##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BcgI BglI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrFI BssHII
BstBI BstUI Bsu36I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I FseI FspI HgaI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflMI PinAI PmeI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AspI 711 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1056 GCTGAGG CCTCAGC(-5/-2)
BclI 2159 TGATCA T^GATCA
BcnI 364 CCCGG CC^[GC]GG
BlpI 3128 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3128 GCTTAGC GC^T[ACGT]AGC
BspHI 4882 TCATGA T^CATGA
BsrBI 1610 CCGCTC CCGCTC(-3/-3)
Bst1107I 5552 GTATAC GTA^TAC
BstEII 4382 GGTTACC G^GT[ACGT]ACC
BstPI 4382 GGTTACC G^GT[ACGT]ACC
BstXI 4979 CCACTCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5552 GTATAC GTA^TAC
CelII 3128 GCTTAGC GC^T[ACGT]AGC
DrdI 2435 GACTCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4382 GGTTACC G^GT[ACGT]ACC
EcoRI 4923 GAATTC G^AATTC
EcoRV 26 GATATC GAT^ATC
FauI 1924 CCCGC CCCGC(4/6)
FbaI 2159 TGATCA T^GATCA
HapII 365 CCGG C^CGG
HpaII 365 CCGG C^CGG
MspI 365 CCGG C^CGG
NciI 364 CCCGG CC^[GC]GG
PacI 4045 TTAATTAA TTAAT^TAA
PflFI 711 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 865 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1521 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4882 TCATGA T^CATGA
SexAI 129 ACCAGGT A^CC[AT]GGT
SpeI 3928 ACTAGT A^CTAGT
TaqI 2039 TCGA T^CGA
Tth111I 711 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2039 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 206 AACGTT AA^CGTT
2820 AACGTT
AcyI 1821 GGCGCC G[GA]^CG[CT]C
3921 GGCGCC
AflII 2021 CTTAAG C^TTAAG
2472 CTTAAG
Asp700I 486 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAAGTCTTTC
BalI 1033 TGGCCA TGG^CCA
1859 TGGCCA
BbeI 1821 GGCGCC GGCGC^C
3921 GGCGCC
BbrPI 4601 CACGTG CAC^GTG
5013 CACGTG
BciVI 640 GTATCC GTATCC(6/5)
4289 GTATCC
BfrI 2021 CTTAAG C^TTAAG
2472 CTTAAG
BglII 2321 AGATCT A^GATCT
5311 AGATCT
BsaHI 1821 GGCGCC G[GA]^CG[CT]C
3921 GGCGCC
BsaI 952 GAGACC GGTCTC(1/5)
2888 GGTCTC
Bsp1407I 3232 TGTACA T^GTACA
4500 TGTACA
BspMI 500 ACCTGC ACCTGC(4/8)
4614 ACCTGC
BsrGI 3232 TGTACA T^GTACA
4500 TGTACA
BssSI 2554 CTCGTG CACGAG(-5/-1)
4984 CTCGTG
DraIII 1356 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1572 CACAGGGTG
EcoNI 2625 CCTGTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2784 CCTCTGCCAGG
Hin1I 1821 GGCGCC G[GA]^CG[CT]C
3921 GGCGCC
HincII 1528 GTCAAC GT[CT]^[GA]AC
4320 GTTGAC
HindII 1528 GTCAAC GT[CT]^[GA]AC
4320 GTTGAC
KasI 1821 GGCGCC G^GCGCC
3921 GGCGCC
MluNI 1033 TGGCCA TGG^CCA
1859 TGGCCA
MscI 1033 TGGCCA TGG^CCA
1859 TGGCCA
NarI 1821 GGCGCC GG^CGCC
3921 GGCGCC
PmaCI 4601 CACGTG CAC^GTG
5013 CACGTG
PmlI 4601 CACGTG CAC^GTG
5013 CACGTG
Psp1406I 206 AACGTT AA^CGTT
2820 AACGTT
SacI 261 GAGCTC GAGCT^C
552 GAGCTC
SfoI 1821 GGCGCC GGC^GCC
3921 GGCGCC
SspBI 3232 TGTACA T^GTACA
4500 TGTACA
StuI 2281 AGGCCT AGG^CCT
4347 AGGCCT
SwaI 3709 ATTTAAAT ATTT^AAAT
3751 ATTTAAAT
XmnI 486 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1468 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII