##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI Asp718I AvaI
AviII AvrII BbeI BlnI BsaBI
BseAI BsmI BsoBI BspDI BspEI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoNI FseI FspI HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 133 GACGCC G[GA]^CG[CT]C
AflII 4825 CTTAAG C^TTAAG
AocI 1160 CCTCAGG CC^T[ACGT]AGG
ApaI 864 GGGCCC GGGCC^C
AspI 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4680 TGGCCA TGG^CCA
BbrPI 6205 CACGTG CAC^GTG
BcgI 3895 CGACCGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4825 CTTAAG C^TTAAG
BsaAI 6205 CACGTG [CT]AC^GT[GA]
BsaHI 133 GACGCC G[GA]^CG[CT]C
BsiEI 3895 CGACCG CG[GA][CT]^CG
BsiWI 2041 CGTACG C^GTACG
BsmBI 1220 CGTCTC CGTCTC(1/5)
BspHI 400 TCATGA T^CATGA
BssSI 672 CACGAG CACGAG(-5/-1)
BstBI 1568 TTCGAA TT^CGAA
BstEII 4858 GGTGACC G^GT[ACGT]ACC
BstPI 4858 GGTGACC G^GT[ACGT]ACC
BstXI 1141 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1160 CCTCAGG CC^T[ACGT]AGG
DrdI 4030 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1160 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4858 GGTGACC G^GT[ACGT]ACC
EcoRV 3287 GATATC GAT^ATC
EcoT22I 5075 ATGCAT ATGCA^T
Hin1I 133 GACGCC G[GA]^CG[CT]C
KspI 181 CCGCGG CCGC^GG
MluNI 4680 TGGCCA TGG^CCA
MscI 4680 TGGCCA TGG^CCA
NruI 352 TCGCGA TCG^CGA
NsiI 5075 ATGCAT ATGCA^T
NspV 1568 TTCGAA TT^CGAA
PflFI 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3387 CCATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 6205 CACGTG CAC^GTG
PmlI 6205 CACGTG CAC^GTG
PshAI 1470 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 400 TCATGA T^CATGA
SacII 181 CCGCGG CCGC^GG
ScaI 1768 AGTACT AGT^ACT
SfuI 1568 TTCGAA TT^CGAA
SpeI 635 ACTAGT A^CTAGT
SwaI 5248 ATTTAAAT ATTT^AAAT
Tth111I 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3387 CCATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 6006 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1005 GTAGAC GT^[AC][GT]AC
2637 GTAGAC
AhdI 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5436 GACTTCCCGTC
Aor51HI 84 AGCGCT AGC^GCT
731 AGCGCT
AspEI 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5436 GACTTCCCGTC
BbvCI 2067 GCTGAGG CCTCAGC(-5/-2)
3015 GCTGAGG
BciVI 1444 GTATCC GTATCC(6/5)
5330 GTATCC
BsaI 415 GAGACC GGTCTC(1/5)
4075 GGTCTC
Bsp1407I 2584 TGTACA T^GTACA
6377 TGTACA
BsrBI 1197 GAGCGG CCGCTC(-3/-3)
3645 GAGCGG
BsrGI 2584 TGTACA T^GTACA
6377 TGTACA
DraIII 4441 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
4670 CACACAGTG
Eam1105I 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5436 GACTTCCCGTC
Eco47III 84 AGCGCT AGC^GCT
731 AGCGCT
PacI 3767 TTAATTAA TTAAT^TAA
6043 TTAATTAA
PpuMI 2323 AGGACCC [GA]G^G[AT]CC[CT]
3882 GGGTCCC
PstI 747 CTGCAG CTGCA^G
1428 CTGCAG
SacI 1608 GAGCTC GAGCT^C
4207 GAGCTC
SmlI 2280 CTCAAG C^T[CT][GA]AG
4825 CTTAAG
SspBI 2584 TGTACA T^GTACA
6377 TGTACA
SspI 3299 AATATT AAT^ATT
6434 AATATT
StuI 1273 AGGCCT AGG^CCT
5577 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BamHI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI