 GAATGC GAATGC(1/-1)
6289 GAATGC
BsoBI 1568 CCCGGG C^[CT]CG[GA]G
4679 CTCGGG
BspLU11I 967 ACATGT A^CATGT
6032 ACATGT
BspMI 1784 ACCTGC ACCTGC(4/8)
4820 ACCTGC
BsrDI 1957 CATTGC GCAATG(2/0)
727 GCAATG
BssSI 5857 CACGAG CACGAG(-5/-1)
2051 CTCGTG
BstEII 893 GGTCACC G^GT[ACGT]ACC
4864 GGTTACC
BstPI 893 GGTCACC G^GT[ACGT]ACC
4864 GGTTACC
Bsu36I 3973 CCTGAGG CC^T[ACGT]AGG
4324 CCTGAGG
DraII 1563 AGGTCCC [GA]G^G[ACGT]CC[CT]
2943 AGGTCCT
Eam1105I 442 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4342 GACTCTGGGTC
Eco47III 118 AGCGCT AGC^GCT
5034 AGCGCT
Eco81I 3973 CCTGAGG CC^T[ACGT]AGG
4324 CCTGAGG
EcoNI 4964 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4978 CCTGAGCAAGG
EcoO109I 1563 AGGTCCC [GA]G^G[ACGT]CC[CT]
2943 AGGTCCT
EcoO65I 893 GGTCACC G^GT[ACGT]ACC
4864 GGTTACC
EcoRI 5371 GAATTC G^AATTC
5558 GAATTC
EcoRV 904 GATATC GAT^ATC
5474 GATATC
FauI 1079 CCCGC CCCGC(4/6)
3413 GCGGG
HgaI 3258 GACGC GACGC(5/10)
506 GCGTC
HindIII 3491 AAGCTT A^AGCTT
3633 AAGCTT
NdeI 3385 CATATG CA^TATG
6025 CATATG
PpuMI 1563 AGGTCCC [GA]G^G[AT]CC[CT]
2943 AGGTCCT
PstI 3746 CTGCAG CTGCA^G
4332 CTGCAG
SacI 2089 GAGCTC GAGCT^C
4921 GAGCTC
XbaI 363 TCTAGA T^CTAGA
6533 TCTAGA
XcmI 1450 CCATGAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6156 CCATGGCCCACATGG
XmnI 395 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3887 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraIII DsaI EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII