##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AccIII      AclI        AgeI        AseI        AsnI        
AviII       BciVI       BsaBI       BseAI       BsmI        
BspDI       BspEI       BspLU11I    BssSI       Bst1107I    
BstZ17I     ClaI        CpoI        EcoRI       EcoT22I     
FspI        HincII      HindII      HindIII     HpaI        
MamI        MluI        MroI        NdeI        NheI        
NruI        NsiI        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SgrAI       SnaBI       SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        1631  GACGTC               GACGT^C
Acc65I       2743  GGTACC               G^GTACC
AccI         3375  GTCTAC               GT^[AC][GT]AC
AflII        3897  CTTAAG               C^TTAAG
AflIII       2212  ACACGT               A^C[GA][CT]GT
AocI         3786  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      3258  AGCGCT               AGC^GCT
Asp700I      1364  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2743  GGTACC               G^GTACC
BamHI        3323  GGATCC               G^GATCC
BbrPI        2213  CACGTG               CAC^GTG
BbvCI         970  GCTGAGG              CCTCAGC(-5/-2)
BclI         1978  TGATCA               T^GATCA
BfrI         3897  CTTAAG               C^TTAAG
BglII        3053  AGATCT               A^GATCT
BlpI          803  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      803  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I      394  TGTACA               T^GTACA
BspHI        3274  TCATGA               T^CATGA
BsrGI         394  TGTACA               T^GTACA
BstBI         176  TTCGAA               TT^CGAA
BstEII       3185  GGTCACC              G^GT[ACGT]ACC
BstPI        3185  GGTCACC              G^GT[ACGT]ACC
Bsu36I       3786  CCTCAGG              CC^T[ACGT]AGG
CelII         803  GCTGAGC              GC^T[ACGT]AGC
DraI         1406  TTTAAA               TTT^AAA
EagI          785  CGGCCG               C^GGCCG
EclXI         785  CGGCCG               C^GGCCG
Eco47III     3258  AGCGCT               AGC^GCT
Eco52I        785  CGGCCG               C^GGCCG
Eco81I       3786  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      3185  GGTCACC              G^GT[ACGT]ACC
FbaI         1978  TGATCA               T^GATCA
FseI          667  GGCCGGCC             GGCCGG^CC
KpnI         2743  GGTACC               GGTAC^C
MfeI         3689  CAATTG               C^AATTG
MunI         3689  CAATTG               C^AATTG
NaeI          668  GCCGGC               GCC^GGC
NcoI         2932  CCATGG               C^CATGG
NgoMIV        668  GCCGGC               G^CCGGC
NotI          784  GCGGCCGC             GC^GGCCGC
NspV          176  TTCGAA               TT^CGAA
PaeR7I       2682  CTCGAG               C^TCGAG
PmaCI        2213  CACGTG               CAC^GTG
PmlI         2213  CACGTG               CAC^GTG
RcaI         3274  TCATGA               T^CATGA
SacI         3008  GAGCTC               GAGCT^C
SapI         3005  GAAGAGC              GCTCTTC(1/4)
SbfI         3716  CCTGCAGG             CCTGCA^GG
ScaI         4282  AGTACT               AGT^ACT
SexAI        4648  ACCTGGT              A^CC[AT]GGT
SfuI          176  TTCGAA               TT^CGAA
SpeI         1485  ACTAGT               A^CTAGT
Sse8387I     3716  CCTGCAGG             CCTGCA^GG
SspBI         394  TGTACA               T^GTACA
TfiI         3105  GATTC                G^A[AT]TC
XhoI         2682  CTCGAG               C^TCGAG
XmnI         1364  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          179  GAATTT               [GA]^AATT[CT]
             1131  GAATTT              
AhdI          459  GACAGCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3648  GACGAGCTGTC         
Alw44I       1788  GTGCAC               G^TGCAC
             3798  GTGCAC              
ApaLI        1788  GTGCAC               G^TGCAC
             3798  GTGCAC              
ApoI          179  GAATTT               [GA]^AATT[CT]
             1131  GAATTT              
AscI          853  GGCGCGCC             GG^CGCGCC
              897  GGCGCGCC            
AspEI         459  GACAGCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3648  GACGAGCTGTC         
AvrII        3287  CCTAGG               C^CTAGG
             3995  CCTAGG              
BcgI          553  CGAGAAAGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              994  CGAGCAGCATGC        
BlnI         3287  CCTAGG               C^CTAGG
             3995  CCTAGG              
BsaI         3072  GAGACC               GGTCTC(1/5)
             3479  GGTCTC              
BsaWI        2459  ACCGGA               [AT]^CCGG[AT]
             4150  ACCGGA              
BsiWI        2164  CGTACG               C^GTACG
             3970  CGTACG              
BsmBI        4061  CGTCTC               CGTCTC(1/5)
             3987  GAGACG              
BsrDI        1141  GCAATG               GCAATG(2/0)
             2920  GCAATG              
Eam1105I      459  GACAGCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3648  GACGAGCTGTC         
EcoNI        2624  CCTGCGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3287  CCTAGGAAAGG         
EcoRV        2667  GATATC               GAT^ATC
             3534  GATATC              
SphI         1000  GCATGC               GCATG^C
             3358  GCATGC              
StuI         2988  AGGCCT               AGG^CCT
             4310  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssHII      BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SfoI        SmaI        SmlI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI