##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI AvrII
BbeI BbrPI BbvCI BclI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I FbaI
FseI HaeII KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SnaBI StuI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 5847 GGTACC G^GTACC
AccII 4586 CGCG CG^CG
AcyI 690 GACGCC G[GA]^CG[CT]C
AhdI 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1653 GTGCAC G^TGCAC
ApaLI 1653 GTGCAC G^TGCAC
Asp700I 4188 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5847 GGTACC G^GTACC
AspEI 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1906 TGCGCA TGC^GCA
BalI 5821 TGGCCA TGG^CCA
BamHI 1847 GGATCC G^GATCC
BglII 5747 AGATCT A^GATCT
BlpI 59 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 59 GCTAAGC GC^T[ACGT]AGC
BsaHI 690 GACGCC G[GA]^CG[CT]C
BspMI 1042 ACCTGC ACCTGC(4/8)
BsrBI 3788 GAGCGG CCGCTC(-3/-3)
BsrDI 5222 CATTGC GCAATG(2/0)
BsrFI 3672 ACCGGC [GA]^CCGG[CT]
BstBI 4195 TTCGAA TT^CGAA
BstUI 4586 CGCG CG^CG
CelII 59 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3672 ACCGGC [GA]^CCGG[CT]
Eam1105I 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2111 GAATTC G^AATTC
EcoRV 2436 GATATC GAT^ATC
FauI 3790 GCGGG CCCGC(4/6)
FspI 1906 TGCGCA TGC^GCA
HgaI 690 GACGC GACGC(5/10)
Hin1I 690 GACGCC G[GA]^CG[CT]C
HpaI 1253 GTTAAC GTT^AAC
KpnI 5847 GGTACC GGTAC^C
MluNI 5821 TGGCCA TGG^CCA
MscI 5821 TGGCCA TGG^CCA
MvnI 4586 CGCG CG^CG
NspV 4195 TTCGAA TT^CGAA
PaeR7I 4911 CTCGAG C^TCGAG
PflMI 116 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 2549 GAGCTC GAGCT^C
SapI 814 GCTCTTC GCTCTTC(1/4)
SbfI 1043 CCTGCAGG CCTGCA^GG
SfuI 4195 TTCGAA TT^CGAA
SmaI 3736 CCCGGG CCC^GGG
SpeI 3404 ACTAGT A^CTAGT
Sse8387I 1043 CCTGCAGG CCTGCA^GG
SwaI 2870 ATTTAAAT ATTT^AAAT
Van91I 116 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 650 TCTAGA T^CTAGA
XhoI 4911 CTCGAG C^TCGAG
XmaCI 3736 CCCGGG C^CCGGG
XmaI 3736 CCCGGG C^CCGGG
XmnI 4188 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 741 CTTAAG C^TTAAG
2013 CTTAAG
AocI 2531 CCTGAGG CC^T[ACGT]AGG
3940 CCTCAGG
AspI 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACACTGTC
BcgI 679 GCATGGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4569 GCACCGTCGTCG
BciVI 3162 GGATAC GTATCC(6/5)
4665 GTATCC
BcnI 3736 CCCGG CC^[GC]GG
3737 CCGGG
BfrI 741 CTTAAG C^TTAAG
2013 CTTAAG
Bsp1407I 3846 TGTACA T^GTACA
4918 TGTACA
BspHI 3709 TCATGA T^CATGA
5780 TCATGA
BsrGI 3846 TGTACA T^GTACA
4918 TGTACA
BstXI 3367 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5372 CCAGCAGAATGG
Bsu36I 2531 CCTGAGG CC^T[ACGT]AGG
3940 CCTCAGG
CfoI 1907 GCGC GCG^C
4585 GCGC
DraIII 1899 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
5810 CACTGTGTG
DrdI 1300 GACAGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4956 GACGAAGGTGTC
EaeI 4486 TGGCCG [CT]^GGCC[GA]
5821 TGGCCA
Eco81I 2531 CCTGAGG CC^T[ACGT]AGG
3940 CCTCAGG
EcoNI 1055 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4052 CCTGACAGAGG
HhaI 1907 GCGC GCG^C
4585 GCGC
HinP1I 1907 GCGC G^CGC
4585 GCGC
MaeII 1917 ACGT A^CGT
2908 ACGT
NciI 3736 CCCGG CC^[GC]GG
3737 CCGGG
NdeI 2176 CATATG CA^TATG
4253 CATATG
PflFI 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACACTGTC
PshAI 1288 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3508 GACCCCAGTC
RcaI 3709 TCATGA T^CATGA
5780 TCATGA
SphI 2986 GCATGC GCATG^C
5470 GCATGC
SspBI 3846 TGTACA T^GTACA
4918 TGTACA
Tth111I 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII