################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AccIII AclI AgeI AseI AsnI AviII BciVI BsaBI BseAI BsmI BspDI BspEI BspLU11I BssSI Bst1107I BstZ17I ClaI CpoI EcoRI EcoT22I FspI HincII HindII HindIII HpaI MamI MluI MroI NdeI NheI NruI NsiI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SgrAI SnaBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 1631 GACGTC GACGT^C Acc65I 2743 GGTACC G^GTACC AccI 3375 GTCTAC GT^[AC][GT]AC AflII 3897 CTTAAG C^TTAAG AflIII 2212 ACACGT A^C[GA][CT]GT AocI 3786 CCTCAGG CC^T[ACGT]AGG Aor51HI 3258 AGCGCT AGC^GCT Asp700I 1364 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2743 GGTACC G^GTACC BamHI 3323 GGATCC G^GATCC BbrPI 2213 CACGTG CAC^GTG BbvCI 970 GCTGAGG CCTCAGC(-5/-2) BclI 1978 TGATCA T^GATCA BfrI 3897 CTTAAG C^TTAAG BglII 3053 AGATCT A^GATCT BlpI 803 GCTGAGC GC^T[ACGT]AGC Bpu1102I 803 GCTGAGC GC^T[ACGT]AGC Bsp1407I 394 TGTACA T^GTACA BspHI 3274 TCATGA T^CATGA BsrGI 394 TGTACA T^GTACA BstBI 176 TTCGAA TT^CGAA BstEII 3185 GGTCACC G^GT[ACGT]ACC BstPI 3185 GGTCACC G^GT[ACGT]ACC Bsu36I 3786 CCTCAGG CC^T[ACGT]AGG CelII 803 GCTGAGC GC^T[ACGT]AGC DraI 1406 TTTAAA TTT^AAA EagI 785 CGGCCG C^GGCCG EclXI 785 CGGCCG C^GGCCG Eco47III 3258 AGCGCT AGC^GCT Eco52I 785 CGGCCG C^GGCCG Eco81I 3786 CCTCAGG CC^T[ACGT]AGG EcoO65I 3185 GGTCACC G^GT[ACGT]ACC FbaI 1978 TGATCA T^GATCA FseI 667 GGCCGGCC GGCCGG^CC KpnI 2743 GGTACC GGTAC^C MfeI 3689 CAATTG C^AATTG MunI 3689 CAATTG C^AATTG NaeI 668 GCCGGC GCC^GGC NcoI 2932 CCATGG C^CATGG NgoMIV 668 GCCGGC G^CCGGC NotI 784 GCGGCCGC GC^GGCCGC NspV 176 TTCGAA TT^CGAA PaeR7I 2682 CTCGAG C^TCGAG PmaCI 2213 CACGTG CAC^GTG PmlI 2213 CACGTG CAC^GTG RcaI 3274 TCATGA T^CATGA SacI 3008 GAGCTC GAGCT^C SapI 3005 GAAGAGC GCTCTTC(1/4) SbfI 3716 CCTGCAGG CCTGCA^GG ScaI 4282 AGTACT AGT^ACT SexAI 4648 ACCTGGT A^CC[AT]GGT SfuI 176 TTCGAA TT^CGAA SpeI 1485 ACTAGT A^CTAGT Sse8387I 3716 CCTGCAGG CCTGCA^GG SspBI 394 TGTACA T^GTACA TfiI 3105 GATTC G^A[AT]TC XhoI 2682 CTCGAG C^TCGAG XmnI 1364 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 179 GAATTT [GA]^AATT[CT] 1131 GAATTT AhdI 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3648 GACGAGCTGTC Alw44I 1788 GTGCAC G^TGCAC 3798 GTGCAC ApaLI 1788 GTGCAC G^TGCAC 3798 GTGCAC ApoI 179 GAATTT [GA]^AATT[CT] 1131 GAATTT AscI 853 GGCGCGCC GG^CGCGCC 897 GGCGCGCC AspEI 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3648 GACGAGCTGTC AvrII 3287 CCTAGG C^CTAGG 3995 CCTAGG BcgI 553 CGAGAAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 994 CGAGCAGCATGC BlnI 3287 CCTAGG C^CTAGG 3995 CCTAGG BsaI 3072 GAGACC GGTCTC(1/5) 3479 GGTCTC BsaWI 2459 ACCGGA [AT]^CCGG[AT] 4150 ACCGGA BsiWI 2164 CGTACG C^GTACG 3970 CGTACG BsmBI 4061 CGTCTC CGTCTC(1/5) 3987 GAGACG BsrDI 1141 GCAATG GCAATG(2/0) 2920 GCAATG Eam1105I 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3648 GACGAGCTGTC EcoNI 2624 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3287 CCTAGGAAAGG EcoRV 2667 GATATC GAT^ATC 3534 GATATC SphI 1000 GCATGC GCATG^C 3358 GCATGC StuI 2988 AGGCCT AGG^CCT 4310 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI