################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I ApaLI AscI Asp718I AvaI AviII AvrII BbeI BlnI BsaBI BseAI BsmI BsoBI BspDI BspEI BsrFI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I EcoNI FseI FspI HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 133 GACGCC G[GA]^CG[CT]C AflII 4825 CTTAAG C^TTAAG AocI 1160 CCTCAGG CC^T[ACGT]AGG ApaI 864 GGGCCC GGGCC^C AspI 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4680 TGGCCA TGG^CCA BbrPI 6205 CACGTG CAC^GTG BcgI 3895 CGACCGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4825 CTTAAG C^TTAAG BsaAI 6205 CACGTG [CT]AC^GT[GA] BsaHI 133 GACGCC G[GA]^CG[CT]C BsiEI 3895 CGACCG CG[GA][CT]^CG BsiWI 2041 CGTACG C^GTACG BsmBI 1220 CGTCTC CGTCTC(1/5) BspHI 400 TCATGA T^CATGA BssSI 672 CACGAG CACGAG(-5/-1) BstBI 1568 TTCGAA TT^CGAA BstEII 4858 GGTGACC G^GT[ACGT]ACC BstPI 4858 GGTGACC G^GT[ACGT]ACC BstXI 1141 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1160 CCTCAGG CC^T[ACGT]AGG DrdI 4030 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1160 CCTCAGG CC^T[ACGT]AGG EcoO65I 4858 GGTGACC G^GT[ACGT]ACC EcoRV 3287 GATATC GAT^ATC EcoT22I 5075 ATGCAT ATGCA^T Hin1I 133 GACGCC G[GA]^CG[CT]C KspI 181 CCGCGG CCGC^GG MluNI 4680 TGGCCA TGG^CCA MscI 4680 TGGCCA TGG^CCA NruI 352 TCGCGA TCG^CGA NsiI 5075 ATGCAT ATGCA^T NspV 1568 TTCGAA TT^CGAA PflFI 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3387 CCATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 6205 CACGTG CAC^GTG PmlI 6205 CACGTG CAC^GTG PshAI 1470 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 400 TCATGA T^CATGA SacII 181 CCGCGG CCGC^GG ScaI 1768 AGTACT AGT^ACT SfuI 1568 TTCGAA TT^CGAA SpeI 635 ACTAGT A^CTAGT SwaI 5248 ATTTAAAT ATTT^AAAT Tth111I 738 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3387 CCATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 6006 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 1005 GTAGAC GT^[AC][GT]AC 2637 GTAGAC AhdI 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5436 GACTTCCCGTC Aor51HI 84 AGCGCT AGC^GCT 731 AGCGCT AspEI 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5436 GACTTCCCGTC BbvCI 2067 GCTGAGG CCTCAGC(-5/-2) 3015 GCTGAGG BciVI 1444 GTATCC GTATCC(6/5) 5330 GTATCC BsaI 415 GAGACC GGTCTC(1/5) 4075 GGTCTC Bsp1407I 2584 TGTACA T^GTACA 6377 TGTACA BsrBI 1197 GAGCGG CCGCTC(-3/-3) 3645 GAGCGG BsrGI 2584 TGTACA T^GTACA 6377 TGTACA DraIII 4441 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG 4670 CACACAGTG Eam1105I 3212 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5436 GACTTCCCGTC Eco47III 84 AGCGCT AGC^GCT 731 AGCGCT PacI 3767 TTAATTAA TTAAT^TAA 6043 TTAATTAA PpuMI 2323 AGGACCC [GA]G^G[AT]CC[CT] 3882 GGGTCCC PstI 747 CTGCAG CTGCA^G 1428 CTGCAG SacI 1608 GAGCTC GAGCT^C 4207 GAGCTC SmlI 2280 CTCAAG C^T[CT][GA]AG 4825 CTTAAG SspBI 2584 TGTACA T^GTACA 6377 TGTACA SspI 3299 AATATT AAT^ATT 6434 AATATT StuI 1273 AGGCCT AGG^CCT 5577 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI