################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AvaI AviII BamHI BanI BbeI BbrPI BcgI BcnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstBI Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I FseI FspI HaeII HapII Hin1I HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 2892 CGCG CG^CG AflII 2953 CTTAAG C^TTAAG ApaI 3811 GGGCCC GGGCC^C AspI 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1264 TGGCCA TGG^CCA BbvCI 1867 GCTGAGG CCTCAGC(-5/-2) BciVI 767 GTATCC GTATCC(6/5) BclI 1316 TGATCA T^GATCA BfrI 2953 CTTAAG C^TTAAG BglI 432 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 414 GCTGAGC GC^T[ACGT]AGC Bpu1102I 414 GCTGAGC GC^T[ACGT]AGC BsaAI 1059 TACGTG [CT]AC^GT[GA] BsaBI 3748 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 99 GGTCTC GGTCTC(1/5) Bst1107I 3394 GTATAC GTA^TAC BstAPI 419 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1184 GGTAACC G^GT[ACGT]ACC BstPI 1184 GGTAACC G^GT[ACGT]ACC BstUI 2892 CGCG CG^CG BstZ17I 3394 GTATAC GTA^TAC CelII 414 GCTGAGC GC^T[ACGT]AGC DraIII 538 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1184 GGTAACC G^GT[ACGT]ACC EcoRI 2099 GAATTC G^AATTC FbaI 1316 TGATCA T^GATCA HgaI 394 GACGC GACGC(5/10) HincII 1146 GTCGAC GT[CT]^[GA]AC HindII 1146 GTCGAC GT[CT]^[GA]AC MamI 3748 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1264 TGGCCA TGG^CCA MscI 1264 TGGCCA TGG^CCA MslI 2271 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 2892 CGCG CG^CG NcoI 1042 CCATGG C^CATGG NheI 1888 GCTAGC G^CTAGC PflFI 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2401 GGGACCC [GA]G^G[AT]CC[CT] SalI 1146 GTCGAC G^TCGAC ScaI 1349 AGTACT AGT^ACT SspI 4384 AATATT AAT^ATT Tth111I 885 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflIII 775 ACATGT A^C[GA][CT]GT 1664 ACATGT AhdI 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4283 GACTGGTTGTC AocI 1464 CCTGAGG CC^T[ACGT]AGG 3694 CCTGAGG AseI 1925 ATTAAT AT^TAAT 2086 ATTAAT AsnI 1925 ATTAAT AT^TAAT 2086 ATTAAT Asp700I 930 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2610 GAAGTTTTTC AspEI 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4283 GACTGGTTGTC AvrII 2444 CCTAGG C^CTAGG 3100 CCTAGG BlnI 2444 CCTAGG C^CTAGG 3100 CCTAGG BsgI 6 CTGCAC GTGCAG(16/14) 2179 CTGCAC BspLU11I 775 ACATGT A^CATGT 1664 ACATGT BssSI 1836 CTCGTG CACGAG(-5/-1) 1899 CTCGTG Bsu36I 1464 CCTGAGG CC^T[ACGT]AGG 3694 CCTGAGG CfoI 1027 GCGC GCG^C 2893 GCGC DraI 780 TTTAAA TTT^AAA 4591 TTTAAA DrdI 357 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4011 GACTTCTGTGTC DsaI 1042 CCATGG C^C[GA][CT]GG 4484 CCACGG EaeI 1264 TGGCCA [CT]^GGCC[GA] 4487 CGGCCA Eam1105I 3659 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4283 GACTGGTTGTC Eco81I 1464 CCTGAGG CC^T[ACGT]AGG 3694 CCTGAGG EcoNI 90 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2052 CCTTTCCCAGG EcoRV 2595 GATATC GAT^ATC 3021 GATATC EcoT22I 3543 ATGCAT ATGCA^T 4607 ATGCAT FauI 867 CCCGC CCCGC(4/6) 1746 CCCGC HhaI 1027 GCGC GCG^C 2893 GCGC HinP1I 1027 GCGC G^CGC 2893 GCGC NsiI 3543 ATGCAT ATGCA^T 4607 ATGCAT PshBI 1925 ATTAAT AT^TAAT 2086 ATTAAT PstI 2642 CTGCAG CTGCA^G 2848 CTGCAG SmlI 2953 CTTAAG C^T[CT][GA]AG 3109 CTTGAG StuI 2065 AGGCCT AGG^CCT 2093 AGGCCT XbaI 1790 TCTAGA T^CTAGA 3258 TCTAGA XcmI 1189 CCAGAGAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1281 CCAGAACTGACCTGG XmnI 930 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2610 GAAGTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SpeI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII