##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AclI AgeI AseI AsnI
AviII BciVI BsaBI BseAI BsmI
BspDI BspEI BspLU11I BssSI Bst1107I
BstZ17I ClaI CpoI EcoRI EcoT22I
FspI HincII HindII HindIII HpaI
MamI MluI MroI NdeI NheI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SgrAI SnaBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1631 GACGTC GACGT^C
Acc65I 2743 GGTACC G^GTACC
AccI 3375 GTCTAC GT^[AC][GT]AC
AflII 3897 CTTAAG C^TTAAG
AflIII 2212 ACACGT A^C[GA][CT]GT
AocI 3786 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3258 AGCGCT AGC^GCT
Asp700I 1364 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2743 GGTACC G^GTACC
BamHI 3323 GGATCC G^GATCC
BbrPI 2213 CACGTG CAC^GTG
BbvCI 970 GCTGAGG CCTCAGC(-5/-2)
BclI 1978 TGATCA T^GATCA
BfrI 3897 CTTAAG C^TTAAG
BglII 3053 AGATCT A^GATCT
BlpI 803 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 803 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 394 TGTACA T^GTACA
BspHI 3274 TCATGA T^CATGA
BsrGI 394 TGTACA T^GTACA
BstBI 176 TTCGAA TT^CGAA
BstEII 3185 GGTCACC G^GT[ACGT]ACC
BstPI 3185 GGTCACC G^GT[ACGT]ACC
Bsu36I 3786 CCTCAGG CC^T[ACGT]AGG
CelII 803 GCTGAGC GC^T[ACGT]AGC
DraI 1406 TTTAAA TTT^AAA
EagI 785 CGGCCG C^GGCCG
EclXI 785 CGGCCG C^GGCCG
Eco47III 3258 AGCGCT AGC^GCT
Eco52I 785 CGGCCG C^GGCCG
Eco81I 3786 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3185 GGTCACC G^GT[ACGT]ACC
FbaI 1978 TGATCA T^GATCA
FseI 667 GGCCGGCC GGCCGG^CC
KpnI 2743 GGTACC GGTAC^C
MfeI 3689 CAATTG C^AATTG
MunI 3689 CAATTG C^AATTG
NaeI 668 GCCGGC GCC^GGC
NcoI 2932 CCATGG C^CATGG
NgoMIV 668 GCCGGC G^CCGGC
NotI 784 GCGGCCGC GC^GGCCGC
NspV 176 TTCGAA TT^CGAA
PaeR7I 2682 CTCGAG C^TCGAG
PmaCI 2213 CACGTG CAC^GTG
PmlI 2213 CACGTG CAC^GTG
RcaI 3274 TCATGA T^CATGA
SacI 3008 GAGCTC GAGCT^C
SapI 3005 GAAGAGC GCTCTTC(1/4)
SbfI 3716 CCTGCAGG CCTGCA^GG
ScaI 4282 AGTACT AGT^ACT
SexAI 4648 ACCTGGT A^CC[AT]GGT
SfuI 176 TTCGAA TT^CGAA
SpeI 1485 ACTAGT A^CTAGT
Sse8387I 3716 CCTGCAGG CCTGCA^GG
SspBI 394 TGTACA T^GTACA
TfiI 3105 GATTC G^A[AT]TC
XhoI 2682 CTCGAG C^TCGAG
XmnI 1364 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 179 GAATTT [GA]^AATT[CT]
1131 GAATTT
AhdI 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3648 GACGAGCTGTC
Alw44I 1788 GTGCAC G^TGCAC
3798 GTGCAC
ApaLI 1788 GTGCAC G^TGCAC
3798 GTGCAC
ApoI 179 GAATTT [GA]^AATT[CT]
1131 GAATTT
AscI 853 GGCGCGCC GG^CGCGCC
897 GGCGCGCC
AspEI 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3648 GACGAGCTGTC
AvrII 3287 CCTAGG C^CTAGG
3995 CCTAGG
BcgI 553 CGAGAAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
994 CGAGCAGCATGC
BlnI 3287 CCTAGG C^CTAGG
3995 CCTAGG
BsaI 3072 GAGACC GGTCTC(1/5)
3479 GGTCTC
BsaWI 2459 ACCGGA [AT]^CCGG[AT]
4150 ACCGGA
BsiWI 2164 CGTACG C^GTACG
3970 CGTACG
BsmBI 4061 CGTCTC CGTCTC(1/5)
3987 GAGACG
BsrDI 1141 GCAATG GCAATG(2/0)
2920 GCAATG
Eam1105I 459 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3648 GACGAGCTGTC
EcoNI 2624 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3287 CCTAGGAAAGG
EcoRV 2667 GATATC GAT^ATC
3534 GATATC
SphI 1000 GCATGC GCATG^C
3358 GCATGC
StuI 2988 AGGCCT AGG^CCT
4310 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI