################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI AvrII BbeI BbrPI BbvCI BclI BglI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I FbaI FseI HaeII KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SgrAI SnaBI StuI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 5847 GGTACC G^GTACC AccII 4586 CGCG CG^CG AcyI 690 GACGCC G[GA]^CG[CT]C AhdI 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1653 GTGCAC G^TGCAC ApaLI 1653 GTGCAC G^TGCAC Asp700I 4188 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5847 GGTACC G^GTACC AspEI 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1906 TGCGCA TGC^GCA BalI 5821 TGGCCA TGG^CCA BamHI 1847 GGATCC G^GATCC BglII 5747 AGATCT A^GATCT BlpI 59 GCTAAGC GC^T[ACGT]AGC Bpu1102I 59 GCTAAGC GC^T[ACGT]AGC BsaHI 690 GACGCC G[GA]^CG[CT]C BspMI 1042 ACCTGC ACCTGC(4/8) BsrBI 3788 GAGCGG CCGCTC(-3/-3) BsrDI 5222 CATTGC GCAATG(2/0) BsrFI 3672 ACCGGC [GA]^CCGG[CT] BstBI 4195 TTCGAA TT^CGAA BstUI 4586 CGCG CG^CG CelII 59 GCTAAGC GC^T[ACGT]AGC Cfr10I 3672 ACCGGC [GA]^CCGG[CT] Eam1105I 2716 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2111 GAATTC G^AATTC EcoRV 2436 GATATC GAT^ATC FauI 3790 GCGGG CCCGC(4/6) FspI 1906 TGCGCA TGC^GCA HgaI 690 GACGC GACGC(5/10) Hin1I 690 GACGCC G[GA]^CG[CT]C HpaI 1253 GTTAAC GTT^AAC KpnI 5847 GGTACC GGTAC^C MluNI 5821 TGGCCA TGG^CCA MscI 5821 TGGCCA TGG^CCA MvnI 4586 CGCG CG^CG NspV 4195 TTCGAA TT^CGAA PaeR7I 4911 CTCGAG C^TCGAG PflMI 116 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 2549 GAGCTC GAGCT^C SapI 814 GCTCTTC GCTCTTC(1/4) SbfI 1043 CCTGCAGG CCTGCA^GG SfuI 4195 TTCGAA TT^CGAA SmaI 3736 CCCGGG CCC^GGG SpeI 3404 ACTAGT A^CTAGT Sse8387I 1043 CCTGCAGG CCTGCA^GG SwaI 2870 ATTTAAAT ATTT^AAAT Van91I 116 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 650 TCTAGA T^CTAGA XhoI 4911 CTCGAG C^TCGAG XmaCI 3736 CCCGGG C^CCGGG XmaI 3736 CCCGGG C^CCGGG XmnI 4188 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflII 741 CTTAAG C^TTAAG 2013 CTTAAG AocI 2531 CCTGAGG CC^T[ACGT]AGG 3940 CCTCAGG AspI 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3646 GACACTGTC BcgI 679 GCATGGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4569 GCACCGTCGTCG BciVI 3162 GGATAC GTATCC(6/5) 4665 GTATCC BcnI 3736 CCCGG CC^[GC]GG 3737 CCGGG BfrI 741 CTTAAG C^TTAAG 2013 CTTAAG Bsp1407I 3846 TGTACA T^GTACA 4918 TGTACA BspHI 3709 TCATGA T^CATGA 5780 TCATGA BsrGI 3846 TGTACA T^GTACA 4918 TGTACA BstXI 3367 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5372 CCAGCAGAATGG Bsu36I 2531 CCTGAGG CC^T[ACGT]AGG 3940 CCTCAGG CfoI 1907 GCGC GCG^C 4585 GCGC DraIII 1899 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 5810 CACTGTGTG DrdI 1300 GACAGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4956 GACGAAGGTGTC EaeI 4486 TGGCCG [CT]^GGCC[GA] 5821 TGGCCA Eco81I 2531 CCTGAGG CC^T[ACGT]AGG 3940 CCTCAGG EcoNI 1055 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4052 CCTGACAGAGG HhaI 1907 GCGC GCG^C 4585 GCGC HinP1I 1907 GCGC G^CGC 4585 GCGC MaeII 1917 ACGT A^CGT 2908 ACGT NciI 3736 CCCGG CC^[GC]GG 3737 CCGGG NdeI 2176 CATATG CA^TATG 4253 CATATG PflFI 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3646 GACACTGTC PshAI 1288 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3508 GACCCCAGTC RcaI 3709 TCATGA T^CATGA 5780 TCATGA SphI 2986 GCATGC GCATG^C 5470 GCATGC SspBI 3846 TGTACA T^GTACA 4918 TGTACA Tth111I 1136 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3646 GACACTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII