################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AflII AflIII Alw44I ApaLI AscI Asp718I AviII AvrII BalI BbrPI BfrI BlnI BsiEI BsiWI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI EclXI Eco52I EcoO65I FseI FspI HpaI KpnI MluI MluNI MscI NaeI NgoMIV NheI NotI NspV PacI PmaCI PmeI PmlI PvuI RcaI RsrII SalI SbfI SexAI SfuI SgrAI Sse8387I SspI StuI SwaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AclI 4565 AACGTT AA^CGTT AgeI 1803 ACCGGT A^CCGGT AhdI 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 2885 ATTAAT AT^TAAT AsnI 2885 ATTAAT AT^TAAT AspEI 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 178 GGATCC G^GATCC BcgI 2341 CGACAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 5280 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 5443 TACGTA [CT]AC^GT[GA] BsmBI 46 GAGACG CGTCTC(1/5) BsmI 5343 GAATGC GAATGC(1/-1) Bsp1407I 4994 TGTACA T^GTACA BspDI 3617 ATCGAT AT^CGAT BsrGI 4994 TGTACA T^GTACA BstAPI 1168 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 3617 ATCGAT AT^CGAT DraI 4454 TTTAAA TTT^AAA Eam1105I 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4928 GAATTC G^AATTC EcoRV 3020 GATATC GAT^ATC EcoT22I 5381 ATGCAT ATGCA^T HincII 3530 GTTGAC GT[CT]^[GA]AC HindII 3530 GTTGAC GT[CT]^[GA]AC HindIII 3795 AAGCTT A^AGCTT KspI 5292 CCGCGG CCGC^GG NcoI 2364 CCATGG C^CATGG NdeI 1010 CATATG CA^TATG NruI 202 TCGCGA TCG^CGA NsiI 5381 ATGCAT ATGCA^T PaeR7I 2622 CTCGAG C^TCGAG PflFI 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1803 ACCGGT A^CCGGT PshBI 2885 ATTAAT AT^TAAT Psp1406I 4565 AACGTT AA^CGTT SacII 5292 CCGCGG CCGC^GG ScaI 4328 AGTACT AGT^ACT SfiI 5279 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 5443 TACGTA TAC^GTA SpeI 5300 ACTAGT A^CTAGT SphI 4168 GCATGC GCATG^C SspBI 4994 TGTACA T^GTACA Tth111I 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2622 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 171 TCCGGA T^CCGGA 2919 TCCGGA Aor51HI 717 AGCGCT AGC^GCT 3465 AGCGCT Asp700I 1490 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2969 GAAGGGATTC BbeI 1547 GGCGCC GGCGC^C 2593 GGCGCC BlpI 3754 GCTGAGC GC^T[ACGT]AGC 5106 GCTCAGC Bpu1102I 3754 GCTGAGC GC^T[ACGT]AGC 5106 GCTCAGC BsaI 4313 GGTCTC GGTCTC(1/5) 5351 GGTCTC BseAI 171 TCCGGA T^CCGGA 2919 TCCGGA BspEI 171 TCCGGA T^CCGGA 2919 TCCGGA BspMI 2490 ACCTGC ACCTGC(4/8) 3775 GCAGGT BsrBI 93 GAGCGG CCGCTC(-3/-3) 4247 GAGCGG BsrDI 1710 CATTGC GCAATG(2/0) 3349 CATTGC BsrFI 1803 ACCGGT [GA]^CCGG[CT] 4906 GCCGGT BssSI 68 CACGAG CACGAG(-5/-1) 1085 CTCGTG CelII 3754 GCTGAGC GC^T[ACGT]AGC 5106 GCTCAGC Cfr10I 1803 ACCGGT [GA]^CCGG[CT] 4906 GCCGGT DraIII 1338 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4671 CACATAGTG DrdI 393 GACTTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 773 GACAATCCGGTC EaeI 64 CGGCCA [CT]^GGCC[GA] 278 TGGCCG Eco47III 717 AGCGCT AGC^GCT 3465 AGCGCT KasI 1547 GGCGCC G^GCGCC 2593 GGCGCC MaeII 4566 ACGT A^CGT 5444 ACGT MfeI 2352 CAATTG C^AATTG 5590 CAATTG MroI 171 TCCGGA T^CCGGA 2919 TCCGGA MunI 2352 CAATTG C^AATTG 5590 CAATTG NarI 1547 GGCGCC GG^CGCC 2593 GGCGCC SapI 5650 GAAGAGC GCTCTTC(1/4) 5081 GCTCTTC SfoI 1547 GGCGCC GGC^GCC 2593 GGCGCC XbaI 364 TCTAGA T^CTAGA 1852 TCTAGA XcmI 3990 CCAAGTTAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5092 CCAAAGGCTGGCTGG XmnI 1490 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2969 GAAGGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI