################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AclI AflII AgeI AvrII BamHI BclI BfrI BlnI BsiWI BsmI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI DraI DrdI EcoRV EcoT22I FbaI FseI HindIII MfeI MluI MunI NdeI NheI NotI NsiI NspV PaeR7I PflMI PinAI PmeI PshAI Psp1406I RcaI SacI SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SspBI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 4029 GGTACC G^GTACC AccIII 17 TCCGGA T^CCGGA AcsI 3120 GAATTC [GA]^AATT[CT] AocI 2946 CCTGAGG CC^T[ACGT]AGG Aor51HI 3247 AGCGCT AGC^GCT ApoI 3120 GAATTC [GA]^AATT[CT] AscI 94 GGCGCGCC GG^CGCGCC AseI 4369 ATTAAT AT^TAAT AsnI 4369 ATTAAT AT^TAAT Asp700I 4148 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4029 GGTACC G^GTACC AspI 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1729 TGCGCA TGC^GCA BbeI 2951 GGCGCC GGCGC^C BciVI 1157 GGATAC GTATCC(6/5) BglII 1681 AGATCT A^GATCT BsaBI 3896 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 17 TCCGGA T^CCGGA BsmBI 4247 GAGACG CGTCTC(1/5) BspEI 17 TCCGGA T^CCGGA BsrBI 263 GAGCGG CCGCTC(-3/-3) BssHII 95 GCGCGC G^CGCGC BstEII 3284 GGTGACC G^GT[ACGT]ACC BstPI 3284 GGTGACC G^GT[ACGT]ACC BstXI 123 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2946 CCTGAGG CC^T[ACGT]AGG CpoI 178 CGGTCCG CG^G[AT]CCG Eco47III 3247 AGCGCT AGC^GCT Eco81I 2946 CCTGAGG CC^T[ACGT]AGG EcoNI 728 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3284 GGTGACC G^GT[ACGT]ACC EcoRI 3120 GAATTC G^AATTC FspI 1729 TGCGCA TGC^GCA HincII 4615 GTTAAC GT[CT]^[GA]AC HindII 4615 GTTAAC GT[CT]^[GA]AC HpaI 4615 GTTAAC GTT^AAC KasI 2951 GGCGCC G^GCGCC KpnI 4029 GGTACC GGTAC^C MamI 3896 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 17 TCCGGA T^CCGGA NarI 2951 GGCGCC GG^CGCC NruI 4212 TCGCGA TCG^CGA PacI 4702 TTAATTAA TTAAT^TAA PflFI 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4369 ATTAAT AT^TAAT PvuI 354 CGATCG CGAT^CG RsrII 178 CGGTCCG CG^G[AT]CCG SexAI 3583 ACCTGGT A^CC[AT]GGT SfoI 2951 GGCGCC GGC^GCC SphI 2929 GCATGC GCATG^C Tth111I 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 4148 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 2175 GACGTC GACGT^C 3824 GACGTC AccI 1572 GTCTAC GT^[AC][GT]AC 2034 GTCTAC AhdI 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 573 GACTTCGAGTC AspEI 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 573 GACTTCGAGTC BbrPI 1191 CACGTG CAC^GTG 1673 CACGTG BbvCI 1659 CCTCAGC CCTCAGC(-5/-2) 3744 GCTGAGG BcgI 199 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1808 CGAGATGTTTGC BlpI 2151 GCTGAGC GC^T[ACGT]AGC 3496 GCTCAGC Bpu1102I 2151 GCTGAGC GC^T[ACGT]AGC 3496 GCTCAGC BsaWI 17 TCCGGA [AT]^CCGG[AT] 4741 ACCGGA BspLU11I 325 ACATGT A^CATGT 3226 ACATGT BsrDI 73 CATTGC GCAATG(2/0) 1968 CATTGC BssSI 939 CACGAG CACGAG(-5/-1) 2964 CACGAG CelII 2151 GCTGAGC GC^T[ACGT]AGC 3496 GCTCAGC EagI 4 CGGCCG C^GGCCG 192 CGGCCG Eam1105I 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 573 GACTTCGAGTC EclXI 4 CGGCCG C^GGCCG 192 CGGCCG Eco52I 4 CGGCCG C^GGCCG 192 CGGCCG KspI 292 CCGCGG CCGC^GG 2857 CCGCGG NaeI 3627 GCCGGC GCC^GGC 3702 GCCGGC NgoMIV 3627 GCCGGC G^CCGGC 3702 GCCGGC PmaCI 1191 CACGTG CAC^GTG 1673 CACGTG PmlI 1191 CACGTG CAC^GTG 1673 CACGTG SacII 292 CCGCGG CCGC^GG 2857 CCGCGG SmaI 103 CCCGGG CCC^GGG 167 CCCGGG SspI 4366 AATATT AAT^ATT 4683 AATATT XmaCI 103 CCCGGG C^CCGGG 167 CCCGGG XmaI 103 CCCGGG C^CCGGG 167 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII