##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AvaI AviII BamHI BanI
BbeI BbrPI BcgI BcnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BstBI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII HapII Hin1I HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 2892 CGCG CG^CG
AflII 2953 CTTAAG C^TTAAG
ApaI 3811 GGGCCC GGGCC^C
AspI 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1264 TGGCCA TGG^CCA
BbvCI 1867 GCTGAGG CCTCAGC(-5/-2)
BciVI 767 GTATCC GTATCC(6/5)
BclI 1316 TGATCA T^GATCA
BfrI 2953 CTTAAG C^TTAAG
BglI 432 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 414 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 414 GCTGAGC GC^T[ACGT]AGC
BsaAI 1059 TACGTG [CT]AC^GT[GA]
BsaBI 3748 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 99 GGTCTC GGTCTC(1/5)
Bst1107I 3394 GTATAC GTA^TAC
BstAPI 419 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1184 GGTAACC G^GT[ACGT]ACC
BstPI 1184 GGTAACC G^GT[ACGT]ACC
BstUI 2892 CGCG CG^CG
BstZ17I 3394 GTATAC GTA^TAC
CelII 414 GCTGAGC GC^T[ACGT]AGC
DraIII 538 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1184 GGTAACC G^GT[ACGT]ACC
EcoRI 2099 GAATTC G^AATTC
FbaI 1316 TGATCA T^GATCA
HgaI 394 GACGC GACGC(5/10)
HincII 1146 GTCGAC GT[CT]^[GA]AC
HindII 1146 GTCGAC GT[CT]^[GA]AC
MamI 3748 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1264 TGGCCA TGG^CCA
MscI 1264 TGGCCA TGG^CCA
MslI 2271 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2892 CGCG CG^CG
NcoI 1042 CCATGG C^CATGG
NheI 1888 GCTAGC G^CTAGC
PflFI 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2401 GGGACCC [GA]G^G[AT]CC[CT]
SalI 1146 GTCGAC G^TCGAC
ScaI 1349 AGTACT AGT^ACT
SspI 4384 AATATT AAT^ATT
Tth111I 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 775 ACATGT A^C[GA][CT]GT
1664 ACATGT
AhdI 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4283 GACTGGTTGTC
AocI 1464 CCTGAGG CC^T[ACGT]AGG
3694 CCTGAGG
AseI 1925 ATTAAT AT^TAAT
2086 ATTAAT
AsnI 1925 ATTAAT AT^TAAT
2086 ATTAAT
Asp700I 930 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2610 GAAGTTTTTC
AspEI 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4283 GACTGGTTGTC
AvrII 2444 CCTAGG C^CTAGG
3100 CCTAGG
BlnI 2444 CCTAGG C^CTAGG
3100 CCTAGG
BsgI 6 CTGCAC GTGCAG(16/14)
2179 CTGCAC
BspLU11I 775 ACATGT A^CATGT
1664 ACATGT
BssSI 1836 CTCGTG CACGAG(-5/-1)
1899 CTCGTG
Bsu36I 1464 CCTGAGG CC^T[ACGT]AGG
3694 CCTGAGG
CfoI 1027 GCGC GCG^C
2893 GCGC
DraI 780 TTTAAA TTT^AAA
4591 TTTAAA
DrdI 357 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4011 GACTTCTGTGTC
DsaI 1042 CCATGG C^C[GA][CT]GG
4484 CCACGG
EaeI 1264 TGGCCA [CT]^GGCC[GA]
4487 CGGCCA
Eam1105I 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4283 GACTGGTTGTC
Eco81I 1464 CCTGAGG CC^T[ACGT]AGG
3694 CCTGAGG
EcoNI 90 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2052 CCTTTCCCAGG
EcoRV 2595 GATATC GAT^ATC
3021 GATATC
EcoT22I 3543 ATGCAT ATGCA^T
4607 ATGCAT
FauI 867 CCCGC CCCGC(4/6)
1746 CCCGC
HhaI 1027 GCGC GCG^C
2893 GCGC
HinP1I 1027 GCGC G^CGC
2893 GCGC
NsiI 3543 ATGCAT ATGCA^T
4607 ATGCAT
PshBI 1925 ATTAAT AT^TAAT
2086 ATTAAT
PstI 2642 CTGCAG CTGCA^G
2848 CTGCAG
SmlI 2953 CTTAAG C^T[CT][GA]AG
3109 CTTGAG
StuI 2065 AGGCCT AGG^CCT
2093 AGGCCT
XbaI 1790 TCTAGA T^CTAGA
3258 TCTAGA
XcmI 1189 CCAGAGAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1281 CCAGAACTGACCTGG
XmnI 930 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2610 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII