##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AflII AgeI AvrII BamHI
BclI BfrI BlnI BsiWI BsmI
Bsp1407I BspDI BspHI BsrGI Bst1107I
BstBI BstZ17I ClaI DraI DrdI
EcoRV EcoT22I FbaI FseI HindIII
MfeI MluI MunI NdeI NheI
NotI NsiI NspV PaeR7I PflMI
PinAI PmeI PshAI Psp1406I RcaI
SacI SalI SapI ScaI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4029 GGTACC G^GTACC
AccIII 17 TCCGGA T^CCGGA
AcsI 3120 GAATTC [GA]^AATT[CT]
AocI 2946 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3247 AGCGCT AGC^GCT
ApoI 3120 GAATTC [GA]^AATT[CT]
AscI 94 GGCGCGCC GG^CGCGCC
AseI 4369 ATTAAT AT^TAAT
AsnI 4369 ATTAAT AT^TAAT
Asp700I 4148 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4029 GGTACC G^GTACC
AspI 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1729 TGCGCA TGC^GCA
BbeI 2951 GGCGCC GGCGC^C
BciVI 1157 GGATAC GTATCC(6/5)
BglII 1681 AGATCT A^GATCT
BsaBI 3896 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 17 TCCGGA T^CCGGA
BsmBI 4247 GAGACG CGTCTC(1/5)
BspEI 17 TCCGGA T^CCGGA
BsrBI 263 GAGCGG CCGCTC(-3/-3)
BssHII 95 GCGCGC G^CGCGC
BstEII 3284 GGTGACC G^GT[ACGT]ACC
BstPI 3284 GGTGACC G^GT[ACGT]ACC
BstXI 123 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2946 CCTGAGG CC^T[ACGT]AGG
CpoI 178 CGGTCCG CG^G[AT]CCG
Eco47III 3247 AGCGCT AGC^GCT
Eco81I 2946 CCTGAGG CC^T[ACGT]AGG
EcoNI 728 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3284 GGTGACC G^GT[ACGT]ACC
EcoRI 3120 GAATTC G^AATTC
FspI 1729 TGCGCA TGC^GCA
HincII 4615 GTTAAC GT[CT]^[GA]AC
HindII 4615 GTTAAC GT[CT]^[GA]AC
HpaI 4615 GTTAAC GTT^AAC
KasI 2951 GGCGCC G^GCGCC
KpnI 4029 GGTACC GGTAC^C
MamI 3896 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 17 TCCGGA T^CCGGA
NarI 2951 GGCGCC GG^CGCC
NruI 4212 TCGCGA TCG^CGA
PacI 4702 TTAATTAA TTAAT^TAA
PflFI 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4369 ATTAAT AT^TAAT
PvuI 354 CGATCG CGAT^CG
RsrII 178 CGGTCCG CG^G[AT]CCG
SexAI 3583 ACCTGGT A^CC[AT]GGT
SfoI 2951 GGCGCC GGC^GCC
SphI 2929 GCATGC GCATG^C
Tth111I 3582 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4148 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2175 GACGTC GACGT^C
3824 GACGTC
AccI 1572 GTCTAC GT^[AC][GT]AC
2034 GTCTAC
AhdI 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
573 GACTTCGAGTC
AspEI 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
573 GACTTCGAGTC
BbrPI 1191 CACGTG CAC^GTG
1673 CACGTG
BbvCI 1659 CCTCAGC CCTCAGC(-5/-2)
3744 GCTGAGG
BcgI 199 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1808 CGAGATGTTTGC
BlpI 2151 GCTGAGC GC^T[ACGT]AGC
3496 GCTCAGC
Bpu1102I 2151 GCTGAGC GC^T[ACGT]AGC
3496 GCTCAGC
BsaWI 17 TCCGGA [AT]^CCGG[AT]
4741 ACCGGA
BspLU11I 325 ACATGT A^CATGT
3226 ACATGT
BsrDI 73 CATTGC GCAATG(2/0)
1968 CATTGC
BssSI 939 CACGAG CACGAG(-5/-1)
2964 CACGAG
CelII 2151 GCTGAGC GC^T[ACGT]AGC
3496 GCTCAGC
EagI 4 CGGCCG C^GGCCG
192 CGGCCG
Eam1105I 172 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
573 GACTTCGAGTC
EclXI 4 CGGCCG C^GGCCG
192 CGGCCG
Eco52I 4 CGGCCG C^GGCCG
192 CGGCCG
KspI 292 CCGCGG CCGC^GG
2857 CCGCGG
NaeI 3627 GCCGGC GCC^GGC
3702 GCCGGC
NgoMIV 3627 GCCGGC G^CCGGC
3702 GCCGGC
PmaCI 1191 CACGTG CAC^GTG
1673 CACGTG
PmlI 1191 CACGTG CAC^GTG
1673 CACGTG
SacII 292 CCGCGG CCGC^GG
2857 CCGCGG
SmaI 103 CCCGGG CCC^GGG
167 CCCGGG
SspI 4366 AATATT AAT^ATT
4683 AATATT
XmaCI 103 CCCGGG C^CCGGG
167 CCCGGG
XmaI 103 CCCGGG C^CCGGG
167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII