##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflIII AgeI Aor51HI
AscI Asp718I AviII AvrII BbeI
BbrPI BbsI BglII BlnI BpuAI
BsaAI BsiWI BsmI BspHI BspLU11I
BsrDI BssHII BstBI CpoI Eco47III
EcoRI EcoT22I FseI FspI HpaI
KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 3031 GACGTC GACGT^C
AflII 541 CTTAAG C^TTAAG
AspI 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3689 GCATGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 541 CTTAAG C^TTAAG
BglI 1182 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 266 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2004 CGGCCG CG[GA][CT]^CG
Bsp1407I 1299 TGTACA T^GTACA
BspDI 3717 ATCGAT AT^CGAT
BsrGI 1299 TGTACA T^GTACA
Bst1107I 2475 GTATAC GTA^TAC
BstEII 2712 GGTGACC G^GT[ACGT]ACC
BstPI 2712 GGTGACC G^GT[ACGT]ACC
BstZ17I 2475 GTATAC GTA^TAC
ClaI 3717 ATCGAT AT^CGAT
EagI 2004 CGGCCG C^GGCCG
EclXI 2004 CGGCCG C^GGCCG
Eco52I 2004 CGGCCG C^GGCCG
EcoO65I 2712 GGTGACC G^GT[ACGT]ACC
KspI 1483 CCGCGG CCGC^GG
MamI 266 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2174 CAATTG C^AATTG
MunI 2174 CAATTG C^AATTG
PflFI 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1483 CCGCGG CCGC^GG
SbfI 3200 CCTGCAGG CCTGCA^GG
SexAI 3922 ACCAGGT A^CC[AT]GGT
Sse8387I 3200 CCTGCAGG CCTGCA^GG
SspBI 1299 TGTACA T^GTACA
SspI 4364 AATATT AAT^ATT
Tth111I 1925 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 1484 CGCG CG^CG
3631 CGCG
AccIII 2281 TCCGGA T^CCGGA
3008 TCCGGA
AcyI 1493 GACGCC G[GA]^CG[CT]C
3031 GACGTC
Alw44I 635 GTGCAC G^TGCAC
3626 GTGCAC
AocI 62 CCTCAGG CC^T[ACGT]AGG
932 CCTCAGG
ApaLI 635 GTGCAC G^TGCAC
3626 GTGCAC
AseI 4361 ATTAAT AT^TAAT
5155 ATTAAT
AsnI 4361 ATTAAT AT^TAAT
5155 ATTAAT
Asp700I 4023 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4883 GAACTGTTTC
BalI 1798 TGGCCA TGG^CCA
3568 TGGCCA
BciVI 422 GTATCC GTATCC(6/5)
3888 GTATCC
BclI 2963 TGATCA T^GATCA
4805 TGATCA
BsaHI 1493 GACGCC G[GA]^CG[CT]C
3031 GACGTC
BseAI 2281 TCCGGA T^CCGGA
3008 TCCGGA
BspEI 2281 TCCGGA T^CCGGA
3008 TCCGGA
BsrBI 1900 CCGCTC CCGCTC(-3/-3)
2838 CCGCTC
BsrFI 1091 ACCGGC [GA]^CCGG[CT]
1975 ACCGGC
BstUI 1484 CGCG CG^CG
3631 CGCG
Bsu36I 62 CCTCAGG CC^T[ACGT]AGG
932 CCTCAGG
Cfr10I 1091 ACCGGC [GA]^CCGG[CT]
1975 ACCGGC
DraI 1672 TTTAAA TTT^AAA
1741 TTTAAA
DraIII 2329 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3655 CACCTAGTG
Eco81I 62 CCTCAGG CC^T[ACGT]AGG
932 CCTCAGG
EcoRV 1509 GATATC GAT^ATC
5039 GATATC
FbaI 2963 TGATCA T^GATCA
4805 TGATCA
Hin1I 1493 GACGCC G[GA]^CG[CT]C
3031 GACGTC
HindIII 1387 AAGCTT A^AGCTT
3369 AAGCTT
MluNI 1798 TGGCCA TGG^CCA
3568 TGGCCA
MroI 2281 TCCGGA T^CCGGA
3008 TCCGGA
MscI 1798 TGGCCA TGG^CCA
3568 TGGCCA
MvnI 1484 CGCG CG^CG
3631 CGCG
PshBI 4361 ATTAAT AT^TAAT
5155 ATTAAT
SacI 3711 GAGCTC GAGCT^C
4265 GAGCTC
XbaI 2798 TCTAGA T^CTAGA
4031 TCTAGA
XcmI 4275 CCAGAACGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4303 CCAAGGTCTACCTGG
XmnI 4023 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4883 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApaI ApoI
AspEI AspHI AvaI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoI XhoII