##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII AflIII Alw44I
ApaLI AscI Asp718I AviII AvrII
BalI BbrPI BfrI BlnI BsiEI
BsiWI BspHI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
EagI EclXI Eco52I EcoO65I FseI
FspI HpaI KpnI MluI MluNI
MscI NaeI NgoMIV NheI NotI
NspV PacI PmaCI PmeI PmlI
PvuI RcaI RsrII SalI SbfI
SexAI SfuI SgrAI Sse8387I SspI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 4565 AACGTT AA^CGTT
AgeI 1803 ACCGGT A^CCGGT
AhdI 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2885 ATTAAT AT^TAAT
AsnI 2885 ATTAAT AT^TAAT
AspEI 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 178 GGATCC G^GATCC
BcgI 2341 CGACAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 5280 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 5443 TACGTA [CT]AC^GT[GA]
BsmBI 46 GAGACG CGTCTC(1/5)
BsmI 5343 GAATGC GAATGC(1/-1)
Bsp1407I 4994 TGTACA T^GTACA
BspDI 3617 ATCGAT AT^CGAT
BsrGI 4994 TGTACA T^GTACA
BstAPI 1168 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3617 ATCGAT AT^CGAT
DraI 4454 TTTAAA TTT^AAA
Eam1105I 4340 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4928 GAATTC G^AATTC
EcoRV 3020 GATATC GAT^ATC
EcoT22I 5381 ATGCAT ATGCA^T
HincII 3530 GTTGAC GT[CT]^[GA]AC
HindII 3530 GTTGAC GT[CT]^[GA]AC
HindIII 3795 AAGCTT A^AGCTT
KspI 5292 CCGCGG CCGC^GG
NcoI 2364 CCATGG C^CATGG
NdeI 1010 CATATG CA^TATG
NruI 202 TCGCGA TCG^CGA
NsiI 5381 ATGCAT ATGCA^T
PaeR7I 2622 CTCGAG C^TCGAG
PflFI 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1803 ACCGGT A^CCGGT
PshBI 2885 ATTAAT AT^TAAT
Psp1406I 4565 AACGTT AA^CGTT
SacII 5292 CCGCGG CCGC^GG
ScaI 4328 AGTACT AGT^ACT
SfiI 5279 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 5443 TACGTA TAC^GTA
SpeI 5300 ACTAGT A^CTAGT
SphI 4168 GCATGC GCATG^C
SspBI 4994 TGTACA T^GTACA
Tth111I 667 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2622 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 171 TCCGGA T^CCGGA
2919 TCCGGA
Aor51HI 717 AGCGCT AGC^GCT
3465 AGCGCT
Asp700I 1490 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2969 GAAGGGATTC
BbeI 1547 GGCGCC GGCGC^C
2593 GGCGCC
BlpI 3754 GCTGAGC GC^T[ACGT]AGC
5106 GCTCAGC
Bpu1102I 3754 GCTGAGC GC^T[ACGT]AGC
5106 GCTCAGC
BsaI 4313 GGTCTC GGTCTC(1/5)
5351 GGTCTC
BseAI 171 TCCGGA T^CCGGA
2919 TCCGGA
BspEI 171 TCCGGA T^CCGGA
2919 TCCGGA
BspMI 2490 ACCTGC ACCTGC(4/8)
3775 GCAGGT
BsrBI 93 GAGCGG CCGCTC(-3/-3)
4247 GAGCGG
BsrDI 1710 CATTGC GCAATG(2/0)
3349 CATTGC
BsrFI 1803 ACCGGT [GA]^CCGG[CT]
4906 GCCGGT
BssSI 68 CACGAG CACGAG(-5/-1)
1085 CTCGTG
CelII 3754 GCTGAGC GC^T[ACGT]AGC
5106 GCTCAGC
Cfr10I 1803 ACCGGT [GA]^CCGG[CT]
4906 GCCGGT
DraIII 1338 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4671 CACATAGTG
DrdI 393 GACTTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
773 GACAATCCGGTC
EaeI 64 CGGCCA [CT]^GGCC[GA]
278 TGGCCG
Eco47III 717 AGCGCT AGC^GCT
3465 AGCGCT
KasI 1547 GGCGCC G^GCGCC
2593 GGCGCC
MaeII 4566 ACGT A^CGT
5444 ACGT
MfeI 2352 CAATTG C^AATTG
5590 CAATTG
MroI 171 TCCGGA T^CCGGA
2919 TCCGGA
MunI 2352 CAATTG C^AATTG
5590 CAATTG
NarI 1547 GGCGCC GG^CGCC
2593 GGCGCC
SapI 5650 GAAGAGC GCTCTTC(1/4)
5081 GCTCTTC
SfoI 1547 GGCGCC GGC^GCC
2593 GGCGCC
XbaI 364 TCTAGA T^CTAGA
1852 TCTAGA
XcmI 3990 CCAAGTTAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5092 CCAAAGGCTGGCTGG
XmnI 1490 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2969 GAAGGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI